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DOI :
10.3791/3171-v
•
11:08 min
April 8th, 2012
Chapters
0:05
Title
1:00
Intro
2:28
Background on Efficient Lentiviral Packaging
5:47
Concentrating the Viral Supernatant
3:31
Transfecting 293TN Cells with Packaging Plasmids and Expression Constructs
7:36
Transduction of HT1080 Control Cells for Viral Titering
9:21
Successful Transfection and Transduction
10:19
Conclusion
慢病毒表达载体的稳定表达不同的效应分子的分裂和非分裂哺乳动物细胞和整个生物体或记者构造最有效的车辆。在这里,我们提供了关于如何打包pseudoviral颗粒lentivector表达结构和转导靶细胞使用pseudoviral颗粒协议。
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