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DOI :
10.3791/3170-v
•
12:34 min
October 21st, 2011
Chapters
0:05
Title
1:52
DNA Preparation and Denaturation
2:57
Bisulphite Deamination
6:38
PCR Amplification
9:08
Results of DNA Methylation Analysis
12:15
Conclusion
DNAメチル化解析のためのゴールドスタンダードは、重亜硫酸変換したDNAのゲノム配列決定です。このメソッドは、酸性条件下で脱アミノ化を亜硫酸、5 - メチルシトシン(5 - MEC)と比較しシトシンの感度の向上を活用しています。非メチル化シトシンを標的ゲノムDNAのPCR増幅後にメチル化シトシンを区別することができます。
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