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DOI :
10.3791/3138-v
•
12:35 min
November 10th, 2011
Chapters
0:05
Title
2:37
Purification of Hepatocytes and SECs from Rat Liver
4:18
Internalization of 125I-SA-b-Hep or Other Suitable Labeled Ligand
6:55
Hepatocyte and Sinusoidal Endothelial Cell (SEC) Purification
12:06
Conclusion
11:08
Distribution of Biotin-Labeled Heparin
从动物获得的无细胞系存在的主要细胞,肝窦内皮细胞(秒)的研究必须执行。这种方法依赖于肝的消化和差速离心SEC随后的培养和实验净化。
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