Name: mesenteric artery contraction and relaxation studies using automated wire myography
Uploaded: 2011-09-22T13:00:00
Description: Watch this Scientific Journal Video about Mesenteric Artery Contraction and Relaxation Studies Using Automated Wire Myography at JoVE.com

Проекта, описанного была поддержана премии номера R01 HL064761, R25 HL059868, 1SC1 HL099139 и P20 MD000175 формы Национальных Институтов Здоровья. Содержание несут их авторы и не обязательно отражает официальную точку зрения Национального института здоровья.

1. Выделение крысы брыжеечной артерии небольшого <ol><li> Обезболить животное с изофлуран в закрытую камеру и протрите живот спиртом. </li><li> Выполните средней линии лапаротомии выявить брыжеечной постели. </li><li> Использование ножницы, удалить около 85 см кишечника с кормлением сосудистую с ...

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	<li>Mulvany, M. J., Halpern, W. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Mechanical+properties+of+vascular+smooth+muscle+cells+in+situ.">Mechanical properties of vascular smooth muscle cells in situ.</a> Nature. 260, 617-619 (1976).</li><li>Bevan, J. A., Osher, J. V. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=A+direct+method+for+recording+tension+changes+in+the+wall+of+small+blood+vessels+in+vitro.">A direct method for recording tension changes in the wall of small blood vessels in vitro.</a> Agents Actions. 2, 257-260 (1972).</li><li>Mulvany, M. J. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Procedures+for+investigating+of+small+vessels+using+small+vessel+myograph.">Procedures for investigating of small vessels using small vessel myograph.</a> DMT Danish Myo Technology. , (2004).</li><li>Angus, J. A., Wright, C. E. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Techniques+to+study+the+pharmacodynamics+of+isolated+large+and+small+blood+vessels.">Techniques to study the pharmacodynamics of isolated large and small blood vessels.</a> J. Pharmacol. Toxicol. Methods. 44, 395-407 (2000).</li><li>Halpern, W., Mulvany, M. J., Warshaw, D. M. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Mechanical+properties+of+smooth+muscle+cells+in+the+walls+of+arterial+resistance+vessels.">Mechanical properties of smooth muscle cells in the walls of arterial resistance vessels.</a> J. Physiol. 275, 85-101 (1978).</li><li>Lindhorst, J., Alexander, N., Blignaut, J., Rayner, B. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Differences+in+hypertension+between+blacks+and+whites:+an+overview.">Differences in hypertension between blacks and whites: an overview.</a> Cardiovasc. J. Afr. 18, 241-247 (2007).</li><li>Eley, S. L., Allen, C. M., Williams, C. L., Bukosi, R. D., Pointer, M. A. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Action+of+thiazide+on+renal+interstitial+calcium.">Action of thiazide on renal interstitial calcium.</a> Am. J. Hypertens. 21, 814-819 (2008).</li><li>Palmer, C. E., Rudd, M. A., Bujoski, R. D. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Renal+interstitial+Ca2++during+sodium+loading+of+normotensive+and+Dal-salt+hypertensive+rats.">Renal interstitial Ca2+ during sodium loading of normotensive and Dal-salt hypertensive rats.</a> Am. J. Hypertens. 16, 771-776 (2003).</li><li>Hurwitz, S. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Homeostatic+control+of+plasma+calcium+concentration.">Homeostatic control of plasma calcium concentration.</a> Crit. Rev. Biochem. Mol. Biol. 31, 41-100 (1996).</li><li>Brown, E. M., MacLeod, R. J. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Extracellular+sensing+and+extracellular+calcium+signaling.">Extracellular sensing and extracellular calcium signaling.</a> Physiol. Rev. 81, 239-297 (2001).</li><li>Breitwieser, G. E. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Extracellular+calcium+as+an+integrator+of+tissue+function.">Extracellular calcium as an integrator of tissue function.</a> Int. J. Biochem. Cell Biol. 40, 1467-1480 (2008).</li><li>Mupanomunda, M. M., Wang, Y., Bukoski, R. D. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Effect+of+chronic+sensory+denervation+on+Ca2+-induced+relaxation+of+isolated+mesenteric+resistance+arteries.">Effect of chronic sensory denervation on Ca2+-induced relaxation of isolated mesenteric resistance arteries.</a> Am. J. Physiol. 274, 1655-1661 (1998).</li><li>Mupanomunda, M. M., Ishioka, N., Bukoski, R. D. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Interstitial+Ca2++undergoes+dynamic+changes+sufficient+to+stimulate+nerve-dependent+Ca2+-induced+relaxation.">Interstitial Ca2+ undergoes dynamic changes sufficient to stimulate nerve-dependent Ca2+-induced relaxation.</a> Am. J. Physiol. 276, 1035-1042 (1999).</li><li>Mupanomunda, M. M., Tian, B., Ishioka, N., Bukoski, R. D. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Renal+interstitial+Ca2+.">Renal interstitial Ca2+.</a> Am. J. Physiol. Renal Physiol. 278, F644-F649 (2000).</li><li>Awumey, E. M., Hill, S. K., Diz, D. I., Bukoski, R. D. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Cytochrome+P-450+metabolites+of+2-arachidonoylglycerol+play+a+role+in+Ca2+-induced+relaxation+of+rat+mesenteric+arteries.">Cytochrome P-450 metabolites of 2-arachidonoylglycerol play a role in Ca2+-induced relaxation of rat mesenteric arteries.</a> Am. J. Physiol. Heart Circ. Physiol. 294, 2363-2370 (2008).</li><li>Chen, W., Bergsman, J. B., Wang, X., Gilkey, G., Pierpoint, C. R., Daniel, E. A., Awumey, E. M., Dauban, P., Dodd, R. H., Ruat, M., Smith, S. M. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Presynaptic+external+calcium+signaling+involves+the+calcium-sensing+receptor+in+neocortical+nerve+terminals.">Presynaptic external calcium signaling involves the calcium-sensing receptor in neocortical nerve terminals.</a> PloS One. 5, e8563-e8563 (2010).</li><li>Bukoski, R. D. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=The+perivascular+sensory+nerve+Ca2++receptor+and+blood+pressure+regulation:+a+hypothesis.">The perivascular sensory nerve Ca2+ receptor and blood pressure regulation: a hypothesis.</a> Am. J. Hypertens. 11, 1117-1123 (1998).</li><li>Bukoski, R. D. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Dietary+Ca2++and+blood+pressure:+evidence+that+Ca2+-sensing+receptor+activated+sensory+nerve+dilator+activity+couples+changes+in+interstitial+Ca2++with+vascular.">Dietary Ca2+ and blood pressure: evidence that Ca2+-sensing receptor activated sensory nerve dilator activity couples changes in interstitial Ca2+ with vascular.</a> 16, 218-221 (2001).</li></ol>

Гипертония является одной из основных причин сердечно-сосудистых, церебральных и почечных заболеваемости / смертности. Возникновения гипертонии высок среди населения и соль-чувствительной гипертонии особенно высока в старение населения, и более распространенным в черных, чем белых. ...

<table border="1"><tbody><tr><th> Наименование оборудования / реагента </th><th> Компания </th><th> Номер в каталоге </th><th> Комментарии (необязательно) </th></tr><tr><td> Авто Двойной Myograph проводная система-510A DMT-США, Inc </td><td> Атланта, штат Джорджия. </td><td> 100151 </td><td></td></tr><tr><td> PowerLab/4SP системы сбора данных </td><td> ADInstruments, Колорадо-Спрингс, CO </td><td> ML750 </td><td> Новые модели с 4-16 входных каналов имеются. </td></tr><tr><td> Dell Dimension XPS Gen 4 Компьютер </td><td> Лощина </td><td></td><td></td></tr><tr><td> Stemi С. В. II (APO) Препарирование микроскоп с Глазные </td><td> Carl Zeiss International. </td><td></td><td></td></tr><tr><td> Вистар, Даль соль-чувствительной, Даль солеустойчивые крыс </td><td> Харлан Sprague Dawley, Индианаполис, IN. </td><td></td><td></td></tr><tr><td> Грызун чау </td><td> Харланд Tekland, Мэдисон, Висконсин. </td><td></td><td></td></tr><tr><td> Фенилефрин </td><td> Sigma-Aldrich, Сент-Луис, Миссури. </td><td></td><td></td></tr><tr><td> Другие химикаты </td><td></td><td></td><td> Все химические вещества, используемые были чистейшей классы имеются в продаже. </td></tr></tbody></table>

mesenteric artery contraction and relaxation studies using automated wire myography

Proximal resistance vessels, such as the mesenteric arteries, contribute substantially to the peripheral resistance. These small vessels of between 100-400 &mu;m in diameter function primarily in directing blood flow to various organs according to the overall requirements of the body. The rat mesenteric artery has a diameter greater than 100 &mu;m. The myography technique, first described by Mulvay and Halpern1, was based on the method proposed by Bevan and Osher2. The technique provides information about small vessels under isometric conditions, where substantial shortening of the muscle preparation is prevented. Since force production and sensitivity of vessels to different agonists is dependent on the extent of stretch, according to active tension-length relation, it is essential to conduct contraction studies under isometric conditions to prevent compliance of the mounting wires. Stainless steel wires are preferred to tungsten wires because of oxidation of the latter, which affects recorded responses3.The technique allows for the comparison of agonist-induced contractions of mounted vessels to obtain evidence for normal function of vascular smooth muscle cell receptors.
We have shown in several studies that isolated mesenteric arteries that are contracted with phenylyephrine relax upon addition of cumulative concentrations of extracellular calcium (Ca2+e). The findings led us to conclude that perivascular sensory nerves, which express the G protein-coupled Ca2+-sensing receptor (CaR), mediate this vasorelaxation response. Using an automated wire myography method, we show here that mesenteric arteries from Wistar, Dahl salt-sensitive(DS) and Dahl salt-resistant (DR) rats respond differently to Ca2+e. Tissues from Wistar rats showed higher Ca2+-sensitivity compared to those from DR and DS. Reduced CaR expression in mesenteric arteries from DS rats correlates with reduced Ca2+e-induced relaxation of isolated, pre-contracted arteries. The data suggest that the CaR is required for relaxation of mesenteric arteries under increased adrenergic tone, as occurs in hypertension, and indicate an inherent defect in the CaR signaling pathway in Dahl animals, which is much more severe in DS.
The method is useful in determining vascular reactivity ex vivo in mesenteric resistance arteries and similar small blood vessels and comparisons between different agonists and/or antagonists can be easily and consistently assessed side-by-side6,7,8.

Watch this Scientific Journal Video about Mesenteric Artery Contraction and Relaxation Studies Using Automated Wire Myography at JoVE.com

Mesenteric Artery Contraction and Relaxation Studies Using Automated Wire Myography

Автоматизированный метод миография для измерения силы в изолированных артерий брыжеечной описано. В нем занято Mulvany-Гальперн Авто Двойной Myograph провода 510A для определения ответов на фенилэфрин и внеклеточного кальция. Метод позволяет последовательно определения изометрической ответы на агонисты в мелких сосудах диаметром 60 - 300 мкм, независимо друг от друга.

Брыжеечной артерии Сокращение и отдых Исследования с помощью автоматизированной миография провода

Proximal resistance vessels, such as the mesenteric arteries, contribute substantially to the peripheral resistance. These small vessels of between ...

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