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57.3K Views
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08:01 min
December 22nd, 2011
DOI :
10.3791/3227-v
Chapters
0:05
Title
1:16
Making Carbon Coated EM Grids for Negative Staining EM
2:28
Carbon Coating Grids
4:27
Preparing Negative Stain EM Grids
5:57
Preparing an Electron Microscope
7:07
Electron Microscopy Images
7:30
Conclusion
Summary
负染色电子显微镜(EM)的可视化蛋白质样品已成为一种流行的结构分析方法。它是有用的定量结构分析,如计算一个正在研究的分子的三维重建,也为蛋白质制剂质量的定性检查。在这篇文章中,我们目前准备的EM电网,染色样品,并在电子显微镜下的可视化样品的详细协议。新手用户可以轻松地遵循这些协议,并利用负染作为常规检测EM,除了其他生化分析评估其蛋白质样品,。
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