Name: a swine model of neonatal asphyxia
Uploaded: 2011-10-11T12:00:00
Description: Watch this Scientific Journal Video about A Swine Model of Neonatal Asphyxia at JoVE.com

作者想感谢加拿大医学研究，健康研究机构（MOP53116）和阿尔伯塔省遗产基金会的工作补助，并建立基金，分别支持发展的实验模型。

1。麻醉<ol><li>设置在2L/MIN麻醉机的流率。连接的排气真空抽吸。 </li><li>电荷与麻醉气体（异氟烷）在5％（〜3分钟）的面罩。 </li><li>新生仔猪将诱导与吸入异氟醚的5％，在100％的氧气（〜3分钟）。 </li><li>保持在2％至3％的异氟醚麻醉。经0.5％为适宜的异氟烷的精细调整，然而，它的范围可以从0.5到5％的条件取决于仔猪。 </li><li>一旦已经建立血管通路，吸入麻醉，可切换使用芬太尼（5-50微克/...

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	<li>Borke, W. B. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Increased+myocardial+matrix+metalloproteinases+in+hypoxic+newborn+pigs+during+resuscitation:+effects+of+oxygen+and+carbon+dioxide.">Increased myocardial matrix metalloproteinases in hypoxic newborn pigs during resuscitation: effects of oxygen and carbon dioxide.</a> Eur. J. Clin. Invest. 34, 459-466 (2004).</li><li>Munkeby, B. H. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Resuscitation+of+hypoxic+piglets+with+100%+O2+increases+pulmonary+metalloproteinases+and+IL-8.">Resuscitation of hypoxic piglets with 100% O2 increases pulmonary metalloproteinases and IL-8.</a> Pediatr. 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目前的实验协议有一个优势，在缺氧复氧过程中，检查全身和局部的血流动力学变化新生儿的主题。我们还可以检查用于改善心血管功能在恢复过程中各自的效果的干预。我们和其他人的经验和发现新生儿窒息，在心血管，肺，神经系统，胃肠道，肝5 6，肾，肾上腺7和血液系统的影响的研究报道。重要的是要了解心血管功能的连续的测量数据与信息的基础上，它在技术上...

a swine model of neonatal asphyxia

全球每年有超过100万新生儿死于窒息。窒息新生儿常见的有包括低血压，灌注赤字，缺氧缺血性脑病，肺部高血压，vasculopathic小肠结肠炎，肾功能衰竭和血栓栓塞并发症多器官功能衰竭。动物模型的开发，以帮助我们了解新生儿窒息的病理生理学和药理学。在啮齿动物和新生羔羊相比，新生仔猪已被证明是一个有价值的模型。新生仔猪有几个优势，包括类似的发展36-38周的人类胎儿具有可比性的身体系统，车身尺寸大（约1.5-2公斤出生），使仪器仪表和监测的动物和控制的混杂变量缺氧和血流动力学紊乱。 在这里，我们描述一个实验性协议，以模拟新生儿窒息，让我们检查系统·麦克风和区域的血流动力学变化窒息和复氧过程以及相应的干预效果。此外，该模型具有的优势，同时研究多器官功能衰竭或功能障碍和身体各个系统的相互作用。实验模型是一个非生存的过程，涉及到外科手术器械新生仔猪（1-3日龄和体重1.5-2.5公斤，混合品种），允许设立机械通气，血管（动脉和中央静脉）访问导管的位置不断在不同的动脉，包括主肺动脉，颈总动脉，肠系膜上动脉和左侧肾动脉血管内的压力和血流量监控和流量的探头（跨声速公司）。使用这些手术仪表仔猪30-60分钟，由Z &lt;10％的血流动力学参数的变化和正常的血液气体稳定后，我们开始了一个实验性的协议重度低氧血症是通过正常碳酸肺泡缺氧引起的。通风，有10-15％的氧气通过增加吸入氮气浓度为2h，旨在为动脉血氧饱和度为30％至40％，仔猪。这种程度的低氧血症会产生严重的代谢性酸中毒，全身性低血压和心源性休克，重要器官灌注不足的临床窒息。缺氧随后复氧与100％的氧气为0.5小时，然后21％的氧气为3.5小时。药物干预可以在适当的时候推出及其影响的调查，在一项双盲，随机块的方式。

Watch this Scientific Journal Video about A Swine Model of Neonatal Asphyxia at JoVE.com

A Swine Model of Neonatal Asphyxia

大动物模型具有良好的平移值在新生儿窒息的生理学和药理学考试。新生仔猪，，模拟新生儿窒息，有全身和局部的血流动力学，氧的运输，生化和病理的途径和相关研究的优势，我们开发了一个实验性协议。

新生儿窒息了猪模型

Annually more than 1 million neonates die worldwide as related to asphyxia. Asphyxiated neonates commonly have multi-organ failure including hypotension, ...

Research

JoVE Journal

Medicine

A Piglet Model of Neonatal Hypoxic-Ischemic Encephalopathy

新生儿缺氧缺血性脑病的小猪模型

Birth asphyxia, which causes hypoxic-ischemic encephalopathy (HIE), accounts for 0.66 million deaths worldwide each year, about a quarter of the ...

科研

医学

A Neonatal Mouse Spinal Cord Compression Injury Model

新生儿小鼠脊髓压迫损伤模型

Spinal cord injury (SCI) typically causes devastating neurological deficits, particularly through damage to fibers descending from the brain to the spinal ...

Myocardial Infarction in Neonatal Mice, A Model of Cardiac Regeneration

心肌梗死新生小鼠，心肌再生的模型

Myocardial infarction induced by coronary artery ligation has been used in many animal models as a tool to study the mechanisms of cardiac repair and ...

A Chronic Cardiac Ischemia Model in Swine Using an Ameroid Constrictor

应用 Ameroid 的猪慢性心肌缺血模型

Cardiovascular disease remains the number one cause of mortality in the United States. There are numerous approaches to treating these diseases, but ...

Tachycardia-Induced Cardiomyopathy As a Chronic Heart Failure Model in Swine

心动过速诱发的心肌病作为猪慢性心力衰竭模型的研究

A stable and reliable model of chronic heart failure is required for many experiments to understand hemodynamics or to test effects of new treatment ...

Severe Burn Injury in a Swine Model for Clinical Dressing Assessment

猪临床敷料评价模型中的严重烧伤

Wound healing is a dynamic repair process and is the most complex biological process in human life. In response to burn injury, alterations in biological ...

Establishing a Swine Model of Post-myocardial Infarction Heart Failure for Stem Cell Treatment

建立干细胞治疗心肌梗塞心力衰竭的猪模型

Although advances have been achieved in the treatment of heart failure (HF) following myocardial infarction (MI), HF following MI remains one of the major ...

Myocardial Infarction by Percutaneous Embolization Coil Deployment in a Swine Model

Myocardial infarction (MI) is the leading cause of mortality worldwide. Despite the use of evidence-based treatments, including coronary revascularization ...

A Neonatal BALB/c Mouse Model of Necrotizing Enterocolitis

Necrotizing enterocolitis (NEC) is the most severe gastrointestinal (GI) disease that often occurs in premature infants, especially very low birth weight ...

Effect of Hyaluronic Acid 35 kDa on an In Vitro Model of Preterm Small Intestinal Injury and Healing Using Enteroid-Derived Monolayers

Effect of Hyaluronic Acid 35 kDa on an In Vitro Model of Preterm Small Intestinal Injury and Healing Using Enteroid-Derived Monolayers

透明质酸35 kDa对肠源单层早产小肠损伤体 外 模型及愈合的影响

In vitro scratch wound assays are commonly used to investigate the mechanisms and characteristics of epithelial healing in a variety of tissue types. ...