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DOI :
10.3791/3273-v
•
10:47 min
October 28th, 2011
Chapters
0:05
Title
1:27
Media Preparation and Plate Coating
2:33
Passaging and Seeding Undifferentiated Human ES Cells
4:31
Neural Induction in Adhesive Culture with Retinoic Acid
5:42
Differentiation in Suspension Culture and Maturation to Neuronal Phenotype after Attachment
8:08
Direct Neuronal Differentiation of Feeder- and Serum- Free Pluripotent Human ES Cells WIth Retinoic Acid Induction
10:11
Conclusion
私たちは、人間の神経前駆細胞の大量供給と神経修復のための開発CNSにおける神経細胞の種類の導出を可能にする低分子化合物、と定義された条件の下で維持さ多能性ヒト胚性幹細胞から直接、神経芽細胞の誘導のためのプロトコルを確立している。
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