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DOI :
10.3791/3170-v
•
12:34 min
October 21st, 2011
Chapters
0:05
Title
1:52
DNA Preparation and Denaturation
2:57
Bisulphite Deamination
6:38
PCR Amplification
9:08
Results of DNA Methylation Analysis
12:15
Conclusion
DNA甲基化分析的黄金标准是亚硫酸氢转换的DNA基因组测序。 5 - 甲基胞嘧啶(5 - MEC)亚硫酸氢在酸性条件下脱氨相比,这种方法需要利用胞嘧啶的敏感性增加。后PCR扩增目标基因组DNA甲基化的胞嘧啶甲基化的胞嘧啶可以区分。
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