Name: development of a unilaterally-lesioned 6-ohda mouse model of parkinson's disease
Uploaded: 2012-02-14T12:00:00
Description: Watch this Scientific Journal Video about Development of a Unilaterally-lesioned 6-OHDA Mouse Model of Parkinson's Disease at JoVE.com

Bu çalışma, Dışişleri Bakanlığı ve Uluslararası Ticaret (Kanada Hükümeti), University of Toronto Connaught Fonu, Yenilik, NSERC, Krembil Vakfı ve için Cure Parkinson Güven Kanada Vakfı tarafından desteklenmiştir.

1. Konut ve fareler hazırlanması <ol><li> Transgenik fareler tahrik 8 (gıda ve suya ücretsiz erişim ile 12:12 saat aydınlık-karanlık döngüsü Mutant Fare Bölgesel Kaynak Merkezi (MMRRC) FVB bakteriyel yapay kromozom (BAC) bir koloni koruyun. Bu fareler, saf FVB fare ve amaçlı ıslah için, ya başka herhangi bir zorlanma ile bu farelerin geçmek için gerekli değildir, ya da 6-OHDA lezyon prosedürün başarı sağlamak için. </li><li> Parkinson bir model oluşturmak için, doğum sonrası g?...

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Bu protokol bir lezyon başarı oranı yüksek ve düşük mortalite oranı, son derece tekrarlanabilir Parkinson hastalığı, istikrarlı bir tek taraflı 6-OHDA-lezyonlanan fare modelinin üretimi için bir yöntem açıklanır. , 6-OHDA lezyon cerrahisi başarı kolayca lezyonlu striatum 27&gt;% 95 dopamin eksikliği göstergesidir&gt;% 70 ipsiversive rotasyonlar ile ölçümü ile spontan dönme davranışı tahmin edilebilir. Striatal dopamin düzeylerini 28 doğrudan ölçüm sağlar striata...

<table border="1" &gt;<tbody&gt;<tr &gt;<td<strong&gt; Ad reaktif</strong</td&gt;<td<strong&gt; Şirket</strong</td&gt;<td<strong&gt; Katalog numarası</strong</td&gt;<td<strong&gt; Yorumlar (isteğe bağlı)</strong</td&gt;</tr&gt;<tr &gt;<td&gt; Desipramin HCl</td&gt;<td&gt; Sigma-Aldrich, Oakville, ON, Kanada</td&gt;<td&gt; D125</td&gt;<td&gt; 25mg/kg</td&gt;</tr&gt;<tr&gt;<td&gt; ILM parglin HCl</td&gt;<td&gt; Sigma-Aldrich, Oakville, ON, Kanada</td&gt;<td&gt; P8013</td&gt;<td&gt; 5mg/kg</td&gt;</tr&gt;<tr&gt;<td&gt; 6-OHDA HBr</td&gt;<td&gt; Sigma-Aldrich, Oakville, ON, Kanada</td&gt;<td&gt; H116</td&gt;<td&gt; 3mg / fare</td&gt;</tr&gt;<tr&gt;<td&gt; Stereotaksik Çerçeve</td&gt;<td&gt; Kopf Instruments Tujunga ile, CA, USA</td&gt;<td&gt; Model 900</td&gt;<td&gt;</td&gt;</tr&gt;<tr&gt;<td&gt; Fare kulak kepçesi</td&gt;<td&gt; Kopf Instruments Tujunga ile, CA, USA</td&gt;<td&gt; Model 921 elmacık kemiği Kulak Bardaklar</td&gt;<td&gt;</td&gt;</tr&gt;<tr&gt;<td&gt; Fare kesici bar</td&gt;<td&gt; Kopf Instruments Tujunga ile, CA, USA</td&gt;<td&gt; Model 923B</td&gt;<td&gt;</td&gt;</tr&gt;<tr&gt;<td&gt; Fare anestezi maskesi</td&gt;<td&gt; Kopf Instruments Tujunga ile, CA, USA</td&gt;<td&gt; Model 923B</td&gt;<td&gt;</td&gt;</tr&gt;<tr&gt;<td&gt; Priming kiti (250ml şırınga içeren)</td&gt;<td&gt; Hamilton Şirket, Reno, NV, ABD</td&gt;<td&gt; PRMKIT 81.120</td&gt;<td&gt;</td&gt;</tr&gt;<tr&gt;<td&gt; RN sıkıştırma montaj kiti (1 mm)</td&gt;<td&gt; Hamilton Şirket, Reno, NV, ABD</td&gt;<td&gt; 55.750-01</td&gt;<td&gt;</td&gt;</tr&gt;<tr&gt;<td&gt; RN sıkıştırma montaj kiti PEEK boru<
 (1/16<sup&gt; Th</sup&gt; Inç)</td&gt;<td&gt; Hamilton Şirket, Reno, NV, ABD</td&gt;<td&gt; 55.751-01</td&gt;<td&gt;</td&gt;</tr&gt;<tr&gt;<td&gt; Çift küçük hub RN çoğaltıcı</td&gt;<td&gt; Hamilton Şirket, Reno, NV, ABD</td&gt;<td&gt; 55.752-01</td&gt;<td&gt;</td&gt;</tr&gt;<tr&gt;<td&gt; Luer küçük hub RN adaptörü</td&gt;<td&gt; Hamilton Şirket, Reno, NV, ABD</td&gt;<td&gt; 55.753-01</td&gt;<td&gt;</td&gt;</tr&gt;<tr&gt;<td&gt; 1ml 25S şırınga modeli 7001KH</td&gt;<td&gt; Hamilton Şirket, Reno, NV, ABD</td&gt;<td&gt; 80100</td&gt;<td&gt;</td&gt;</tr&gt;<tr&gt;<td&gt; * 6 33G çıkarılabilir iğne (RN) paket. . 45 &lt;° eğim ile Özel 1 inç</td&gt;<td&gt; Hamilton Şirket, Reno, NV, ABD</td&gt;<td&gt; 7803-05</td&gt;<td&gt;</td&gt;</tr&gt;<tr&gt;<td&gt; Makas</td&gt;<td&gt; Güzel Bilim Araçlar, Vancouver, BC, Kanada.</td&gt;<td&gt; 14.084-08</td&gt;<td&gt;</td&gt;</tr&gt;<tr&gt;<td&gt; Neşter</td&gt;<td&gt; Güzel Bilim Araçlar, Vancouver, BC, Kanada</td&gt;<td&gt; 10.003-12</td&gt;<td&gt;</td&gt;</tr&gt;<tr&gt;<td&gt; Neşter bıçakları</td&gt;<td&gt; Güzel Bilim Araçlar, Vancouver, BC, Kanada</td&gt;<td&gt; 10.035-20</td&gt;<td&gt;</td&gt;</tr&gt;<tr&gt;<td&gt; Forsepsle</td&gt;<td&gt; Güzel Bilim Araçlar, Vancouver, BC, Kanada</td&gt;<td&gt; 11.608-15</td&gt;<td&gt;</td&gt;</tr&gt;<tr&gt;<td&gt; Hemostat</td&gt;<td&gt; Güzel Bilim Araçlar, Vancouver, BC, Kanada.</td&gt;<td&gt; 13.004-14</td&gt;<td&gt;</td&gt;</tr&gt;<tr&gt;<td&gt; İsofluranın</td&gt;<td&gt; Abbot</td&gt;<td&gt; 02241315</td&gt;<td&gt;% 2-3</td&gt;</tr&gt;<tr&gt;<td&gt; Suters (Vicryl 4.0)</td&gt;<td&gt; Syneture</td&gt;<td&gt; SS-683</td&gt;<td&gt;</td&gt;</tr&gt;<tr&gt;<td&gt; Sterilizatör</td&gt;<td&gt; Güzel Bilim Araçlar, Vancouver, BC, Kanada</td&gt;<td&gt; 18.000-45</td&gt;<td&gt;</td&gt;</tr&gt;<tr&gt;<td&gt; İnfüzyon Pompası</td&gt;<td&gt; Harvard Apparatus</td&gt;<td&gt; Doktora 22/2000.</td&gt;<td&gt;</td&gt;</tr&gt;<tr&gt;<td&gt; İğneler (27G)</td&gt;<td&gt; Becton Dickinson</td&gt;<td&gt; 305.109</td&gt;<td&gt;</td&gt;</tr&gt;<tr&gt;<td&gt; İğneler (25G)</td&gt;<td&gt; Becton Dickinson</td&gt;<td&gt; 305.127</td&gt;<td&gt;</td&gt;</tr&gt;<tr&gt;<td&gt; Enjektörler (1 ml)</td&gt;<td&gt; BD şırınga</td&gt;<td&gt; 309.692</td&gt;<td&gt;</td&gt;</tr&gt;<tr&gt;<td&gt; Anestezi arabası</td&gt;<td&gt; LEI tıbbi</td&gt;<td&gt; M2000</td&gt;<td&gt;</td&gt;</tr&gt;<tr&gt;<td&gt; Baytril</td&gt;<td&gt; CDMV, St. Hyacinthe&#39;dan, QC</td&gt;<td&gt; 102.207</td&gt;<td&gt;</td&gt;</tr&gt;<tr&gt;<td&gt; Lidokain</td&gt;<td&gt; CDMV, St. Hyacinthe&#39;dan, QC</td&gt;<td&gt; 3914</td&gt;<td&gt;</td&gt;</tr&gt;<tr&gt;<td&gt; Betadine çözüm</td&gt;<td&gt; CDMV, St. Hyacinthe&#39;dan, QC</td&gt;<td&gt; 19.955</td&gt;<td&gt;</td&gt;</tr&gt;</tbody&gt;</table

development of a unilaterally-lesioned 6-ohda mouse model of parkinson's disease

Bu özetlediği bazal ganglionlar devresi değişiklikleri ve farmakoloji olarak gözlenen bu yana tek taraflı olarak lezyonlanmıştır 6 hyroxydopamine (6-OHDA)-lezyonlanan Parkinson hastalığı sıçan modeli (PD), parkinson belirtileri altında yatan mekanizmaları anlayışımıza ilerlemede paha biçilmez olmak için ispat etti Parkinsonlu hastalarda 1-4. Bununla birlikte,, çıkış yollarının, başlıca, bazal ganglia bir giriş bölgesi olan striatum, dahilinde kortiko-striatal sinapsların oluşan hassas hücresel ve moleküler değişiklik zor kalır, ve bu patolojik anormallikleri parkinson arazlarının yatan 3 ortaya çıkan sitenin olduğuna inanılmaktadır 5. PD, bazal ganglion devresi-striatal Nigro yolunun aşağıdaki dejenerasyon değişikliklerin altında yatan mekanizmaları anlamak, büyük ölçüde teşvik tarafından yönlendirilen aşırı eksprese yeşil floresan proteinleri gelişimi bakteriyel yapay kromozom (BAC) fareler tarafından ileri olmuşturrs özel izolasyon çalışılması gerekir izin vererek 8:; iki striatal çıkış yolları (EGFP-D2 ve EGFP-A2a dolaylı yolun EGFP-D1 doğrudan yolak). Örneğin, son yıllarda yapılan çalışmalarda, parkinson fareler 9,10 sinaptik plastisite patolojik değişiklikler olduğunu ileri sürmüşlerdir. Ancak, bu çalışmalar yavru fareler ve parkinsonizm akut modelleri kullandı. 6-OHDA stabil lezyonlar ile erişkin sıçanlarda açıklanan değişimler de bu modellerde oluşup oluşmadığına belirsizdir. Diğer gruplar medial ön beyin demeti (MFB) lezyon PD tek taraflı lezyonlanan istikrarlı bir 6-OHDA yetişkin fare modeli oluşturmak için çalıştılar, ne yazık ki, bu çalışmada mortalite oranı sadece 14% 21 cerrahi kalan, son derece yüksek oldu gün veya daha uzun 11. Daha yeni çalışmalarda, düşük bir mortalite oranı ile intra-nigral lezyonlar dopaminerjik nöronlar&gt;% 80 kaybı, L-DOPA bağlı diskinezi 11,12,13,14 Ancak ifade değişkenBu çalışmalar. , PD iyi kurulmuş bir başka fare modeli, MPTP-lezyonlanan fare 15. Iken, bu model potansiyel nöroprotektif ajanların 16 değerlendirme yararlı olan bu modeli genellikle motor açıklarının neden başarısız, PD belirtileri altında yatan mekanizmaları anlamak için daha uygun ve geniş bir lezyon 17, 18 ölçüde değişkenlik gösterir . Burada içine doğrudan 6-OHDA, uygulama tarafından, tutarlı&gt;% 95 striatal dopamin kaybı (gibi HPLC ile ölçülen) yanı sıra, davranışsal üreten neden MFB, stabil bir PD unilateral 6-OHDA-lezyonlu fare modelinde geliştirilmiştir. dengesizliklerin PD iyi karakterize tek taraflı 6-OHDA-lezyonlanan sıçan modelinde gözlendi. PD Bu yeni geliştirilen bir fare modelinde, parkinson arazlarının nesil altında yatan mekanizmaları anlamak için değerli bir araç ispat edecektir.

Watch this Scientific Journal Video about Development of a Unilaterally-lesioned 6-OHDA Mouse Model of Parkinson's Disease at JoVE.com

Development of a Unilaterally-lesioned 6-OHDA Mouse Model of Parkinson's Disease

Bir protokol unilateral 6-OHDA lezyonlar, farelerde medyal önbeyin demet gerçekleştirmek için tarif edilmektedir. Bu yöntem, striatal dopamin ve 90,63&gt;% 95 kaybı gösteren hayatta kalan hayvanların% 89 ile düşük mortalite oranı (% 13.3) ±% -4,02, lezyonun tarafa doğru ipsiversive dönme önyargı vardır.

Parkinson Hastalığının tek taraflı lezyonlanmıştır 6-OHDA Mouse Model Geliştirme

The unilaterally lesioned 6-hyroxydopamine (6-OHDA)-lesioned rat model of Parkinson's disease (PD) has proved to be invaluable in advancing our ...

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Medicine

MALDI Imaging Mass Spectrometry of Neuropeptides in Parkinson's Disease

Parkinson Hastalığında nöropeptitler MALDI Görüntüleme Kütle Spektrometresi

MALDI imaging mass spectrometry (IMS) is a powerful approach that facilitates the spatial analysis of molecular species in biological tissue samples2 ...

Skin Punch Biopsy Explant Culture for Derivation of Primary Human Fibroblasts

İlköğretim İnsan Fibroblastlar türetilmesi için Cilt Punch Biyopsi Eksplantın Kültür

Tissues and cell lines derived from an individual with disease are ideal sources to study disease-related cellular phenotypes. Patient-derived fibroblasts ...

The Use of Primary Human Fibroblasts for Monitoring Mitochondrial Phenotypes in the Field of Parkinson's Disease

Parkinson Hastalığı Alanında Mitokondriyal Fenotiplerinin İzleme İlköğretim İnsan Fibroblast Kullanımı

Parkinson's disease (PD) is the second most  common movement disorder and affects 1% of people over the age of 60 1. Because ageing is  the most important ...

A Simple Method of Mouse Lung Intubation

Fare Akciğer Entübasyonun Basit Bir Yöntem

A  simple procedure to intubate mice for pulmonary function measurements would  have several advantages in longitudinal studies with limited numbers or  ...

Gene-environment Interaction Models to Unmask Susceptibility Mechanisms in Parkinson's Disease

Parkinson Hastalığında Maskeyi Düşürme Duyarlılık Mekanizmalarına Gen-Çevre Etkileşim Modelleri

Lipoxygenase (LOX) activity has been implicated in neurodegenerative disorders such as Alzheimer&#39;s disease, but its effects in Parkinson&#39;s disease ...

Controlling Parkinson's Disease With Adaptive Deep Brain Stimulation

Adaptif Derin Beyin Stimülasyonu İle Parkinson Hastalığı kontrol

Adaptive deep brain stimulation (aDBS) has the potential to improve the treatment of Parkinson&#39;s disease by optimizing stimulation in real time ...

Establishment and Characterization of UTI and CAUTI in a Mouse Model

Fare Modelinde Kuruluş ve İYE ve CAUTI Karakterizasyonu

Urinary tract infections (UTI) are highly prevalent, a significant cause of morbidity and are increasingly resistant to treatment with antibiotics. ...

A Quick Phenotypic Neurological Scoring System for Evaluating Disease Progression in the SOD1-G93A Mouse Model of ALS

ALS SOD1-G93A Fare Modeli hastalığın ilerlemesine değerlendirilmesi için Hızlı Fenotipik Nörolojik Puanlama Sistemi

The SOD1-G93A transgenic mouse is the most widely used animal model of amyotrophic lateral sclerosis (ALS). At ALS TDI we developed a phenotypic screening ...

A Neonatal Mouse Spinal Cord Compression Injury Model

Bir Yenidoğan Fare Omurilik Sıkıştırma Yaralanması Modeli

Spinal cord injury (SCI) typically causes devastating neurological deficits, particularly through damage to fibers descending from the brain to the spinal ...

Development of an Alpha-synuclein Based Rat Model for Parkinson's Disease via Stereotactic Injection of a Recombinant Adeno-associated Viral Vector

Bir rekombinant adeno-ilişkili viral vektörün stereotaktik enjeksiyon yoluyla Parkinson Hastalığı için bir alfa-sinüklein Bazlı Fare Modeli Geliştirme

In order to study the molecular pathways of Parkinson&#39;s disease (PD) and to develop novel therapeutic strategies, scientific investigators rely on ...