Name: recognition of epidermal transglutaminase by iga and tissue transglutaminase 2 antibodies in a rare case of rhesus dermatitis
Uploaded: 2011-12-15T12:00:00
Description: Watch this Scientific Journal Video about Recognition of Epidermal Transglutaminase by IgA and Tissue Transglutaminase 2 Antibodies in a Rare Case of Rhesus Dermatitis at JoVE.com

作者想感谢他们的技术援助阿曼达Tardo，博士穆斯塔法Bouljihad，斯蒂芬妮费力，卡罗尔柯尼博士艾琳Ribka，博士皮特迪迪埃和多萝西Kuebler。这项工作得到了NIH的拨款R01 - DK076653 DK076653 3R01 - 02S1金水TNPRC RR000164基地经营授予。

1。皮肤活检样品采集<ol><li>之前皮肤活检手术，麻醉动物肌注2.5毫克/公斤盐酸tiletamine zolazepam盐酸telazol的混合物（道奇堡动物健康，道奇堡，IA）。监控管理，直到斜卧麻醉的动物，然后删除其外壳。 </li><li>取下的头发和皮肤面积大小40刀片（奥斯特的专业产品，麦克明维尔，TN）利用奥斯特金A5单速兽医帆船利益无菌准备与优碘擦洗，酒精交替。一个在选定的活检部位的安全无菌孔的悬垂。 </...

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	<li>Diosdado, B., Wijmenga, C. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Molecular+mechanisms+of+the+adaptive,+innate+and+regulatory+immune+responses+in+the+intestinal+mucosa+of+celiac+disease+patients.">Molecular mechanisms of the adaptive, innate and regulatory immune responses in the intestinal mucosa of celiac disease patients.</a> Expert. Rev. Mol. Diagn. 5 (5), 681-700 (2005).</li><li>Schuppan, D., Dennis, M. D., Kelly, C. P. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Celiac+disease:+epidemiology,+pathogenesis,+diagnosis,+and+nutritional+management.">Celiac disease: epidemiology, pathogenesis, diagnosis, and nutritional management.</a> Nutr. Clin. Care. 8 (2), 54-69 (2005).</li><li>Sardy, M. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Epidermal+transglutaminase+(TGase+3)+is+the+autoantigen+of+dermatitis+herpetiformis.">Epidermal transglutaminase (TGase 3) is the autoantigen of dermatitis herpetiformis.</a> J. Exp. Med. 195 (6), 747-757 (2002).</li><li>Rose, C. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Autoantibodies+against+epidermal+transglutaminase+are+a+sensitive+diagnostic+marker+in+patients+with+dermatitis+herpetiformis+on+a+normal+or+gluten-free+diet.">Autoantibodies against epidermal transglutaminase are a sensitive diagnostic marker in patients with dermatitis herpetiformis on a normal or gluten-free diet.</a> J. Am. Acad. Dermatol. 61 (1), 39-43 (2009).</li><li>Bethune, M. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Transepithelial+transport+and+enzymatic+detoxification+of+gluten+in+gluten-sensitive+rhesus+macaques.">Transepithelial transport and enzymatic detoxification of gluten in gluten-sensitive rhesus macaques.</a> PLoS ONE. 3 (3), e1857-e1857 (2008).</li><li>Mazumdar, K. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Visualization+of+transepithelial+passage+of+the+immunogenic+33-residue+peptide+from+&#x03B1;-2+gliadin+in+gluten-sensitive+macaques.">Visualization of transepithelial passage of the immunogenic 33-residue peptide from &#x03B1;-2 gliadin in gluten-sensitive macaques.</a> PLoS ONE. 5 (4), e10228-e10228 (2010).</li><li>Schuppan, D., Junker, Y., Barisani, D. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Celiac+disease:+from+pathogenesis+to+novel+therapies.">Celiac disease: from pathogenesis to novel therapies.</a> Gastroenterol. 137 (6), 1912-1933 (2009).</li><li>Hunt, K. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Newly+identified+genetic+risk+variants+for+celiac+disease+related+to+the+immune+response.">Newly identified genetic risk variants for celiac disease related to the immune response.</a> Nature Genet. 40 (4), 395-402 (2008).</li><li>Abadie, V. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Integration+of+genetic+and+immunological+insights+into+a+model+of+celiac+disease+pathogenesis.">Integration of genetic and immunological insights into a model of celiac disease pathogenesis.</a> Annu. Rev. Immunol. 29, 493-525 (2011).</li></ol>

最近，我们新成立的非人类灵长类动物模型面筋敏感性是用于研究饮食面筋 5,6引发的肠道通透性变化。等离子体tTG2抗体以及肠道tTG2 + IGA +存款中检测到一些敏感的麸质的猕猴。由于我们最近认同在殖民地特发性皮炎的罕见事件恒河猴猕猴，这里没有通常的传染病/代谢的病因可能是成立，本研究的目的是，以评估如果tTG2 + IGA +抗体的存款可能被检测到的皮肤样本从这些罕见的情况下皮炎?...

<table border="1"><tbody><tr><td> 试剂或设备名称 </td><td> 公司 </td><td> 目录编号/产品编号 </td><td> 请注意 </td></tr><tr><td>抗人IGA - FITC </td><td> Sigma公司，圣路易斯，密苏里州</td><td> F - 5259 </td><td> 1:100工作液</td></tr><tr><td>反人权tTG2 </td><td> Thermo Scientific的卡拉马祖，心肌梗死</td><td> MS - 300 - P1ABX </td><td> 1:100工作液</td></tr><tr><td>抗鼠IgG1 ** </td><td> Invitrogen公司，卡尔斯巴德，CA </td><td> A - 21124 </td><td> 1:1,000工作液</td></tr><tr><td> TOPRO - 3 DNA探针*** </td><td> Invitrogen公司，卡尔斯巴德，CA </td><td> T - 3605 </td><td> 1:1,000工作液</td></tr><tr><td>锌福尔马林 （Z - FIX） </td><td> Anatech有限公司，巴特尔克里克，MI </td><td> 171 </td><td></td></tr><tr&gt; <td>华侨城冻结 中等</td><td>樱花Finetek，托伦斯，CA </td><td> 4583 </td><td></td></tr><tr><td> Cytoseal安装 中等</td><td>理查德艾伦科学，卡拉马祖，心肌梗死</td><td> 8312-4 </td><td></td></tr><tr><td>反淬火 解决方案</td><td> Sigma公司，圣路易斯，密苏里州</td><td> P 3130 </td><td></td></tr><tr><td> Ethilon （尼龙缝线） </td><td>爱惜康公司，强生公司，英国伯克郡， </td><td> 663G </td><td></td></tr><tr><td> Miltex打孔真皮活检仪器</td><td> Miltex，纽约，PA </td><td>不适用</td><td></td></tr><tr><td>奥斯特金A5单速度与规模的40刀片兽医快船</td><td>奥斯特产品，麦克明维尔，TN </td><td> 78005-050 </td><td></td></tr><tr><td>徕卡组织处理器</td><td>莱卡微系统公司布法罗树丛，KS </td><td> ASP300 </td><td></td></tr><tr><td>旋转式切片机</td><td> MICROM国际 华尔道夫，德国</td><td> HM325 </td><td></td></tr><tr><td>低温恒温器</td><td> Thermo Scientific的， 卡拉马祖MI </td><td> HM560 </td><td></td></tr><tr><td>共聚焦显微镜</td><td>莱卡，韦茨拉尔 德国</td><td> TCS SP2 </td><td></td></tr></tbody></table> *异硫氰酸荧光素 **中使用抗体后tTG2初级抗体（共轭的Alexa Fluor 568） **染料

recognition of epidermal transglutaminase by iga and tissue transglutaminase 2 antibodies in a rare case of rhesus dermatitis

组织转谷氨酰胺酶2（tTG2）是肠道的消化酶deamidates已经部分消化的饮食面筋如醇溶蛋白肽。在遗传倾向的个体，tTG2触发自身免疫反应的特点是由生产tTG2抗体和直接沉积到小肠壁 1,2 。这种抗体的存在，构成腹腔病（CD）的重要标志之一。表皮转谷氨酰胺酶（ETG），也可以作为在少数的CD患者的自身抗原的转谷氨酰胺酶家族的另一成员。在这些比较少见的情况下，ETG触发自身免疫反应（皮疹），临床上称为疱疹性皮炎（DH）。虽然CD和卫生署的发病的确切机制还不是很清楚，它是已知tTG2和血清抗体可以识别CD和卫生署病人 3,4 ETG份额抗原表位。 猕猴 ）皮炎（表1，图1和2）皮损活检标本用于研究受影响的组织。在一个动物（EM96）IgA和tTG2抗体（图3）光谱重叠证明。集中在深的表皮，真皮​​乳头周围，存在的双阳性tTG2 + IGA +细胞。这是符合卫生署病人描述的病变 3 。当EM96是一个无麸质饮食，皮炎，以及放置tTG2 + IGA +存款消失，不再检测到（图1-3）皮炎再现然而，基于EM96的饮食面筋（未显示）的重新引入。包括动物无关皮炎的其他猕猴，tTG2 + IGA +存款均未检出。无麸质饮食依赖性皮炎缓解EM96 tTG2存在+ IGA +在它的皮肤细胞表明自身免疫性疾病，卫生署状这种情况的发展机制。这是任何非人类灵长类动物中的DH -样皮炎的第一次报告。

Watch this Scientific Journal Video about Recognition of Epidermal Transglutaminase by IgA and Tissue Transglutaminase 2 Antibodies in a Rare Case of Rhesus Dermatitis at JoVE.com

Recognition of Epidermal Transglutaminase by IgA and Tissue Transglutaminase 2 Antibodies in a Rare Case of Rhesus Dermatitis

疱疹性皮炎（DH）是一种慢性炎症性IGA和表皮转谷氨酰胺酶（ETG）之间的自身免疫反应的条件。卫生署发展中的麸质敏感和/或腹腔的患者非常小的部分。这项研究的结果表明，卫生署也可以在idiopatic皮炎症状猕猴主机发展。

在极少数情况下，表皮转谷氨酰胺酶IgA和组织转谷氨酰胺酶2抗体的识别恒河猴皮炎

Tissue transglutaminase 2 (tTG2) is an intestinal digestive enzyme which deamidates already partially digested dietary gluten e.g. gliadin peptides. In ...

Research

JoVE Journal

Immunology and Infection

Recognition of Epidermal Transglutaminase by IgA and Tissue Transglutaminase 2 Antibodies in a Rare Case of Rhesus Dermatitis

Multicolor Flow Cytometry Analyses of Cellular Immune Response in Rhesus Macaques

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