Name: recognition of epidermal transglutaminase by iga and tissue transglutaminase 2 antibodies in a rare case of rhesus dermatitis
Uploaded: 2011-12-15T12:00:00
Description: Watch this Scientific Journal Video about Recognition of Epidermal Transglutaminase by IgA and Tissue Transglutaminase 2 Antibodies in a Rare Case of Rhesus Dermatitis at JoVE.com

Forfatterne vil gerne takke Amanda Tardo, Dr. Mostafa Bouljihad, Stephanie Feely, Carol Coyne, Dr. Erin Ribka, Dr. Pete Didier og Dorothy Kuebler for deres tekniske assistance. Dette arbejde blev støttet af NIH giver R01-DK076653 og 3R01-DK076653-02S1 til KS og bunden driftstilskud af TNPRC RR000164.

1. Hudbiopsi prøvetagning <ol><li> Forud for hudbiopsi procedure, bedøver dyr intramuskulært med 2,5 mg / kg af tiletamine hydrochlorid og zolazepam hydrochlorid telazol blanding (Fort Dodge Animal Health, Fort Dodge, IA). Overvåg dyr fra administration af bedøvelsesmiddel, indtil tilbagelænethed og derefter fjerne fra deres indhegning. </li><li> Fjern hår fra huden interesseområde anvendelse af en Oster Golden A5 Single Speed ​​Veterinary Clipper med en str. 40 klinge (Oster professionelle produkter, McMi...

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	<li>Diosdado, B., Wijmenga, C. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Molecular+mechanisms+of+the+adaptive,+innate+and+regulatory+immune+responses+in+the+intestinal+mucosa+of+celiac+disease+patients.">Molecular mechanisms of the adaptive, innate and regulatory immune responses in the intestinal mucosa of celiac disease patients.</a> Expert. Rev. Mol. Diagn. 5 (5), 681-700 (2005).</li><li>Schuppan, D., Dennis, M. D., Kelly, C. P. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Celiac+disease:+epidemiology,+pathogenesis,+diagnosis,+and+nutritional+management.">Celiac disease: epidemiology, pathogenesis, diagnosis, and nutritional management.</a> Nutr. Clin. Care. 8 (2), 54-69 (2005).</li><li>Sardy, M. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Epidermal+transglutaminase+(TGase+3)+is+the+autoantigen+of+dermatitis+herpetiformis.">Epidermal transglutaminase (TGase 3) is the autoantigen of dermatitis herpetiformis.</a> J. Exp. Med. 195 (6), 747-757 (2002).</li><li>Rose, C. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Autoantibodies+against+epidermal+transglutaminase+are+a+sensitive+diagnostic+marker+in+patients+with+dermatitis+herpetiformis+on+a+normal+or+gluten-free+diet.">Autoantibodies against epidermal transglutaminase are a sensitive diagnostic marker in patients with dermatitis herpetiformis on a normal or gluten-free diet.</a> J. Am. Acad. Dermatol. 61 (1), 39-43 (2009).</li><li>Bethune, M. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Transepithelial+transport+and+enzymatic+detoxification+of+gluten+in+gluten-sensitive+rhesus+macaques.">Transepithelial transport and enzymatic detoxification of gluten in gluten-sensitive rhesus macaques.</a> PLoS ONE. 3 (3), e1857-e1857 (2008).</li><li>Mazumdar, K. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Visualization+of+transepithelial+passage+of+the+immunogenic+33-residue+peptide+from+&#x03B1;-2+gliadin+in+gluten-sensitive+macaques.">Visualization of transepithelial passage of the immunogenic 33-residue peptide from &#x03B1;-2 gliadin in gluten-sensitive macaques.</a> PLoS ONE. 5 (4), e10228-e10228 (2010).</li><li>Schuppan, D., Junker, Y., Barisani, D. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Celiac+disease:+from+pathogenesis+to+novel+therapies.">Celiac disease: from pathogenesis to novel therapies.</a> Gastroenterol. 137 (6), 1912-1933 (2009).</li><li>Hunt, K. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Newly+identified+genetic+risk+variants+for+celiac+disease+related+to+the+immune+response.">Newly identified genetic risk variants for celiac disease related to the immune response.</a> Nature Genet. 40 (4), 395-402 (2008).</li><li>Abadie, V. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Integration+of+genetic+and+immunological+insights+into+a+model+of+celiac+disease+pathogenesis.">Integration of genetic and immunological insights into a model of celiac disease pathogenesis.</a> Annu. Rev. Immunol. 29, 493-525 (2011).</li></ol>

Vores nyetablerede ikke-menneskelig primat model af gluten følsomhed blev for nylig anvendt til at studere den intestinale permeabilitet ændringer der udløses af kosten gluten 5,6. Plasma tTG2 antistoffer samt intestinal tTG2 + IgA + indskud blev påvist i nogle gluten-følsomme makakaber. Da vi for nylig identificeret sjældne tilfælde af idiopatisk dermatitis i koloni rhesus makakaber, hvor der ikke plejer (smitsom / metabolisk) ætiologi kunne etableres, Formålet med denne undersøgelse var at vurdere...

<table border="1"><tbody><tr><td> Reagensets navn, eller udstyr </td><td> Firma </td><td> Katalognummer / Product Code </td><td> Bemærk </td></tr><tr><td> Anti-Human IgA-FITC * </td><td> Sigma, St. Louis, MO </td><td> F-5259 </td><td> 1:100 brugsfortynding </td></tr><tr><td> Anti-Human tTG2 </td><td> Thermo Scientific, Kalamazoo, MI </td><td> MS-300-P1ABX </td><td> 1:100 brugsfortynding </td></tr><tr><td> Anti-Mouse IgG1 ** </td><td> Invitrogen, Carlsbad, CA </td><td> A-21124 </td><td> 1:1,000 brugsfortynding </td></tr><tr><td> ToPro-3 DNA-probe *** </td><td> Invitrogen, Carlsbad, CA </td><td> T 3605 </td><td> 1:1,000 brugsfortynding </td></tr><tr><td> Zink Formalin (Z-fix) </td><td> Anatech Ltd, Battle Creek, MI </td><td> 171 </td><td></td></tr><tr&gt; <td> Oktober Frysning Medium </td><td> Sakura Finetek, Torrence, CA </td><td> 4583 </td><td></td></tr><tr><td> Cytoseal Montering Medium </td><td> Richard-Allan Videnskabelige, Kalamazoo, MI </td><td> 8312-4 </td><td></td></tr><tr><td> Anti-Quenching Løsning </td><td> Sigma, St. Louis, MO </td><td> P 3130 </td><td></td></tr><tr><td> Ethilon (Nylon sutur) </td><td> Ethicon, Johnson &amp; Johnson, Berkshire, Storbritannien </td><td> 663G </td><td></td></tr><tr><td> Miltex Punch Dermal Biopsi Instrument </td><td> Miltex, York, PA </td><td> NA </td><td></td></tr><tr><td> Oster Golden A5 Single Speed ​​Vet Clipper med en størrelse 40 klinge </td><td> Oster Products, McMinnville, TN </td><td> 78005-050 </td><td></td></tr><tr><td> Leica Tissue Processor </td><td> Leica Microsystems Inc., Buffalo Grove, KS </td><td> ASP300 </td><td></td></tr><tr><td> Rotary mikrotom </td><td> MicroM International, Waldorf, Tyskland </td><td> HM325 </td><td></td></tr><tr><td> Kryostat </td><td> Thermo Scientific, Kalamazoo, MI </td><td> HM560 </td><td></td></tr><tr><td> Konfokal mikroskop </td><td> Leica, Wetzlar Tyskland </td><td> TCS SP2 </td><td></td></tr></tbody></table> * Fluoresceinisothiocyanat ** Sekundær (Alexa Fluor 568-konjugeret) antistof anvendes efter tTG2 primære antistof ** Dye

recognition of epidermal transglutaminase by iga and tissue transglutaminase 2 antibodies in a rare case of rhesus dermatitis

Tissue transglutaminase 2 (tTG2) er en tarm fordøjelsesfremmende enzym, der deamidates allerede er delvist fordøjes kosten gluten f.eks gliadin peptider. I genetisk disponerede individer, udløser tTG2 autoimmune reaktioner, som er karakteriseret ved produktionen af tTG2 antistoffer og deres direkte deposition i tyndtarmens væg 1,2. Tilstedeværelsen af ​​sådanne antistoffer er en af ​​de vigtigste kendetegn for cøliaki (CD). Epidermal transglutaminase (ETG) er et andet medlem af transglutaminase familie, der også kan fungere som en autoantigen i en lille minoritet af CD patienterne. I disse relativt sjældne tilfælde, udløser ETG en autoimmun reaktion (hududslæt) er klinisk kendt som dermatitis herpetiformis (DH). Selvom den nøjagtige mekanisme af CD og DH patogenese ikke er godt forstået, er det kendt, at tTG2 og ETG aksjer antigene epitoper, der kan genkendes af serum antistoffer fra både CD og DH patienter 3,4. (Macaca mulatta) med dermatitis (Tabel 1, Fig. 1 og 2) blev brugt til at studere de ramte væv. I et dyr (EM96) en spektral overlapning af IgA og tTG2 antistoffer (Fig. 3) blev demonstreret. Tilstedeværelsen af ​​dobbelt-positive tTG2 + IgA + celler blev fokuseret i den dybe overhuden, omkring den dermale papiller. Dette er i overensstemmelse med læsioner, der er beskrevet i DH patienter på 3. Da EM96 blev placeret på en glutenfri kost, dermatitis, samt tTG2 + IgA + indskud forsvandt og blev ikke længere kan opfattes (fig. 1-3). Dermatitis genopstod dog baseret på genindførelse af kosten gluten i EM96 (ikke vist). I andre makakaber herunder dyr med uafhængige dermatitis, blev de tTG2 + IgA + indskud ikke fundet. Glutenfri kost-afhængige eftergivelse af dermatitis i EM96 sammen med tilstedeværelsen af ​​tTG2 + IgA + celler i sin hud tyder på en autoimmun, DH-lignendemekanisme for udvikling af denne tilstand. Dette er den første rapport af DH-lignende dermatitis i enhver ikke-menneskelige primater.

Watch this Scientific Journal Video about Recognition of Epidermal Transglutaminase by IgA and Tissue Transglutaminase 2 Antibodies in a Rare Case of Rhesus Dermatitis at JoVE.com

Recognition of Epidermal Transglutaminase by IgA and Tissue Transglutaminase 2 Antibodies in a Rare Case of Rhesus Dermatitis

Dermatitis herpetiformis (DH) er en kronisk inflammatorisk tilstand karakteriseret ved en autoimmun reaktion mellem IgA og epidermal transglutaminase (ETG). DH udvikler sig i en meget lille del af gluten-følsomme og / eller cøliaki patienter. Resultaterne af denne undersøgelse tyder på, at DH også kan udvikle sig i en rhesus abe vært med symptomer på idiopatisk dermatitis.

Anerkendelse af Epidermal transglutaminase ved IgA og væv transglutaminase 2 antistoffer i et sjældent tilfælde af Rhesus Dermatitis

Tissue transglutaminase 2 (tTG2) is an intestinal digestive enzyme which deamidates already partially digested dietary gluten e.g. gliadin peptides. In ...

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Immunology and Infection

Recognition of Epidermal Transglutaminase by IgA and Tissue Transglutaminase 2 Antibodies in a Rare Case of Rhesus Dermatitis

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