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DOI :
10.3791/3183-v
•
12:06 min
October 10th, 2011
Chapters
0:05
Title
1:45
Parenchymal and Non-parenchymal Separation from Whole Liver
4:34
Red Cell Lysis
5:51
CD45 Hematopoietic Cell Depletion from Non-parenchymal Fraction
7:31
Flow Cytometry Isolation of CD133 Positive Cells
9:03
Representative Cell Sorting and Analysis
11:14
Conclusion
在这里,我们描述CD133的表达肝干细胞和整体的小鼠肝,一个过程,需要组织消化,细胞富集和流式细胞仪分离肿瘤干细胞的分离。包括先进的单细胞的分离和克隆扩增的方法。
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