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DOI :
10.3791/3187-v
•
8:37 min
November 18th, 2011
Chapters
0:05
Title
1:27
Transfection of HUVEC with GFP-Actin
3:25
Set up: Live-cell Imaging Chamber and Stage Heater
5:20
Live-cell Imaging Microscope Data Acquisition and Analysis
6:14
Kymograph Analysis of HUVEC
8:08
Conclusion
GFP -アクチンを発現している生内皮細胞の顕微鏡イメージングは、細胞骨格構造の動的変化の特性評価が可能になります。固定標本を使用する技法とは異なり、この方法は前と同じ細胞内のアクチン細胞骨格の時間的変化の詳細な評価、中、および様々な物理的、薬理学的、または炎症性刺激の後にしています。
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