Name: mesenteric artery contraction and relaxation studies using automated wire myography
Uploaded: 2011-09-22T13:00:00
Description: Watch this Scientific Journal Video about Mesenteric Artery Contraction and Relaxation Studies Using Automated Wire Myography at JoVE.com

描述该项目奖号码R01 HL064761，R25 HL059868 1SC1 HL099139和P20 MD000175形式国立卫生研究院的支持。内容完全是作者的责任，并不一定代表国立卫生研究院的官方意见。

1。分离大鼠肠系膜小动脉<ol><li>麻醉与异氟醚在一个封闭的腔的动物，并用酒精擦拭腹部。 </li><li>执行行剖腹探查术中暴露肠系膜床。 </li><li>用剪刀，除去约85厘米的肠道喂养与肠系膜上动脉血管。切割近端肠道部分接近幽门和末端回肠coecal交界附近。部分小肠被隔离的大鼠，深感麻醉与异氟醚和开胸心脏穿刺安乐死。 </li><li>涂层的培养皿含PSS的菜摆在切除部分和肠系膜上动脉，在室温下进?...

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高血压是心血管，脑和肾脏的发病率/死亡率的首要原因。高血压的发生是在人口高盐敏感性高血压是特别高，在人口老龄化，更多的黑人比白人流行。这被认为是由于对黑人的倾向保留在自己的肾脏 9钠。盐敏感性是肾脏疾病的一个主要因素，并与血管内皮功能障碍有关，但机制尚不完全清楚。在我们实验室最近的研究显示，高盐饮食可减少组织间液的Ca 2 +浓度（[Ca2 2 <su...

<table border="1"><tbody><tr><th>设备名称/试剂</th><th>公司</th><th>目录编号</th><th>评论（可选） </th></tr><tr><td>自动双丝肌动描记系统- 510A DMT美国公司</td><td>佐治亚州亚特兰大。 </td><td> 100151 </td><td></td></tr><tr><td> PowerLab/4SP数据采集系统</td><td> ADInstruments，科罗拉多州的斯普林斯，CO </td><td> ML750 </td><td> 4-16输入通道的新车型。 </td></tr><tr><td>戴尔Dimension XPS第四代计算机</td><td>戴尔</td><td></td><td></td></tr><tr><td> STEMI SV二（APO）与眼的解剖显微镜</td><td>国际卡尔蔡司。 </td><td></td><td></td></tr><tr><td>只Wistar，Dahl盐敏感，Dahl盐抵抗大鼠</td><td>哈伦只Dawley，印第安纳波利斯，IN。 </td><td></td><td></td></tr><tr><td>鼠类议员</td><td>哈兰德Tekland，麦迪逊，威斯康星州。 </td><td></td><td></td></tr><tr><td>去氧</td><td> Sigma - Aldrich公司，圣路易斯，密苏里州。 </td><td></td><td></td></tr><tr><td>其他化学品</td><td></td><td></td><td>所有使用的化学品是最纯净的成绩可以买到。 </td></tr></tbody></table>

mesenteric artery contraction and relaxation studies using automated wire myography

如肠系膜动脉的近端阻力血管，作出重大贡献的外周阻力。这些小血管的功能，主要是在指挥根据到身体的总体要求各个器官的血流量，直径100-400微米之间。大鼠肠系膜动脉的直径大于100微米的。贝文和奥谢尔2所提出的方法的基础上，首先由 1 Mulvay和哈尔彭所描述的，是myography技术。该技术提供等距条件下，大幅缩短的肌肉准备阻止的小型船只的信息。由于力的生产和船只不同受体激动剂的敏感性依赖于伸展的程度，按照积极紧张长度关系，这是必不可少的等距条件下进行收缩的研究，以防止符合安装电线。不锈钢丝是首选，因为后者的氧化，从而影响记录反应3钨丝，该技术允许安装船只激动剂诱导的收缩比较，以获取证据表明血管平滑肌细胞受体的正常功能。 在几项研究中，我们已经表明phenylyephrine承包的，孤立的肠系膜动脉累积浓度的钙（钙2 + E）除了放宽。结果导致我们得出结论，它表达的G蛋白偶联的Ca 2 +敏感受体（CAR），血管周围的感官神经，调解这个血管舒张反应。使用自动线myography方法，我们在这里展示，从只Wistar Dahl盐敏感（DS）和Dahl盐抵抗（DR）的肠系膜动脉的大鼠有不同的反应的Ca 2 + E 。从大鼠组织表现出更高的Ca 2 +敏感性相比，DR和DS。减少从DS大鼠肠系膜动脉车表达与减少的Ca 2 + E -诱导孤立的，承包前动脉松弛。数据表明，汽车是需要放宽增加肾上腺素基调下肠系膜动脉，在高血压发生，并表示在汽车的信号转导通路在达尔动物的固有缺陷，这是更为严重的DS。 肠系膜阻力动脉和类似的小血管和比较不同的受体激动剂和/或拮抗剂在确定血管反应性的体外的方法是有用的，可以很容易地和一贯的评估并排侧6,7,8。

Watch this Scientific Journal Video about Mesenteric Artery Contraction and Relaxation Studies Using Automated Wire Myography at JoVE.com

Mesenteric Artery Contraction and Relaxation Studies Using Automated Wire Myography

一个孤立的肠系膜动脉的力测量的自动化myography方法描述。它采用一个Mulvany哈尔彭自动双丝肌动描记510A福林和钙，以确定响应。该方法允许等距反应一致，以激动剂决心在小血管的直径为60 - 300微米，独立。

肠系膜动脉收缩和舒张的研究，使用自动线Myography

Proximal resistance vessels, such as the mesenteric arteries, contribute substantially to the peripheral resistance. These small vessels of between ...

Research

JoVE Journal

Medicine

Guide Wire Assisted Catheterization and Colored Dye Injection for Vascular Mapping of Monochorionic Twin Placentas

导丝辅助导管和彩色染料注射的双人单绒毛膜胎盘血管映射

Monochorionic (MC) twin pregnancies are associated with significantly higher morbidity and mortality rates than dichorionic twins. Approximately 50% of MC ...

科研

医学

Assessing Myogenic Response and Vasoactivity In Resistance Mesenteric Arteries Using Pressure Myography

评估肌反应及血管活性阻力肠系膜动脉使用压力肌动描记

Small resistance arteries constrict and dilate respectively in response to increased or decreased intraluminal pressure; this phenomenon known as myogenic ...

Isolating and Using Sections of Bovine Mesenteric Artery and Vein as a Bioassay to Test for Vasoactivity in the Small Intestine

隔离和使用的牛肠系膜动脉和静脉部分，作为生物测定在小肠测试的Vasoactivity

Mammalian gastrointestinal systems are constantly exposed to compounds (desirable and undesirable) that can have an effect on blood flow to and from that ...

Murine Model of Femoral Artery Wire Injury with Implantation of a Perivascular Drug Delivery Patch

股动脉线损伤的小鼠模型的血管周围给药补丁植入

Percutaneous interventions including balloon angioplasty and stenting have been used to restore blood flow in vessels with occlusive vascular disease. ...

A Murine Model of Arterial Restenosis: Technical Aspects of Femoral Wire Injury

动脉再狭窄小鼠模型：股导线损伤的技术方面

Cardiovascular disease caused by atherosclerosis is the leading cause of death in the developed world. Narrowing of the vessel lumen, due to ...

Induction of Accelerated Atherosclerosis in Mice: The "Wire-Injury" Model

小鼠加速动脉硬化的诱导："电线损伤"模型

Atherosclerosis is a proliferative fibro-inflammatory disease developing in the arterial wall, inducing a deficient blood flow or a lack of blood flow. ...

Laparoscopic Technique for Serial Collection of Liver and Mesenteric Lymph Nodes in Macaques

腹腔镜技术在猕猴肝和肠系膜淋巴结的串行采集

The mesenteric lymph nodes (MLN) and the liver are exposed to microbes and microbial products from the gastrointestinal (GI) tract, making them ...

Isometric Contractility Measurement of the Mouse Mesenteric Artery Using Wire Myography

用线 Myography 测定小鼠肠系膜动脉的等轴收缩力

The wire myograph technique is used to assess the contractility of vascular smooth muscles in response to depolarization, GPCR agonists/inhibitors and ...

Brachial Artery Catheterization in Swine

猪的臂动脉插管

The video describes in detail the catheterization of the distal brachial artery in swine. This technique enables researchers to measure arterial blood ...

Murine Myocardial Infarction Model using Permanent Ligation of Left Anterior Descending Coronary Artery

使用左前部降冠动脉的永久结扎的月心肌梗死模型

Myocardial infarction (MI) and acute coronary diseases are among the most prominent causes of death in population with western lifestyle. The murine ...