Name: development of a unilaterally-lesioned 6-ohda mouse model of parkinson's disease
Uploaded: 2012-02-14T12:00:00
Description: Watch this Scientific Journal Video about Development of a Unilaterally-lesioned 6-OHDA Mouse Model of Parkinson's Disease at JoVE.com

这项工作是支持的外交事务和国际贸易（加拿大政府），多伦多大学，加拿大创新，NSERC，Krembil的基金会和治疗帕金森氏信托基金会诺基金。

1。住房和小鼠的制备<ol><li>这些老鼠是纯FVB小鼠，保持驱动的转基因小鼠8（突变小鼠区域资源中心（MMRRC）的FVB在12:12 h光照黑暗免费获得食物和水的循环细菌人工染色体（BAC）的殖民地。越过这些小鼠与任何其他应变是没有必要的，无论是繁殖的目的，或6-OHDA损伤过程，以确保成功。 </li><li>为了产生帕金森病模型，出生后一天31-42岁的成年小鼠（P31-42）6-OHDA损伤和假手术的需要。 </li><...

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本协议描述了一个稳定的单方面6-OHDA损毁的帕金森氏症，这是非常重复性高病变的成功率，低死亡率，小鼠模型的生成方法。 6-OHDA损伤手术的成功，可以很容易地估计&gt; 70％&gt; 95％，在27的损毁纹状体多巴胺耗竭的指标ipsiversive旋转自发旋转行为的测量。纹状体多巴胺水平的量化纹状体多巴胺耗竭的程度相比，在黑质致密部TH阳性细胞数量quantificiation是最精确的测量，因为它提供了直接?...

<table border="1" &gt;<tbody&gt;<tr &gt;<td<strong&gt;试剂名称</strong</td&gt;<td<strong&gt;公司</strong</td&gt;<td<strong&gt;产品目录号</strong</td&gt;<td<strong&gt;评论（可选）</strong</td&gt;</tr&gt;<tr &gt;<td&gt; desipramine的盐酸</td&gt;<td&gt;Σ-Aldrich公司，奥克维尔，ON，加拿大</td&gt;<td&gt; D125</td&gt;<td&gt; 25毫克</td&gt;</tr&gt;<tr&gt;<td&gt;优降宁盐酸</td&gt;<td&gt;Σ-Aldrich公司，奥克维尔，ON，加拿大</td&gt;<td&gt; P8013</td&gt;<td&gt;按5mg/kg</td&gt;</tr&gt;<tr&gt;<td&gt; 6 - 羟基多巴胺氢溴酸</td&gt;<td&gt;Σ-Aldrich公司，奥克维尔，ON，加拿大</td&gt;<td&gt; H116</td&gt;<td&gt; 3毫克/鼠标</td&gt;</tr&gt;<tr&gt;<td&gt;立体框架</td&gt;<td&gt; Kopf仪器，图洪加，CA，美国</td&gt;<td&gt;型号900</td&gt;<td&gt;</td&gt;</tr&gt;<tr&gt;<td&gt;小鼠耳杯</td&gt;<td&gt; Kopf仪器，图洪加，CA，美国</td&gt;<td&gt;型号921颧骨耳罩</td&gt;<td&gt;</td&gt;</tr&gt;<tr&gt;<td&gt;鼠标切牙酒吧</td&gt;<td&gt; Kopf仪器，图洪加，CA，美国</td&gt;<td&gt;型号923B</td&gt;<td&gt;</td&gt;</tr&gt;<tr&gt;<td&gt;鼠标麻醉面罩</td&gt;<td&gt; Kopf仪器，图洪加，CA，美国</td&gt;<td&gt;型号923B</td&gt;<td&gt;</td&gt;</tr&gt;<tr&gt;<td&gt;吸套件（含250毫升注射器）</td&gt;<td&gt;汉密尔顿公司，里诺，内华达州，美国</td&gt;<td&gt; PRMKIT 81120</td&gt;<td&gt;</td&gt;</tr&gt;<tr&gt;<td&gt; RN压缩接头套件（1毫米）</td&gt;<td&gt;汉密尔顿公司，里诺，内华达州，美国</td&gt;<td&gt; 55750-01</td&gt;<td&gt;</td&gt;</tr&gt;<tr&gt;<td&gt; PEEK管RN压缩接头套件<
 (1/16<sup&gt;日</sup&gt;英寸）</td&gt;<td&gt;汉密尔顿公司，里诺，内华达州，美国</td&gt;<td&gt; 55751-01</td&gt;<td&gt;</td&gt;</tr&gt;<tr&gt;<td小双枢纽护士耦合器</td&gt;<td&gt;汉密尔顿公司，里诺，内华达州，美国</td&gt;<td&gt; 55752-01</td&gt;<td&gt;</td&gt;</tr&gt;<tr&gt;<td&gt;露儿到小枢纽护士适配器</td&gt;<td&gt;汉密尔顿公司，里诺，内华达州，美国</td&gt;<td&gt; 55753-01</td&gt;<td&gt;</td&gt;</tr&gt;<tr&gt;<td&gt;1毫升25S注射器模型7001KH</td&gt;<td&gt;汉密尔顿公司，里诺，内华达州，美国</td&gt;<td&gt; 80100</td&gt;<td&gt;</td&gt;</tr&gt;<tr&gt;<td&gt; * 33G可拆卸的针头（RN）的包6。 。自定义1英寸45 &lt;°斜角</td&gt;<td&gt;汉密尔顿公司，里诺，内华达州，美国</td&gt;<td&gt; 7803-05</td&gt;<td&gt;</td&gt;</tr&gt;<tr&gt;<td&gt;剪刀</td&gt;<td&gt;精细的科学工具，温哥华，不列颠哥伦比亚省，加拿大。</td&gt;<td&gt; 14084-08</td&gt;<td&gt;</td&gt;</tr&gt;<tr&gt;<td&gt;手术刀</td&gt;<td&gt;精细的科学工具，温哥华，不列颠哥伦比亚省，加拿大</td&gt;<td&gt; 10003-12</td&gt;<td&gt;</td&gt;</tr&gt;<tr&gt;<td&gt;手术刀片</td&gt;<td&gt;精细的科学工具，温哥华，不列颠哥伦比亚省，加拿大</td&gt;<td&gt; 10035-20</td&gt;<td&gt;</td&gt;</tr&gt;<tr&gt;<td&gt;夹钳</td&gt;<td&gt;精细的科学工具，温哥华，不列颠哥伦比亚省，加拿大</td&gt;<td&gt; 11608-15</td&gt;<td&gt;</td&gt;</tr&gt;<tr&gt;<td&gt;止血</td&gt;<td&gt;精细的科学工具，温哥华，不列颠哥伦比亚省，加拿大。</td&gt;<td&gt; 13004-14</td&gt;<td&gt;</td&gt;</tr&gt;<tr&gt;<td&gt;异氟醚</td&gt;<td&gt;住持</td&gt;<td&gt; 02241315</td&gt;<td&gt; 2-3％</td&gt;</tr&gt;<tr&gt;<td&gt; Suters（Vicryl 4.0）</td&gt;<td&gt; Syneture</td&gt;<td&gt; SS-683</td&gt;<td&gt;</td&gt;</tr&gt;<tr&gt;<td&gt;消毒器</td&gt;<td&gt;精细的科学工具，温哥华，不列颠哥伦比亚省，加拿大</td&gt;<td&gt; 18000-45</td&gt;<td&gt;</td&gt;</tr&gt;<tr&gt;<td&gt;输液泵</td&gt;<td&gt;哈佛仪器</td&gt;<td&gt;博士22/2000</td&gt;<td&gt;</td&gt;</tr&gt;<tr&gt;<td&gt;针（27G）</td&gt;<td&gt;碧迪</td&gt;<td&gt; 305109</td&gt;<td&gt;</td&gt;</tr&gt;<tr&gt;<td&gt;针（25G）</td&gt;<td&gt;碧迪</td&gt;<td&gt; 305127</td&gt;<td&gt;</td&gt;</tr&gt;<tr&gt;<td注射器（1毫升）</td&gt;<td&gt;屋宇署注射器</td&gt;<td&gt; 309692</td&gt;<td&gt;</td&gt;</tr&gt;<tr&gt;<td&gt;麻醉手推车</td&gt;<td&gt;雷医疗</td&gt;<td&gt; M2000型</td&gt;<td&gt;</td&gt;</tr&gt;<tr&gt;<td&gt; Baytril</td&gt;<td&gt; CDMV，圣圣亚森特，QC</td&gt;<td&gt; 102207</td&gt;<td&gt;</td&gt;</tr&gt;<tr&gt;<td&gt;利多卡因</td&gt;<td&gt; CDMV，圣圣亚森特，QC</td&gt;<td&gt; 3914</td&gt;<td&gt;</td&gt;</tr&gt;<tr&gt;<td&gt;优碘溶液</td&gt;<td&gt; CDMV，圣圣亚森特，QC</td&gt;<td&gt; 19955</td&gt;<td&gt;</td&gt;</tr&gt;</tbody&gt;</table

development of a unilaterally-lesioned 6-ohda mouse model of parkinson's disease

单方面损毁6 hyroxydopamine（6 - 羟基多巴胺），损毁帕金森病大鼠模型（PD）的已证实到是在推进我们基本巴金森氏症症状的机制的理解非常宝贵的，因为它概括在基底神经节电路的变化和药理学观察中帕金森患者1-4。然而，在纹状体内的输出途径，这是基底节的主要输入地区的皮质纹状体突触发生的确切的细胞和分子的变化仍然是难以捉摸的，这被认为是网站，在这里出现潜在的帕金森病症状的病理异常5。 在PD，了解基底节电路以下尼格罗 - 纹状体通路变性的变化机制已大大提前过度表达绿色荧光蛋白，通过促进推动发展由细菌人工染色体（BAC）的小鼠RS具体纹状体两个输出途径（EGFP-D1的直接途径，间接的途径：EGFP-D2和EGFP-A2A）让他们在隔离研究。例如，最近的研究表明，有在突触可塑性的病理变化在帕金森病小鼠9,10。然而，这些研究利用幼年小鼠和急性帕金森模型。目前还不清楚是否与稳定的6 - 羟多巴胺损毁大鼠中所描述的变化也发生在这些模型中。其他团体都试图产生一个稳定的单方面的损毁6-OHDA成年PD小鼠模型，损毁内侧前脑束（MFB），不幸的是，在这项研究中的死亡率是非常高，只有14％存活21手术天或更长时间11。最近的研究已产生内黑质病变与低死亡率&gt; 80％的多巴胺能神经元的损失，但表达左旋多巴引起的运动障碍11,12,13,14变量在这些研究中。另一个PD小鼠模型MPTP的损伤小鼠15。虽然这种模式已被证明在评估潜在神经保护剂16有用的，它是不适合理解机制帕金森病的症状，这种模式往往未能引起运动障碍，在18 17病变程度，显示了一个很大的可变性。 在这里，我们已经开发了6-OHDA直接管理到稳定单方面的PD小鼠模型6-OHDA损毁MFB的，一贯导致95％的损失纹状体多巴胺（高效液相色谱法测定），以及生产的行为，不平衡的特点以及单方面的6-OHDA损毁PD大鼠模型中观察到。这个新开发的PD小鼠模型，将被证明是有价值的工具，在了解帕金森病症状的生成机制。

Watch this Scientific Journal Video about Development of a Unilaterally-lesioned 6-OHDA Mouse Model of Parkinson's Disease at JoVE.com

Development of a Unilaterally-lesioned 6-OHDA Mouse Model of Parkinson's Disease

一个6-OHDA单侧病变，内侧前脑束对小鼠进行的协议说明。该方法具有低死亡率与89％的幸存的动物&gt; 95％的损失纹状体多巴胺和90.63率（13.3％）±-4.02％ipsiversive旋转对病灶侧的偏见。

1单侧损毁6  - 羟基多巴胺帕金森病小鼠模型的发展

The unilaterally lesioned 6-hyroxydopamine (6-OHDA)-lesioned rat model of Parkinson's disease (PD) has proved to be invaluable in advancing our ...

Research

JoVE Journal

Medicine

MALDI Imaging Mass Spectrometry of Neuropeptides in Parkinson's Disease

MALDI成像帕金森病的神经肽质谱

MALDI imaging mass spectrometry (IMS) is a powerful approach that facilitates the spatial analysis of molecular species in biological tissue samples2 ...

科研

医学

Skin Punch Biopsy Explant Culture for Derivation of Primary Human Fibroblasts

推导人类纤维原细胞的皮肤穿刺活检植文化

Tissues and cell lines derived from an individual with disease are ideal sources to study disease-related cellular phenotypes. Patient-derived fibroblasts ...

The Use of Primary Human Fibroblasts for Monitoring Mitochondrial Phenotypes in the Field of Parkinson's Disease

利用人类纤维原细胞线粒体表型的监测领域的帕金森氏病

Parkinson's disease (PD) is the second most  common movement disorder and affects 1% of people over the age of 60 1. Because ageing is  the most important ...

A Simple Method of Mouse Lung Intubation

一个简单的方法小鼠肺插管

A  simple procedure to intubate mice for pulmonary function measurements would  have several advantages in longitudinal studies with limited numbers or  ...

Gene-environment Interaction Models to Unmask Susceptibility Mechanisms in Parkinson's Disease

基因-环境相互作用模型揭开帕金森病的隐形易感机制

Lipoxygenase (LOX) activity has been implicated in neurodegenerative disorders such as Alzheimer&#39;s disease, but its effects in Parkinson&#39;s disease ...

Controlling Parkinson's Disease With Adaptive Deep Brain Stimulation

控制帕金森氏症与自适应深部脑刺激

Adaptive deep brain stimulation (aDBS) has the potential to improve the treatment of Parkinson&#39;s disease by optimizing stimulation in real time ...

Establishment and Characterization of UTI and CAUTI in a Mouse Model

建立和UTI和CAUTI表征在小鼠模型

Urinary tract infections (UTI) are highly prevalent, a significant cause of morbidity and are increasingly resistant to treatment with antibiotics. ...

A Quick Phenotypic Neurological Scoring System for Evaluating Disease Progression in the SOD1-G93A Mouse Model of ALS

一个快速的表型神经评分系统在ALS的SOD1-G93A小鼠模型评估疾病进展

The SOD1-G93A transgenic mouse is the most widely used animal model of amyotrophic lateral sclerosis (ALS). At ALS TDI we developed a phenotypic screening ...

A Neonatal Mouse Spinal Cord Compression Injury Model

新生儿小鼠脊髓压迫损伤模型

Spinal cord injury (SCI) typically causes devastating neurological deficits, particularly through damage to fibers descending from the brain to the spinal ...

Development of an Alpha-synuclein Based Rat Model for Parkinson's Disease via Stereotactic Injection of a Recombinant Adeno-associated Viral Vector

通过重组腺相关病毒载体注射立体的α-突触核蛋白基于大鼠模型为帕金森氏病的发展

In order to study the molecular pathways of Parkinson&#39;s disease (PD) and to develop novel therapeutic strategies, scientific investigators rely on ...