Name: induction and monitoring of adoptive delayed-type hypersensitivity in rats
Uploaded: 2007-10-01T00:00:00
Description: Watch this Scientific Journal Video about Induction and Monitoring of Adoptive Delayed-Type Hypersensitivity in Rats at JoVE.com

<p>我们感谢为我们提供了GFP标记，卵清蛋白特定的T细胞在本议定书中使用的行博士亚历山大弗留葛尔（Martinsried，德国）。 </p>

<p class="jove_title"> 1。卵清蛋白特异性T淋巴细胞的刺激</p><p class="jove_content">休眠卵清蛋白特异性T细胞受到刺激照射Lewis大鼠胸腺细胞（30戈瑞）作为抗原提呈细胞（APCs）刺激中等，（准备的DMEM + PEN /链球菌/ L的过剩+ NEAA +的RPMI维生素的存在与卵清蛋白+ 2ME +大鼠血清+卵清蛋白，终浓度指出的材料清单。） </p><p class="jove_step">大鼠血清的制备</p><ol><li>我们从我们牺牲的大鼠胸腺捐助者的血清。 </li><li>大鼠深深氟烷吸入...

<ol>
	<li>Beeton, C., Barbaria, J., Devaux, J., Benoliel, A. -. M., Gola, M., Sabatier, J. -. M., Bernard, D., Crest, M., Beraud, E. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Selective+blocking+of+voltage-gated+K++channels+treats+experimental+autoimmune+encephalomyelitis+and+inhibits+T-cell+activation.">Selective blocking of voltage-gated K+ channels treats experimental autoimmune encephalomyelitis and inhibits T-cell activation.</a> <em style='font-style: italic'>J. Immunol</em>. , 166-936 (2001).</li><li>Beeton, C., Chandy, K. G. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Induction+and+monitoring+of+active+delayed+type+hypersensitivity+(DTH)in+rats.">Induction and monitoring of active delayed type hypersensitivity (DTH)in rats.</a> <em style='font-style: italic'>J Vis Exp</em>.  (5), (2007).</li></ol>

<p>抗原的挑战，也可以在其他网站，例如背部的皮肤（2）。不过，我们发现，具有挑战性的。在此视频中显示，使用耳的耳廓，允许DTH反应的最精确的测量。 </p>

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		<th>Material Name</th>
		<th>Type</th>
		<th>Company</th>
		<th>Catalogue Number</th>
		<th>Comment</th>
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<tr>
<td>DMEM</td>
<td>&nbsp;</td>
<td>Gibco/Invitrogen</td>
<td>12430-062</td>
<td>500ml</td>
</tr>
<tr>
<td>Sodium pyruvate</td>
<td>&nbsp;</td>
<td>Sigma</td>
<td>S8636</td>
<td>1 mM final, in Stimulation medium</td>
</tr>
<tr>
<td>Penicillin &#x2013; Streptomycin &#x2013; L-Glutamine</td>
<td>&nbsp;</td>
<td>Cambrex/BioWhittaker</td>
<td>17-718R</td>
<td>100 u/ml - 100 &micro;g/ml - 4 mM respectively, final, in Stimulation Medium </td>
</tr>
<tr>
<td>Non essential amino acids</td>
<td>&nbsp;</td>
<td>Sigma</td>
<td>R7131</td>
<td>NEAA, 1% final, in Stimulation Medium</td>
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<tr>
<td>RPMI vitamins</td>
<td>&nbsp;</td>
<td>Sigma</td>
<td>R7256</td>
<td>1% final, in Stimulation medium</td>
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<tr>
<td>beta-mercaptoethanol</td>
<td>&nbsp;</td>
<td>Sigma</td>
<td>M6250</td>
<td>2ME, 50 &micro;M final, in Stimulation Medium</td>
</tr>
<tr>
<td>Syngeneic rat serum</td>
<td>&nbsp;</td>
<td>&nbsp;</td>
<td>&nbsp;</td>
<td>1%  final. See explanation in Protocol.</td>
</tr>
<tr>
<td>Ovalbumin</td>
<td>&nbsp;</td>
<td>Sigma</td>
<td>A5503</td>
<td>10 &micro;g/ml final, in Stimulation Medium.</td>
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induction and monitoring of adoptive delayed-type hypersensitivity in rats

<p class="jove_content">迟发型超敏反应（DTH）是一个CCR7的效应记忆T淋巴细胞浸润针对其免疫系统已催芽的抗原注射部位介导的炎症反应。炎症反应的特点是由抗原挑战的部位的皮肤发红和肿胀。这是一个方便的模型，以确定在体内的免疫抑制剂的疗效。可诱发皮肤直接到户，抗原特异性T淋巴细胞过继转移或与抗原的主动免疫，并随后在给定面积的皮肤，诱发炎症反应的抗原皮内挑战。 DTH反应，可诱发各种抗原，如卵清蛋白，结核菌素，破伤风类毒素，或锁孔戚血蓝蛋白。这种反应也可以对自身抗原诱导，例如，髓鞘碱性蛋白（MBP）在大鼠实验性自身免疫性脑脊髓炎，多发性硬化症（1）动物模型的MBP诱导。在这里，我们将演示如何在Lewis大鼠诱发领养的DTH反应。我们将首先在体外刺激卵清蛋白特异性T细胞，注入这些天真大鼠腹腔激活细胞。允许细胞以平衡体内2天之后，我们将挑战同在一耳耳廓的卵清蛋白的老鼠，而另一只耳朵WIL收到生理盐水。炎症反应将是可见的3-72小时后，耳厚度测量的DTH严重性的迹象。</p>

Watch this Scientific Journal Video about Induction and Monitoring of Adoptive Delayed-Type Hypersensitivity in Rats at JoVE.com

Induction and Monitoring of Adoptive Delayed-Type Hypersensitivity in Rats

<p class="jove_content">迟发型超敏反应（DTH）是CCR7的效应记忆T（TEM）淋巴细胞介导的​​炎症反应。在这里，我们将演示如何激活抗原特异性的透射电镜细胞，诱导Lewis大鼠收养的DTH和监测的炎症反应。</p>

收养大鼠迟发型超敏反应的感应和监测

Delayed type hypersensitivity (DTH) is an inflammatory reaction mediated by CCR7- effector memory T lymphocytes that infiltrate the site of injection of ...

Research

JoVE Journal

Biology

Induction and Monitoring of Active Delayed Type Hypersensitivity (DTH) in Rats

Induction and Monitoring of Active Delayed Type Hypersensitivity DTH in Rats

大鼠主动迟发型超敏反应（DTH）的诱导和监控

科研

生物学

Imaging Effector Memory T cells in the Ear After Induction of Adoptive DTH

成像效应在耳朵上的记忆体T细胞诱导后收养的DTH

Delayed type hypersensitivity (DTH) is an immune reaction in which the main players are CCR7- effector / memory T lymphocytes. Here, we demonstrate a ...

Assay for Neural Induction in the Chick Embryo

在鸡胚神经诱导的含量为

The chick embryo is a valuable tool in the study of early embryonic development. Its transparency, accessibility and ease of manipulation, make it an ...

Changes in Mammary Gland Morphology and Breast Cancer Risk in Rats

变化在大鼠乳腺形态和患乳腺癌的风险

Studies in rodent models of breast cancer show that exposures to dietary/hormonal factors during the in utero and pubertal periods, when the mammary gland ...

Induction and Testing of Hypoxia in Cell Culture

在细胞培养中缺氧诱导和测试

Hypoxia is defined as the reduction or lack of oxygen in organs, tissues, or cells.  This decrease of oxygen tension can be due to a reduced supply in ...

Diagnostic Necropsy and Selected Tissue and Sample Collection in Rats and Mice

剖检诊断，并选择在大鼠和小鼠的组织和样品收集

There are multiple sample types that may be collected from a euthanized animal in order to help diagnose or discover infectious agents in an animal ...

Efficient Derivation of Human Cardiac Precursors and Cardiomyocytes from Pluripotent Human Embryonic Stem Cells with Small Molecule Induction

高效的推导人类心脏的前体和从多能干的小分子诱导人类胚胎干细胞的心肌细胞

To date, the lack of a suitable human cardiac cell source has been the major setback in regenerating the human myocardium, either by cell-based ...

Long-term Lethal Toxicity Test with the Crustacean <em>Artemia franciscana</em>

Long-term Lethal Toxicity Test with the Crustacean Artemia franciscana

致命的甲壳类动物长期毒性试验<em>卤虫franciscana</em

Our research activities target the use of biological methods for the evaluation of environmental quality, with particular reference to saltwater/brackish ...

Induction and Analysis of Epithelial to Mesenchymal Transition

诱导上皮和分析间质转化

Epithelial  to mesenchymal transition (EMT) is essential for proper morphogenesis during  development. Misregulation of this  process has been implicated ...

<em>In Vitro</em> Synthesis of Modified mRNA for Induction of Protein Expression in Human Cells

In Vitro Synthesis of Modified mRNA for Induction of Protein Expression in Human Cells

<em>在体外</em>合成在人体细胞修饰蛋白表达的mRNA的感应

The exogenous delivery of coding synthetic messenger RNA (mRNA) for induction of protein synthesis in desired cells has enormous potential in the fields ...