Name: cd4+ t-lymphocyte capture using a disposable microfluidic chip for hiv
Uploaded: 2007-10-01T13:03:52
Description: Watch this Scientific Journal Video about CD4+ T-Lymphocyte Capture Using a Disposable Microfluidic Chip for HIV at JoVE.com

<ol style=";text-align:right;direction:rtl"><li style=";text-align:right;direction:rtl"> تعد قناة ميكروفلويديك مع سطح CD4 <sup>+</sup> المضادة للbiotinlayted. </li><li style=";text-align:right;direction:rtl"> ضخ 10 مليلتر من الدم في العينة 5 مل / دقيقة في قناة ميكروفلويديك مع ضخ حقنة. </li><li style=";text-align:right;direction:rtl"> اخراج الدم في 20 مل / دقيقة لمدة 20 دقيقة مع برنامج تلفزيوني. </li><li style=";text...

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		<thead>
		<tr>
		<th>Material Name</th>
		<th>Type</th>
		<th>Company</th>
		<th>Catalogue Number</th>
		<th>Comment</th>
		</tr>
		</thead>
		<tbody>
		
<tr>
<td>PDMS microchannel</td>
<td>&nbsp;</td>
<td>&nbsp;</td>
<td>&nbsp;</td>
<td>Fabricated by SU-8 mold</td>
</tr>
<tr>
<td>Blood</td>
<td>&nbsp;</td>
<td>&nbsp;</td>
<td>&nbsp;</td>
<td>Donated blood from normal human subject. Mass General Hospital, 10 X high density WBC or Brigham and Women&#x2019;s Hospital.</td>
</tr>
<tr>
<td>PBS</td>
<td>Buffer</td>
<td>Invitrogen</td>
<td>14190</td>
<td>1x Dulbecco&#x2019;s Phosphated Buffered Saline.</td>
</tr>
<tr>
<td>FACS lysing solution</td>
<td>&nbsp;</td>
<td>Becton Dickinson</td>
<td>349202</td>
<td>Diluted to 10% from stock solution with PBS.</td>
</tr>
<tr>
<td>Anti-CD4+ biotinlayted Ab</td>
<td>&nbsp;</td>
<td>Beckman Coulter</td>
<td>IM0704</td>
<td>Surface prepared with Anti-CD4+ biotinlayted Ab, diluted to 10% from stock solution with PBS/BSA after mixing with 0.2mg stock and 1ml deionized water. </td>
</tr>
<tr>
<td>goat anti-hCD3delta</td>
<td>pAB</td>
<td>Santa Cruz Biotechnology</td>
<td>sc-1128</td>
<td>Diluted to 10% from stock solution with PBS</td>
</tr>
<tr>
<td>rabbit anti-goat IgG PE</td>
<td>&nbsp;</td>
<td>Santa Cruz Biotechnology</td>
<td>sc-3755</td>
<td>Secondary Ab fro anti-CD3 Ab, diluted to 10% from stock solution with PBS</td>
</tr>
<tr>
<td>anti-human CD4+</td>
<td>&nbsp;</td>
<td>Becton Dickinson</td>
<td>557695</td>
<td>Alexa Fluor 488 labeled, diluted to 20% from stock solution with PBS. </td>
</tr>
<tr>
<td>DAPI</td>
<td>&nbsp;</td>
<td>Invitrogen</td>
<td>D1306</td>
<td>4&#x2019;,6-dianidino-2-phenylindole, dihydrochloride solution, diluted to 0.2% from stock solution with PBS.</td>
</tr>		</tbody>
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cd4+ t-lymphocyte capture using a disposable microfluidic chip for hiv

Watch this Scientific Journal Video about CD4+ T-Lymphocyte Capture Using a Disposable Microfluidic Chip for HIV at JoVE.com

CD4+ T-Lymphocyte Capture Using a Disposable Microfluidic Chip for HIV

CD4 + T - اللمفاويات التقاط باستخدام رقاقة ميكروفلويديك المتاح لفيروس نقص المناعة البشرية

Research

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Biology

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 The fabrication and operation of a gradient-generating microfluidic device for studying cellular behavior is described.  A microfluidic platform is an ...

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Laser capture microscopy, also known as laser microdissection (LMD), enables the user to isolate small numbers of cells or tissues from frozen or ...

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In this interview, Dr. Klapperich discusses the fabrication of thermoplastic microfluidic devices and their application for development of new ...

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While microfluidic technology is reaching a new level of maturity for macromolecular assays, cell-based assays are still at an infant stage1. This is ...

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HIV-1 integrates into the host chromosome of infected cells and persists as a provirus flanked by long terminal repeats. Current treatment strategies ...

Visualizing Antigen Specific CD4+ T Cells using MHC Class II Tetramers

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Major histocompatibility complex (MHC) class II tetramers allow the direct visualization of antigen specific CD4+ T cells by flow cytometry.  This method ...

The Microfluidic Probe: Operation and Use for Localized Surface Processing

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Microfluidic devices allow assays to be performed using minute amounts of sample and have recently been used to control the microenvironment of cells. ...

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Platelet aggregation occurs in response to vascular injury where the extracellular matrix below the endothelium has been exposed. The platelet adhesion ...

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Chemotaxis allows bacteria to approach sources of attractant chemicals or to avoid sources of repellent chemicals. Bacteria constantly monitor the ...