Name: intravitreous injection for establishing ocular diseases model
Uploaded: 2007-10-01T12:45:27
Description: Watch this Scientific Journal Video about Intravitreous Injection for Establishing Ocular Diseases Model at JoVE.com

<p>青光眼在本实验室的研究是国家青光眼研究从美国卫生署助理基金会的支持。 </p>

<ol><li>准备使用移液器拉马的玻璃吸液管，连接到1ml注射器，并用封口膜密封连接。退回到吸液管的尖端解决方案2毫升，并准备注射。 </li><li>麻醉大鼠腹腔注射氯胺酮（80mg/kg）和甲苯噻嗪（8mg/kg）（体积比为2:1）。 </li><li>应用一个前玻璃体内注射局部麻醉剂对大鼠的眼睛，下降0.5％alcaine。 </li><li>大鼠的位置，并揭露鼻地区优越的眼睛。 </li><li>使用30号针头穿刺杆扁平疣水平优于鼻巩膜。避免接触眼部肌肉和血?...

<ol>
	<li>Chiu, K., Lam, T. T., Ying Ku, W. W., Caprioli, J., Kwong Kwong, J. M. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Calpain+and+N-methyl-d-aspartate+(NMDA)-induced+excitotoxicity+in+rat+retinas.">Calpain and N-methyl-d-aspartate (NMDA)-induced excitotoxicity in rat retinas.</a> <em style='font-style: italic'>Brain Research</em>. <b>1046</b>, 207-215 (2005).</li><li>Fisher, D., Pavlidis, M., Thanos, S. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Cataractogenic+lens+injury+prevents+traumatic+ganglion+cell+death+and+promotes+axonal+regeneration+both+in+vivo+and+in+culture.">Cataractogenic lens injury prevents traumatic ganglion cell death and promotes axonal regeneration both in vivo and in culture.</a> <em style='font-style: italic'>Investigative Ophthalmology &amp; Visual Science</em>. <b>41</b>, 3943-3954 (2000).</li><li>Ji, J. -. Z., Elyaman, W., Yip, H. K., Lee, V. W. H., Yick, L. -. W., Hugon, J., So, K. -. F. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=CNTF+promotes+survival+of+retinal+ganglion+cells+after+induction+of+ocular+hypertension+in+rats:+the+possible+involvement+of+STAT3+pathway.">CNTF promotes survival of retinal ganglion cells after induction of ocular hypertension in rats: the possible involvement of STAT3 pathway.</a> <em style='font-style: italic'>European Journal of Neuroscience</em>. <b>19</b>, 265-272 (2004).</li><li>Lam, T. T., Abler, A. S., Kwong, J. M. K., Tso, M. O. M. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=N-Methyl-D-Aspartate+(NMDA)-Induced+Apoptosis+in+Rat+Retina.">N-Methyl-D-Aspartate (NMDA)-Induced Apoptosis in Rat Retina.</a> <em style='font-style: italic'>Investigative Ophthalmology &amp; Visual Science</em>. <b>40</b>, 2391-2397 (1999).</li></ol>

<p>良好的玻璃体内注射液是用于建立动物模型以及直接人工处理。首先，注射部位不应该破坏视网膜结构。二，出血，应避免使用，以减少感染的风险。第三，镜片应不变，以避免外伤性白内障。最重要的是正常的眼部结构，以减少中断。 </p>

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		<th>Material Name</th>
		<th>Type</th>
		<th>Company</th>
		<th>Catalogue Number</th>
		<th>Comment</th>
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<tr>
<td>Operating Microscope </td>
<td>&nbsp;</td>
<td>Olympus</td>
<td>OME</td>
<td>&nbsp;</td>
<tr>
<td>Glass Pipettes </td>
<td>&nbsp;</td>
<td>World Precision Instruments, Inc.</td>
<td>&nbsp;</td>
<td>&nbsp;</td>
<tr>
<td>Pipette Puller </td>
<td>&nbsp;</td>
<td>David Kopf Instruments</td>
<td>&nbsp;</td>
<td>&nbsp;</td>
<tr>
<td>1ml syringes</td>
<td>&nbsp;</td>
<td>&nbsp;</td>
<td>&nbsp;</td>
<td>single use</td>
</tr>
<tr>
<td>30G needles</td>
<td>&nbsp;</td>
<td>&nbsp;</td>
<td>&nbsp;</td>
<td>single use</td>
</tr>
<tr>
<td>Ketamine</td>
<td>&nbsp;</td>
<td>Aldasan</td>
<td>&nbsp;</td>
<td>anesthetic</td>
</tr>
<tr>
<td>Xylazine</td>
<td>&nbsp;</td>
<td>Aldasan</td>
<td>&nbsp;</td>
<td>anesthetic</td>
</tr>
<tr>
<td>Alcaine</td>
<td>&nbsp;</td>
<td>Alcon-Couvreur</td>
<td>&nbsp;</td>
<td>0.5% proparacaine hydrochloride</td>
</tr>
<tr>
<td>Tobrex</td>
<td>&nbsp;</td>
<td>Alcon-Couvreur</td>
<td>&nbsp;</td>
<td>ointment, 3% tobramyxin.</td>
</tr>		</tbody>
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intravitreous injection for establishing ocular diseases model

<p class="jove_content">玻璃体内注射液是一种广泛使用的技术在视觉科学的研究。它可用于建立与眼部疾病或局部治疗的直接应用的动物模型。本视频介绍了如何使用简单和便宜的工具来完成的玻璃体内注射过程。使用1毫升注射器，而不是一个hemilton注射器，使用。实用技巧，如何作出适当的注射针头，用玻璃移液管以完美的技巧，以及如何轻松地连接用玻璃吸管将注射器针头紧紧地握在一起。 </p><p class="jove_content">要进行一个很好的的玻璃体内注射，有三个方面来观察：1）注射部位不应该破坏视网膜结构; 2）出血应避免，以减少感染的风险; 3）镜头应不变，以避免外伤性白内障。简单地说，最重要的一点是正常的眼部结构，以减少中断。为了避免中断视网膜，被选为优越的鼠眼的鼻地区。另外，在标准杆的平面，这是从鼠眼的角膜缘地区约1.5 mm针的穿刺点。轻轻推少量的玻璃体通过穿刺孔，以减少注射前的眼压。喷射角度45度，这是不太可能导致在大鼠眼外伤性白内障，从而避免了从透镜因素相关的并发症和影响力。在这次行动中，有没有切割，无出血，结膜和眼部肌肉。快速和轻微的损伤，一个成功的玻璃体内注射可以在几分钟内完成。 </p><p class="jove_content">在这个特殊的协议概述注入玻璃体内注射的具体。然而，这里介绍的方法和材料也可以用于其他注射给药程序的小型哺乳动物的脑，脊髓或其他器官。 </p>

Watch this Scientific Journal Video about Intravitreous Injection for Establishing Ocular Diseases Model at JoVE.com

Intravitreous Injection for Establishing Ocular Diseases Model

<p class="jove_content">玻璃体内注射液是一种视觉科学的研究，广泛用于眼部疾病或局部治疗的直接应用的技术。该视频展示了玻璃体内注射用1ml注射器与玻璃吸管的协议。有用的技巧，如何避免大出血和晶状体损伤。</p>

玻璃体内注射眼部疾病模型建立

Intravitreous injection is a widely used technique in visual sciences research. It can be used to establish animal models with ocular diseases or as ...

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