Name: tracking morphogenetic tissue deformations in the early chick embryo
Uploaded: 2011-10-17T12:00:00
Description: Watch this Scientific Journal Video about Tracking Morphogenetic Tissue Deformations in the Early Chick Embryo at JoVE.com

这项工作是由国立卫生研究院拨款R01 GM075200和R01 HL083393（LAT）的支持。我们承认从NIH T90的DA022871和Mallinckrodt放射研究所曝气生物滤池的奖学金支持，并从美国心脏协会09PRE2060795授予VDV值。

1。一般实验准备<ol><li>准备在层流罩组织培养基。 <ol><li>稀贝科改良Eagle培养基（DMEM）（1大号瓶4.5克/ L葡萄糖，碳酸氢钠，和L -谷氨酰胺）。加入10 mL青霉素/链霉素/新霉素抗生素。 </li><li>取出用无菌移液管的DMEM 100毫升和100毫升鸡血清取代。 </li><li>分装DMEM/10％的雏鸡血清/ 1％抗生素无菌的15 mL锥形瓶和冻结。 </li></ol></li><li>准备磷酸盐缓冲液（PBS）补充钙和镁。混合100毫升10X PBS中，去离子水9...

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前OVO文化早期鸡胚两种组织标签技术。首先使用通过铁磁性颗粒的亲脂性荧光染料，同时标签数百个细胞。然而，这种方法是目前不兼容光学相干断层扫描，荧光染料一般使用10月10日从周围组织的对比度很小。因此，我们使用聚苯乙烯微球标记时间推移华侨城分析组织的一种替代技术。这种技术产生的3 - D数据集，但必须小心不逐出组织的珠子。在适当的实验环境中使用这些方法?...

<table border="1"><table border="1"><tbody><tr><td> 材料/试剂 </td><td> 公司 </td><td> 目录编号 </td><td> 评论 </td></tr><tr><td> DMEM培养液 - 高血糖</td><td> Sigma - Aldrich公司</td><td> D5796 </td><td></td></tr><tr><td>青霉素/链霉素/新霉素</td><td> Sigma - Aldrich公司</td><td> P4083 </td><td></td></tr><tr><td>鸡血清</td><td> Invitrogen公司</td><td> 16110-082 </td><td></td></tr><tr><td>贝科的磷酸盐缓冲液</td><td> Sigma - Aldrich公司</td><td> D1408 </td><td> 10X </td></tr><tr><td>用Whatman＃2滤纸</td><td>瓦特曼</td><td> 1002 090 </td><td> 90毫米直径</td></tr><tr><td>玻璃微量移液器</td><td>世界精密德州仪器（WPI） </td><td> TW150 - 6 </td><td> 1.5毫米内层直径</td></tr><tr><td> DII </td><td> Invitrogen公司</td><td> D - 282 </td><td></td></tr><tr><td>铁减少</td><td> Mallinckrodt贝克公司</td><td> 5320 </td><td></td></tr><tr><td> 10微米的微球直径（黑色） </td><td> Polysciences公司</td><td> 24294 </td><td></td></tr><tr><td>三角洲T盘（时间的推移文化） </td><td> Bioptechs </td><td> 04200415B </td><td> 0.17毫米厚，黑色</td></tr><tr><td>三角洲T4培养皿控制器</td><td> Bioptechs </td><td> 0420-4-03 </td><td></td></tr><tr><td>微型泵变流量设备</td><td> Fisher Scientific则</td><td></td><td></td></tr></tbody></table>

tracking morphogenetic tissue deformations in the early chick embryo

胚胎上皮细胞进行复杂的变形（如弯曲，扭曲，折叠和拉伸），形成早期胚胎的原始器官。跟踪基准对这些细胞的表表面标记是一个估算，如生长，收缩和剪切形态数量以及建立的方法。然而，并非所有的表面标记技术容易适应传统的成像方式，具有不同的优势和局限性。在这里，我们描述了两种标记方法，并说明每种技术的效用。在第一种方法，荧光标记的数百个应用同时使用铁磁性颗粒的胚胎。然后，这些标签使用量的2 - D组织变形过程中的形态发生。在第二种方法中，聚苯乙烯微球用于非侵入性光学相干断层扫描（OCT）成像造影剂跟踪的3 - D的组织变形。这些技术已成功LY落实在我们的实验室studythe初头倍，心脏和大脑发育，物理机制，并应适应范围广的形态发生过程。

Watch this Scientific Journal Video about Tracking Morphogenetic Tissue Deformations in the Early Chick Embryo at JoVE.com

Tracking Morphogenetic Tissue Deformations in the Early Chick Embryo

本文介绍了表面的标签和<em&gt;前OVO</em&gt;在早期鸡胚组织培养。时间推移明场，荧光，和光学相干断层扫描成像技术适合。高时空分辨率的跟踪表面标签，使运动的数量，如在二维和三维计算的形态株（变形）。

在早期鸡胚跟踪形态组织变形

Embryonic epithelia undergo complex deformations (e.g. bending, twisting, folding, and stretching) to form the primitive organs of the early embryo. ...

Research

JoVE Journal

Biology

Method for Culture of Early Chick Embryos ex vivo (New Culture)

Method for Culture of Early Chick Embryos ex vivo New Culture

文化早期鸡胚体外（新文化）的方法

The chick embryo is a valuable tool in the study of early embryonic development. Its transparency, accessibility and ease of manipulation, make it an ...

科研

生物学

Double Whole Mount in situ Hybridization of Early Chick Embryos

双整装原位杂交技术在早期鸡胚

Assay for Neural Induction in the Chick Embryo

在鸡胚神经诱导的含量为

Electrophysiological Recording in the Drosophila Embryo

Electrophysiological Recording in the Drosophila Embryo

在电生理记录果蝇胚胎

Drosophila is a premier genetic model for the study of both embryonic development and functional neuroscience. Traditionally, these fields are quite ...

Quantitation of &gamma;H2AX Foci in Tissue Samples

Quantitation of ÃƒÅ½Ã‚Â³H2AX Foci in Tissue Samples

定量组织样本中的γH2AX灶

DNA double-strand breaks (DSBs) are particularly lethal and genotoxic lesions, that can arise either by endogenous (physiological or pathological) ...

Single Cell Transfection in Chick Embryos

单细胞转染鸡胚

A central theme in developmental biology is the diversification of lineages and the elucidation of underlying molecular mechanisms. This entails a ...

Chromatin Immunoprecipitation Assay for Tissue-specific Genes using Early-stage Mouse Embryos

使用早期的小鼠胚胎组织特异性基因的染色质免疫沉淀检测

Chromatin immunoprecipitation (ChIP) is a powerful tool to identify protein:chromatin interactions that occur in the context of living cells 1-3. This ...

Time-lapse Microscopy of Early Embryogenesis in Caenorhabditis elegans

Time-lapse Microscopy of Early Embryogenesis in Caenorhabditis elegans

时间推移显微镜早期胚胎发育线虫

Caenorhabditis elegans has often been used as a model system in studies of early developmental processes.  The transparency of the 
embryos, the genetic ...

In Ovo Electroporation in Embryonic Chick Retina

In Ovo Electroporation in Embryonic Chick Retina

在OVO在鸡胚视网膜电穿孔

Chicken embryonic retina is an excellent tool to study retinal development in higher vertebrates. Because of large size and external development, it is ...

Mouse Embryonic Development in a Serum-free Whole Embryo Culture System

鼠胚体外发育无血清全胚胎培养系统

Mid-gestation stage mouse embryos were cultured utilizing a serum-free culture medium prepared from commercially available stem cell media supplements in ...