Name: lensless fluorescent microscopy on a chip
Uploaded: 2011-08-17T13:00:00
Description: Watch this Scientific Journal Video about Lensless Fluorescent Microscopy on a Chip at JoVE.com

A.奥兹坎感激地承认了国家科学基金会职业奖，羚羊青年研究者奖2009年和办公室的主任，国立卫生研究院国立卫生研究院主任的新的创新奖DP2OD006427的支持。作者也承认，比尔和梅林达盖茨基金会，沃达丰美洲基金会，和NSF BISH的程序（＃0754880和0930501奖）的支持。

在本节中，我们会审查我们的无透镜片上的荧光显微镜平台 1-4实验方法。为了证明这种技术的能力，我们将显示荧光微颗粒和标记的白血细胞片上的成像效果。虽然这里不讨论，也可以在同一lensfree荧光显微镜平台可以用来形象小动物模型，如转基因C 。 线虫样本3。 1。片上的无透镜成像平台的设计我们的无透镜片上的成像?...

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我们展示了一个芯片上的荧光显微镜平台，可以实现例如，&lt;4μm的空间分辨率比例如，&gt; 0.6-8厘米2没有使用任何镜头，机械扫描或薄膜干涉滤波器领域的视图。在这种技术中，使用了光纤面板或锥度，从对象的荧光收集，然后传递到如的CCD光电传感器阵列与二维阵列的光纤电缆/ CMOS芯片。这些收购的lensfree图像，然后迅速处理，产生超过4μm的决议&gt; 0.6-8厘米2，使用压缩采样/?...

<table border="1"><tbody><tr><th>材料名称</th><th>公司</th><th>目录编号</th></tr><tr><td>电荷耦合器件（CCD） </td><td>柯达</td><td> KAF - 8300 </td></tr><tr><td>电荷耦合器件（CCD） </td><td>柯达</td><td> KAF - 11002 </td></tr><tr><td>电荷耦合器件（CCD） </td><td>柯达</td><td> KAF - 39000 </td></tr><tr><td>互补金属氧化物半导体（CMOS） </td><td>美光科技公司</td><td> MT9T031C12STCD </td></tr><tr><td>高功率LED光源</td><td> Thorlabs </td><td> M455L2 - C2 </td></tr><tr><td>高功率LED驱动器</td><td> Thorlabs </td><td> LEDD1B </td></tr><tr><td>光纤耦合LED光源</td><td> Mightex </td><td> FCS - 0625 - 000 </td></tr><tr><td>真空笔</td><td>埃德蒙光学</td><td> NT57 - 636 </td></tr><tr><td> 2，4，10微米Fluospheres </td><td> Invitrogen公司</td><td> F - 8826，F - 8859，F - 8836 </td></tr><tr><td> RBS裂解液1X </td><td> eBioscience公司</td><td> 00-4333 </td></tr><tr><td>祥发16标签试剂</td><td> Invitrogen公司</td><td> S7578 </td></tr><tr><td>光纤面板</td><td>埃德蒙光学</td><td> NT55 - 142 </td></tr><tr><td>光纤光锥</td><td>埃德蒙光学</td><td> NT55 - 134 </td></tr><tr><td>棱镜</td><td>埃德蒙光学</td><td> NT47 - 626，NT45 - 403 </td></tr><tr><td>过滤器</td><td>埃德蒙光学</td><td> NT39 - 417 </td></tr><tr><td>硅橡胶弹性体</td><td>道康宁</td><td> Slygard 184 </td></tr></tbody></table>

lensless fluorescent microscopy on a chip

一般无透镜成像芯片，旨在更简单和更紧凑的设计，以取代笨重的镜头，尤其是对高通量筛选应用光学显微镜，。这个新兴的技术平台，以消除潜在需要的笨重和/或昂贵的光学元件，通过新颖的理论和数字重建算法的帮助。按照同样的思路，在这里我们展示了一个芯片上的荧光显微镜模式，可以实现超过超宽领域的视角（FOV）的4μm的空间分辨率&gt; 0.6-8厘米2例如，没有使用任何镜头，机械扫描或基于薄膜干涉滤光片。在这种技术中，荧光激发是通过由非相干源照明棱镜或半球形玻璃界面。与整个物体的体积相互作用后，这激发光全内反射（TIR）的过程，是发生在样品的微流体芯片的底部被拒绝。从激发对象的荧光是由光纤面板或锥度然后收集和交付作为一个电荷耦合器件（CCD）光电传感器阵列。通过使用压缩采样的解码算法，收购lensfree原料样品的荧光图像，可以迅速处理产量，例如，0.6-8厘米2 &lt;4μm的分辨率超过了视野。此外，垂直堆叠，如分离的微通道，50-100微米，也可以成功地使用相同的lensfree片上显微镜平台，这进一步增加了这种方式的整体吞吐量成像。这个紧凑的芯片上的荧光成像平台，它背后的快速压缩解码器，可用于高通量流式细胞仪，罕见的细胞研究和基因芯片分析是相当宝贵的。

Watch this Scientific Journal Video about Lensless Fluorescent Microscopy on a Chip at JoVE.com

Lensless Fluorescent Microscopy on a Chip

一个无透镜片上荧光显微镜平台，证明可以超过，如超宽现场，查看荧光图像对象，&gt; 0.6-8平方厘米&lt;4μm的使用压缩采样的解码算法的决议。这种紧凑型和宽视场荧光片上的成像方式，可用于高通量流式细胞仪，罕见的细胞研究和基因芯片分析的价值。

片上的无透镜荧光显微镜

On-chip lensless imaging in general aims to replace bulky lens-based optical microscopes with simpler and more compact designs, especially for ...

Research

JoVE Journal

Bioengineering

Lensfree On-chip Tomographic Microscopy Employing Multi-angle Illumination and Pixel Super-resolution

lensfree芯片断层用人多角度的光照和像素超高分辨率的显微镜

Tomographic imaging has been a widely used tool in medicine as it can provide three-dimensional (3D) structural information regarding objects of different ...

科研

生物工程

On-Chip Endothelial Inflammatory Phenotyping

片上内皮炎症表型

Atherogenesis is potentiated by metabolic abnormalities that contribute to a heightened state of systemic inflammation resulting in endothelial ...

Wide-field Fluorescent Microscopy and Fluorescent Imaging Flow Cytometry on a Cell-phone

一个手机上的宽视场荧光显微镜和荧光成像流式细胞仪

Fluorescent microscopy and flow cytometry are widely used tools in biomedical research and clinical diagnosis. However these devices are in general ...

Procedure for the Development of Multi-depth Circular Cross-sectional Endothelialized Microchannels-on-a-chip

发展多深的圆形横截面微内皮化的一个芯片的程序

Efforts have been focused on developing in vitro assays for the study of microvessels because in vivo animal studies are more time-consuming, expensive, ...

Stress-induced Antibiotic Susceptibility Testing on a Chip

芯片上的压力诱导抗生素易感性测试

We have developed a rapid microfluidic method for antibiotic susceptibility testing in a stress-based environment. Fluid is passed at high speeds over ...

A Novel Method for Localizing Reporter Fluorescent Beads Near the Cell Culture Surface for Traction Force Microscopy

新方法本地化记者荧光珠近细胞培养表面的牵引力显微镜

PA gels have long been used as a platform to study cell traction forces due to ease of fabrication and the ability to tune their elastic properties. When ...

PIP-on-a-chip: A Label-free Study of Protein-phosphoinositide Interactions

PIP-on-a-chip：蛋白质 - 磷酸肌醇相互作用的无标记研究

Numerous cellular proteins interact with membrane surfaces to affect essential cellular processes. These interactions can be directed towards a specific ...

Dry Film Photoresist-based Electrochemical Microfluidic Biosensor Platform: Device Fabrication, On-chip Assay Preparation, and System Operation

干膜 Photoresist-based 电化学微流控生物传感器平台: 器件制备、片上检测制备及系统运行

In recent years, biomarker diagnostics became an indispensable tool for the diagnosis of human disease, especially for the point-of-care diagnostics. An ...

Fabrication and Validation of an Organ-on-chip System with Integrated Electrodes to Directly Quantify Transendothelial Electrical Resistance

用集成电极直接量化 Transendothelial 电阻的 Organ-on-chip 系统的制作与验证

Organs-on-chips, in vitro models involving the culture of (human) tissues inside microfluidic devices, are rapidly emerging and promise to provide useful ...

A Lab-On-A-Chip Platform for Stimulating Osteocyte Mechanotransduction and Analyzing Functional Outcomes of Bone Remodeling

用于刺激骨质复变和分析骨骼重塑功能结果的实验室A芯片平台

Bone remodeling is a tightly regulated process that is required for skeletal growth and repair as well as adapting to changes in the mechanical ...