Name: implantation of a carotid cuff for triggering shear-stress induced atherosclerosis in mice
Uploaded: 2012-01-13T12:00:00
Description: Watch this Scientific Journal Video about Implantation of a Carotid Cuff for Triggering Shear-stress Induced Atherosclerosis in Mice at JoVE.com

Denne undersøgelse blev støttet delvist af Deutsche Forschungsgemeinschaft (DFG), projekt GZ PI 771/1-1; SFB 656 &quot;Cardiovascular Molecular Imaging,&quot; Münster, Tyskland (projekter C6, Z2, B3, og PM3), EU ekspertisenet &quot;Diagnostisk Molecular Imaging-dimi &quot;(WP 11.1 og 11.2). Undersøgelsen blev også finansieret en del af British Heart Foundation, England.

1. Forberedelse af shear stress modifier (manchet) <ol><li> Den forskydningsspænding modifier består af to langsgående halvdele af en cylinder med en kegle formet lumen. Den halve skaller er lavet af termoplastisk polyetherketone produceret af en plastik støbning procedure. De støbte elementer er sendt ud, mens du stadig forbundet til løberen. Derfor den halve skaller er nødt til at være skåret af før brug. Hver støbt indeholder halvskåle i forskellige størrelser lige fra 150 μm - 300 ...

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	<li>Chiu, J. J., Chien, S. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Effects+of+disturbed+flow+on+vascular+endothelium:+pathophysiological+basis+and+clinical+perspectives.">Effects of disturbed flow on vascular endothelium: pathophysiological basis and clinical perspectives.</a> Physiol. Rev. 91, 327-387 (2011).</li><li>Cunningham, K. S., Gotlieb, A. I. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=The+role+of+shear+stress+in+the+pathogenesis+of+atherosclerosis.">The role of shear stress in the pathogenesis of atherosclerosis.</a> Lab. Invest. 85, 9-23 (2005).</li><li>Ali, F., Zakkar, M., Karu, K., Lidington, E. A., Hamdulay, S. S., Boyle, J. J., Zloh, M., Bauer, A., Haskard, D. O., Evans, P. C., Mason, J. 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Imaging. 27, 1309-1318 (2009).</li><li>Stone, P. H., Coskun, A. U., Kinlay, S., Clark, M. E., Sonka, M., Wahle, A., Ilegbusi, O. J., Yeghiazarians, Y., Popma, J. J., Orav, J., Kuntz, R. E., Feldman, C. L. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Effect+of+endothelial+shear+stress+on+the+progression+of+coronary+artery+disease,+vascular+remodeling,+and+in-stent+restenosis+in+humans:+in+vivo+6-month+follow-up+study.">Effect of endothelial shear stress on the progression of coronary artery disease, vascular remodeling, and in-stent restenosis in humans: in vivo 6-month follow-up study.</a> Circulation. 108, 438-444 (2003).</li><li>Pedersen, E. M., Oyre, S., Agerbaek, M., Kristensen, I. B., Ringgaard, S., Boesiger, P., Paaske, W. 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W., Rezvan, A., Suo, J., Budzyn, K., Llanos, A., Harrison, D., Giddens, D., Jo, H. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Partial+carotid+ligation+is+a+model+of+acutely+induced+disturbed+flow,+leading+to+rapid+endothelial+dysfunction+and+atherosclerosis.">Partial carotid ligation is a model of acutely induced disturbed flow, leading to rapid endothelial dysfunction and atherosclerosis.</a> Am. J. Physiol. Heart. Circ. Physiol. 297, 1535-1543 (2009).</li><li>Cheng, C., van Haperen, R., de Waard, M., van Damme, L. C., Tempel, D., Hanemaaijer, L., van Cappellen, G. W., Bos, J., Slager, C. J., Duncker, D. J., van der Steen, A. F., de Crom, R., Krams, R. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Shear+stress+affects+the+intracellular+distribution+of+eNOS:+direct+demonstration+by+a+novel+in+vivo+technique.">Shear stress affects the intracellular distribution of eNOS: direct demonstration by a novel in vivo technique.</a> Blood. 106, 3691-3698 (2005).</li><li>Cheng, C., Tempel, D., van Haperen, R., van der Baan, A., Grosveld, F., Daemen, M. J., Krams, R., de Crom, R. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Atherosclerotic+lesion+size+and+vulnerability+are+determined+by+patterns+of+fluid+shear+stress.">Atherosclerotic lesion size and vulnerability are determined by patterns of fluid shear stress.</a> Circulation. 113, 2744-2753 (2006).</li><li>van Bochove, G. S., Straathof, R., Krams, R., Nicolay, K., Strijkers, G. J. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=MRI-determined+carotid+artery+flow+velocities+and+wall+shear+stress+in+a+mouse+model+of+vulnerable+and+stable+atherosclerotic+plaque.">MRI-determined carotid artery flow velocities and wall shear stress in a mouse model of vulnerable and stable atherosclerotic plaque.</a> MAGMA. 23, 77-84 (2010).</li><li>Cuhlmann, S., Van der Heiden, K., Saliba, D., Tremoleda, J. L., Khalil, M., Zakkar, M., Chaudhury, H., Luong, L. A., Mason, J. C., Udalova, I., Gsell, W., Jones, H., Haskard, D. O., Krams, R., Evans, P. 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For at minimere eksperimentelle variation anbefales det at arbejde med dyr af næsten samme alder og med samme diæt historie. En nylig offentliggjort undersøgelse viser, at shear stress modifier anvendes i vildtype mus kan være en god model til undersøgelse af endotel dysfunktion og begyndelsen af inflammatoriske reaktioner fremkaldt af ændrede flow dynamik 13. Men for den undersøgelse af aterosklerotiske plaque udvikling transgene hyperlipidæmiske musemodeller (f.eks ApoE knockout mus) er påkrævet. ...

<table border="1"><tbody><tr><td> Navn </td><td> Firma </td><td> Kommentarer </td></tr><tr><td> Shear stress modifier (polyetherketone) </td><td> Promolding BV <a href="http://promolding.nl">http://promolding.nl</a> </td><td> De kaster bliver sendt ud, mens der stadig er forbundet med løberen. Således er de enkelte elementer er nødt til at være skåret af før brug. </td></tr></tbody></table>

implantation of a carotid cuff for triggering shear-stress induced atherosclerosis in mice

Det er almindeligt accepteret, at ændringer i vaskulær forskydningsspænding udløse udtryk for inflammatoriske gener i endotelceller og dermed fremkalde åreforkalkning (revideret i 1 og 2). Rolle shear stress er blevet grundigt undersøgt in vitro undersøge indflydelse af flow dynamik på dyrkede endotelceller 1,3,4 og in vivo i større dyr og mennesker 1,5,6,7,8. Men, meget reproducerbar små dyremodeller tillader systematisk undersøgelse af indflydelsen af ​​shear stress på plak udvikling er sjældne. For nylig introducerede Nam et al. 9 en musemodel, hvor ligatur af filialer af halspulsåren oprettes et område med lav og oscillerende flow. Selv om denne model medfører endotel dysfunktion og hurtig dannelse af aterosklerotiske læsioner i hyperlipidæmiske mus, kan det ikke udelukkes, at den observerede inflammatoriske reaktion er, i hvert fald delvist, en følge of endotel-og / eller fartøj skade på grund af ligatur. For at undgå sådanne begrænsninger, har en forskydningsspænding ændre manchet blevet udviklet baseret på beregnede fluid dynamik, hvis kegleformede indre lumen blev udvalgt til at lave definerede områder med lav, høj og oscillerende shear stress inden for det fælles halspulsåren 10. Ved at anvende denne model i Apolipoprotein E (ApoE) knockout mus fodret med et højt kolesteroltal vestlige typen kost, vaskulære læsioner udvikle opstrøms og nedstrøms fra manchetten. Deres fænotype er korreleret med de regionale flow dynamik 11 som bekræftes af in vivo Magnetic Resonance Imaging (MRI) 12: Lav og laminar forskydningsspænding opstrøms af manchetten forårsager dannelsen af omfattende plaques af en mere sårbar fænotype, mens oscillerende forskydningsspænding nedstrøms manchetten inducerer stabil aterosklerotiske læsioner 11. I disse regioner med høj shear stress og høj laminar flow i manchet,typisk ikke aterosklerotiske plaques overholdes. Konklusionen er, at den forskydningsspænding antireumatiske manchet procedure en pålidelig kirurgisk tilgang til at producere fænotypisk forskellige aterosklerotiske læsioner i ApoE-mangel mus.

Watch this Scientific Journal Video about Implantation of a Carotid Cuff for Triggering Shear-stress Induced Atherosclerosis in Mice at JoVE.com

Implantation of a Carotid Cuff for Triggering Shear-stress Induced Atherosclerosis in Mice

Den snærende manchet præsenteres i denne artikel er designet til at fremkalde åreforkalkning i murine fælles halspulsåren. På grund af den koniske form af dens indre lumen den implanterede manchetten genererer veldefinerede områder med lav, høj og oscillerende shear stress udløser udvikling af aterosklerotiske læsioner af forskellige inflammatoriske fænotyper.

Implantation af en carotis Cuff til udløsning af shear-stress Induced Åreforkalkning i Mus

It is widely accepted that alterations in vascular shear stress trigger the expression of inflammatory genes in endothelial cells and thereby induce ...

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