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JoVE Journal

Bioengineering

This content is Open Access.

Encapsulation of Cardiomyocytes in a Fibrin Hydrogel for Cardiac Tissue Engineering

23.9K Views

10:18 min

September 19th, 2011

September 19th, 2011

23,944 Views

0:05

Title

1:26

Neonatal Cardiomyocyte Isolation

3:45

Casting Fibrin Gel Constructs via Injection Molding

6:56

Contraction Force Testing

8:17

Representative Fibrin Construct Analyses

9:15

Conclusion

We describe the isolation of neonatal cardiomyocytes and the preparation of the cells for encapsulation in fibrin hydrogel constructs for tissue engineering. We describe methods for analyzing the tissue engineered myocardium after the culture period including active force generated upon electrical stimulation and cell viability and immunohistological staining.

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