1. Instrumentation 
<ol>
<li>To measure the response of olfactory sensory neurons to photolysis of caged compounds we use a flash lamp in combination with a typical patch-clamp recording system including: a patch-clamp amplifier, a recording electrode and a reference electrode connected to the head-stage of a patch-clamp amplifier, a digitizer, a computer, software for data acquisition, micro-manipulators, an epifluorescence microscope, a perfusion system, an anti-vibration table and a Faraday cage (Figure 2).</li>
<li...

<ol>
	<li>Ellis-Davies, G. C. R. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Caged+compounds:+photorelease+technology+for+control+of+cellular+chemistry+and+physiology.">Caged compounds: photorelease technology for control of cellular chemistry and physiology.</a> Nat. Methods. 4, 619-628 (2007).</li><li>Pifferi, S., Boccaccio, A., Menini, A. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Cyclic+nucleotide-gated+ion+channels+in+sensory+transduction.">Cyclic nucleotide-gated ion channels in sensory transduction.</a> FEBS Lett. 580, 2853-2859 (2006).</li><li>Bozza, T. C., Kauer, J. 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L. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Flash+photolysis+of+caged+compounds.">Flash photolysis of caged compounds.</a> Microelectrodes: Theory and Applications. , (1991).</li><li>Lagostena, L., Menini, A. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Whole-cell+recordings+and+photolysis+of+caged+compounds+in+olfactory+sensory+neurons+isolated+from+the+mouse.">Whole-cell recordings and photolysis of caged compounds in olfactory sensory neurons isolated from the mouse.</a> Chem. Senses. 28, 705-716 (2003).</li><li>Boccaccio, A., Lagostena, L., Hagen, V., Menini, A. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Fast+adaptation+in+mouse+olfactory+sensory+neurons+does+not+require+the+activity+of+phosphodiesterase.">Fast adaptation in mouse olfactory sensory neurons does not require the activity of phosphodiesterase.</a> J. Gen. Physiol. 128, 171-184 (2006).</li><li>Boccaccio, A., Menini, A. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Temporal+development+of+cyclic+nucleotide-gated+and+Ca2++-activated+Cl-+currents+in+isolated+mouse+olfactory+sensory+neurons.">Temporal development of cyclic nucleotide-gated and Ca2+ -activated Cl- currents in isolated mouse olfactory sensory neurons.</a> J. 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Patch-klemp kayıtları ile birlikte kafesli bileşiklerin Flash fotoliz konsantrasyonu fizyolojik olarak aktif moleküllerin hem iç ve dış hücreleri hızlı ve yerel atlar elde etmek için çok kullanışlı bir tekniktir. Kafesli compounds1 çeşitli türlerde sentezlenen olmuştur ve bu tekniğin, kültür hücreleri aktive olabilir veya bazı kafesli bileşikler 11 fotoliz tarafından modüle iyon kanalları ifade de dahil olmak üzere hücreleri, çeşitli türleri için uygulanabilir. <p class="...

<table border="1"><tbody><tr><th> Ekipman </th><th> Şirket </th><th> Katalog numarası </th><th> Yorumlar </th></tr><tr><td> Mikroskop için adaptör modülü flaş lambası </td><td> Rapp optoelektronik </td><td> FlashCube 70 </td><td></td></tr><tr><td> Hava tablo </td><td> TMC </td><td> MİKRO-g 63-534 </td><td></td></tr><tr><td> Sayısallaştırıcı </td><td> Akson Aletleri </td><td> Digidata 1322A </td><td></td></tr><tr><td> Veri Toplama Yazılımı </td><td> Akson Aletleri </td><td> pClamp 8 </td><td></td></tr><tr><td> Veri Analizi Yazılımı </td><td> WaveMetrics </td><td> Igor </td><td></td></tr><tr><td> Ayna için adaptör modülü </td><td> Rapp optoelektronik </td><td> M70/100 </td><td></td></tr><tr><td> Elektrod tutucu </td><td> Akson Aletleri </td><td> 1-HL-U </td><td></td></tr><tr><td> Faraday kafesi </td><td> Özel yapılmış </td><td></td><td></td></tr><tr><td> Filtre küp </td><td> Olympus </td><td> U-MWU </td><td> Tahrik olma filtre kaldırıldı </td></tr><tr><td> Flaş lamba </td><td> Rapp optoelektronik </td><td> JML-C2 </td><td></td></tr><tr><td> Forseps Dumont # 55 </td><td> Dünya Hassas Aletler </td><td> 14099 </td><td></td></tr><tr><td> Cam kılcal damarlar </td><td> Dünya Hassas Aletler </td><td> PG10165-4 </td><td></td></tr><tr><td> Cam alt çanak </td><td> Dünya Hassas Aletler </td><td> FD35-100 </td><td></td></tr><tr><td> Aydınlatıcı </td><td> Olympus </td><td> Highlight 3100 </td><td></td></tr><tr><td> Ters mikroskop </td><td> Olympus </td><td> IX70 </td><td></td></tr><tr><td> Mikromanipülatörler </td><td> Luigs &amp; Neumann </td><td> SM I </td><td></td></tr><tr><td> Mikropipet Çektirme </td><td> Narishige </td><td> PP-830 </td><td></td></tr><tr><td> Izlemek </td><td> HesaVision </td><td> MTB-01 </td><td></td></tr><tr><td> Nötr yoğunluk filtreleri </td><td> Omega Optik </td><td> değişir </td><td></td></tr><tr><td> Amaç 100X </td><td> Zeiss </td><td> Fluar 440.285 </td><td> Ya Zeiss veya Olympus </td></tr><tr><td> Amaç 100X </td><td> Olympus </td><td> UPLFLN 100XOI2 </td><td> Ya Zeiss veya Olympus </td></tr><tr><td> Optik UV shortpass filtresi </td><td> Rapp optoelektronik </td><td> SP400 </td><td></td></tr><tr><td> Patch-kelepçe amplifikatör </td><td> Akson Aletleri </td><td> Axopatch 200B </td><td></td></tr><tr><td> Foto Diyot Meclisi </td><td> Rapp optoelektronik </td><td> PDA </td><td></td></tr><tr><td> Kuvars ışık kılavuzu </td><td> Rapp optoelektronik </td><td> değişir </td><td> Biz 600 mikron çapında </td></tr><tr><td> Gümüş tel </td><td> Dünya Hassas Aletler </td><td> AGT1025 </td><td></td></tr><tr><td> Gümüş toprak pelet </td><td> Warner aletleri </td><td> 64-1309 </td><td></td></tr><tr><td> Xenon ark lambası </td><td> Rapp optoelektronik </td><td> XBL-JML </td><td></td></tr></tbody></table> <table border="1"><tbody><tr><th> Reaktif </th><th> Şirket </th><th> Katalog numarası </th></tr><tr><td> BCMCM-kafesli cAMP </td><td> Biolog </td><td> B016 </td></tr><tr><td> Sığır serum albumini (BSA) </td><td> Sigma </td><td> A8806 </td></tr><tr><td> CaCl2 standard çözeltisi 0,1 M </td><td> Fluka </td><td> 21059 </td></tr><tr><td> Kafesli Ca: DMNP-EDTA </td><td> Invitrogen </td><td> D6814 </td></tr><tr><td> Sistein </td><td> Sigma </td><td> C9768 </td></tr><tr><td> Concanavalin A tipi V (CONA) </td><td> Sigma </td><td> C7275 </td></tr><tr><td> CSCL </td><td> Sigma </td><td> C4036 </td></tr><tr><td> DMSO </td><td> Sigma </td><td> D8418 </td></tr><tr><td> DNAz I </td><td> Sigma </td><td> D4527 </td></tr><tr><td> EDTA </td><td> Sigma </td><td> E9884 </td></tr><tr><td> EGTA </td><td> Sigma </td><td> E4378 </td></tr><tr><td> Glikoz </td><td> Sigma </td><td> G5767 </td></tr><tr><td> Hepes </td><td> Sigma </td><td> H3375 </td></tr><tr><td> KCl </td><td> Sigma </td><td> P3911 </td></tr><tr><td> KOH </td><td> Sigma </td><td> P1767 </td></tr><tr><td> Leupeptin </td><td> Sigma </td><td> L0649 </td></tr><tr><td> MgCl2 </td><td> Fluka </td><td> 63020 </td></tr><tr><td> Papain </td><td> Sigma </td><td> P3125 </td></tr><tr><td> Poli-L-lisin </td><td> Sigma </td><td> P1274 </td></tr><tr><td> NaCl </td><td> Sigma </td><td> S9888 </td></tr><tr><td> NaOH </td><td> Sigma </td><td> S5881 </td></tr><tr><td> NaPyruvate </td><td> Sigma </td><td> P2256 </td></tr></tbody></table>

flash photolysis of caged compounds in the cilia of olfactory sensory  neurons

Kafesli bileşiklerin Fotoliz hızlı ve yerelleştirilmiş çeşitli fizyolojik olarak aktif bileşikler 1 konsantrasyonu artar üretimi sağlar. Kafesli bileşikler, ultraviyole ışığın bir flash kırılmış olabilir kimyasal bir kafes tarafından fizyolojik olarak inaktif hale moleküllerdir. Burada, ayrışmış fare koku alma duyu nöronlar koku iletimi çalışması için kafesli bileşiklerin fotoliz ile birlikte patch-klemp kayıtları nasıl elde göstermektedir. Reseptörlerine bağlanarak odorant siklik nükleotid kapılı (CNG) kanal 2 açılır cAMP artışına yol açan koku alma duyu nöronlar, kirpikler, koku alma transdüksiyon işlemi (Şekil 1) yer alır . Ca CNG kanallar aracılığıyla giriş aktive Ca-Cl kanalları harekete geçirir. Biz, fare olfaktör epitel 3 ve kafesli cAMP 4 fotoliz veya Ca 5 kafesli CNG kanalları ya da Ca-aktive Cl kanalları nasıl aktif hale getirmek için nöronların nasıl ayırmak &lt;/&gt; Destek. Biz tam hücreli gerilim kelepçe yapılandırma 8-11 patch-klemp kayıtları mevcut ölçmek için alınır uncage cAMP veya Ca ultraviyole yanıp söner siliyer bölgeye uygulamak için bir flash lamba 6,7 kullanın.

Watch this Scientific Journal Video about Flash Photolysis of Caged Compounds in the Cilia of Olfactory Sensory  Neurons at JoVE.com

Flash Photolysis of Caged Compounds in the Cilia of Olfactory Sensory  Neurons

Kafesli bileşiklerin Fotoliz çeşitli fizyolojik aktif bileşiklerin konsantrasyonu hızlı ve yerelleştirilmiş artar üretimi sağlar. Burada, kafesli cAMP fotoliz ile kombine veya ayrışmış bir fare koku alma duyusal nöronlarda koku iletimi çalışma için Ca kafesli patch-klemp kayıtları nasıl elde göstermektedir.

Koku Duyu Nöronlar Cilia Kafesli Bileşiklerin Flash Fotoliz

Photolysis of caged compounds allows the production of rapid and localized increases in the concentration of various physiologically active compounds1. ...

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In Koku Reseptör Nöronlar Tüm Dağı ile immün<em&gt; Drosophila</em&gt; Anten

Odorant molecules bind to their target receptors in a precise and coordinated manner. Each receptor recognizes a specific signal and relays this ...

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Fareler Ana Koku Sistemde Cilia Whole Dağı Etiketleme

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