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DOI :
10.3791/3139-v
•
8:29 min
October 2nd, 2011
Chapters
0:05
Title
1:19
Collection, Culturing and Staging Pupae
2:33
Dissection
3:40
Cleaning, Fixation and Primary Antibody Incubation
5:38
Secondary Antibody Incubation and Mounting
7:24
Images of Prepared Samples
8:00
Conclusion
の抗体染色ショウジョウバエ蛹は成人腹部発生遺伝学の遺伝学的解析を強化することができます。我々は段階の解剖、固定し、抗体染色のために我々のプロトコルを提示ショウジョウバエ蛹の腹部。
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