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DOI :
10.3791/3148-v
•
14:39 min
November 28th, 2011
Chapters
0:05
Title
3:34
Generating Conditioned Medium (CM) For the Colony Assay
5:13
Obtaining a Single Cell Suspension of Pancreatic-like Progenitors by Fluorescence Activated Cell Sorting
7:55
Plating Sorted Cells to Semi-solid Culture
10:49
Observing Colony Formation under the Light Microscope
11:47
Ngn3-EGFP+ Derived Colonies Express Insulin
13:21
Conclusion
描述一个立体的克隆形成实验,让胰祖细胞分化为胰岛素表达殖民地。此方法采用半固体甲基纤维素,基质胶和生长因子的媒体的优势,其中单祖细胞增殖和分化在体外,允许量化人口的功能祖。
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