Name: cd4+ t-lymphocyte capture using a disposable microfluidic chip for hiv
Uploaded: 2007-10-01T13:03:52
Description: Watch this Scientific Journal Video about CD4+ T-Lymphocyte Capture Using a Disposable Microfluidic Chip for HIV at JoVE.com

<ol><li> 안티 CD4 <sup>+</sup> biotinlayted 표면 microfluidic 채널을 준비합니다. </li><li> 주사기 펌프와 microfluidic 채널에 5 ML / 분 샘플 혈액의 10 ML을 주사. </li><li> PBS로 20 분 20 ML / 분 혈액을 밖으로 플러시. </li><li> 15 분 5ul/min에 희석 외과 lysing 솔루션 (PBS로 재고 솔루션에서 10 % 희석) 주사. </li><li> 15 분 알을 품다. </li><li> 5 ML / 20 분 분 주요 인 경우에는 3 번 CD <sup>+</sup> 항체 솔루션 (PBS로 재고 솔루션에서 10 % 희석) 주사. </li><...

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		<thead>
		<tr>
		<th>Material Name</th>
		<th>Type</th>
		<th>Company</th>
		<th>Catalogue Number</th>
		<th>Comment</th>
		</tr>
		</thead>
		<tbody>
		
<tr>
<td>PDMS microchannel</td>
<td>&nbsp;</td>
<td>&nbsp;</td>
<td>&nbsp;</td>
<td>Fabricated by SU-8 mold</td>
</tr>
<tr>
<td>Blood</td>
<td>&nbsp;</td>
<td>&nbsp;</td>
<td>&nbsp;</td>
<td>Donated blood from normal human subject. Mass General Hospital, 10 X high density WBC or Brigham and Women&#x2019;s Hospital.</td>
</tr>
<tr>
<td>PBS</td>
<td>Buffer</td>
<td>Invitrogen</td>
<td>14190</td>
<td>1x Dulbecco&#x2019;s Phosphated Buffered Saline.</td>
</tr>
<tr>
<td>FACS lysing solution</td>
<td>&nbsp;</td>
<td>Becton Dickinson</td>
<td>349202</td>
<td>Diluted to 10% from stock solution with PBS.</td>
</tr>
<tr>
<td>Anti-CD4+ biotinlayted Ab</td>
<td>&nbsp;</td>
<td>Beckman Coulter</td>
<td>IM0704</td>
<td>Surface prepared with Anti-CD4+ biotinlayted Ab, diluted to 10% from stock solution with PBS/BSA after mixing with 0.2mg stock and 1ml deionized water. </td>
</tr>
<tr>
<td>goat anti-hCD3delta</td>
<td>pAB</td>
<td>Santa Cruz Biotechnology</td>
<td>sc-1128</td>
<td>Diluted to 10% from stock solution with PBS</td>
</tr>
<tr>
<td>rabbit anti-goat IgG PE</td>
<td>&nbsp;</td>
<td>Santa Cruz Biotechnology</td>
<td>sc-3755</td>
<td>Secondary Ab fro anti-CD3 Ab, diluted to 10% from stock solution with PBS</td>
</tr>
<tr>
<td>anti-human CD4+</td>
<td>&nbsp;</td>
<td>Becton Dickinson</td>
<td>557695</td>
<td>Alexa Fluor 488 labeled, diluted to 20% from stock solution with PBS. </td>
</tr>
<tr>
<td>DAPI</td>
<td>&nbsp;</td>
<td>Invitrogen</td>
<td>D1306</td>
<td>4&#x2019;,6-dianidino-2-phenylindole, dihydrochloride solution, diluted to 0.2% from stock solution with PBS.</td>
</tr>		</tbody>
		</table>
		</div>
	

cd4+ t-lymphocyte capture using a disposable microfluidic chip for hiv

Watch this Scientific Journal Video about CD4+ T-Lymphocyte Capture Using a Disposable Microfluidic Chip for HIV at JoVE.com

CD4+ T-Lymphocyte Capture Using a Disposable Microfluidic Chip for HIV

CD4 + HIV에 대한 일회용 Microfluidic 칩을 사용하여 T - 림프구 캡처

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