Name: a contusive model of unilateral cervical spinal cord injury using the infinite horizon impactor
Uploaded: 2012-07-24T12:00:00
Description: Watch this Scientific Journal Video about A Contusive Model of Unilateral Cervical Spinal Cord Injury Using the Infinite Horizon Impactor at JoVE.com

1. Opsætning: Ramme og klemme er konstrueret til at holde dyr <ol><li> Rammen og klemme til at holde dyret var specialdesignet til at rumme den Uendelige Horizon (IH) Rygmarvsskader Impactor. </li><li> Bunden af rammen er en aluminium platform skåret i de følgende dimensioner (30,2 cm x 20,3 cm x 1,3 cm) med henblik på at passe ind i tabel guide beslaget, der kommer standard med IH enheden (figur 1A). </li><li> Fire Flexaframe support trædeflader (Fisher Scientific, Toronto, ON) er fastgjort til ...

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I dette papir, beskriver vi en cervikal ensidig kontusion model under anvendelse af Infinite Horizon (IH) slaglegeme ved en kraft på 150 kdyn, en vinkel på 22,5 ° fra vertikal, og en lateral formål 1,4 mm fra midterlinien. Med disse indstillinger, var vi i stand til at producere vedvarende adfærdsmæssige underskud i ipsilaterale forben med parenkymale forstyrrelser indeholdt stort set til den ipsilaterale side, hvor det fremgik, at betydelig skade sket på de regioner, hvor de rubrospinal, reticulospinal, vestibul...

<table border="1"><tbody><tr><td> Navn udstyr </td><td> Firma </td><td> Katalog nummer </td><td> Kommentarer </td></tr><tr><td> Uendelig Horizon Impactor </td><td> Precision Systems og instrumentering </td><td> IH-0400 </td><td></td></tr><tr><td> Aluminiummetal plade </td><td> Metalsupermarlets.com </td><td> APT6061/500 </td><td></td></tr><tr><td> Flexaframe support trædeflader </td><td> Fishers Videnskabelig </td><td> 1466625Q </td><td></td></tr><tr><td> Flexaframe støttestænger </td><td> Fishers Videnskabelig </td><td> 1466610GQ </td><td></td></tr><tr><td> Flexaframe Support Stik </td><td> Fishers Videnskabelig </td><td> 1466620Q </td><td></td></tr><tr><td> Klemme 1 </td><td> Skræddersyet </td><td></td><td> Choo et al., 2009 </td></tr><tr><td> Metal indehavere </td><td> Skræddersyet </td><td></td><td> Se ovenfor </td></tr><tr><td> Impactor tip </td><td> Skræddersyet </td><td></td><td> Diameter: 1,15 mm </td></tr><tr><td> Stereotaktisk ramme </td><td> David Kopf Instruments </td><td> Model 900 </td><td></td></tr><tr><td> Cylinder Level </td><td> Yijia VÆRKTØJER </td><td> YJ-SL0620 </td><td></td></tr><tr><td> Mikroskop </td><td> Leica </td><td></td><td> Model #: MZ8 </td></tr><tr><td> Laboratory saks Jack </td><td> VWR </td><td> 12620-902 </td><td></td></tr></tbody></table>

a contusive model of unilateral cervical spinal cord injury using the infinite horizon impactor

Mens størstedelen af ​​humane rygmarvsskadede forekommer i den cervikale rygmarv, at langt størstedelen af ​​laboratorieforskning anvender dyremodeller for rygmarvsskade (SCI), hvor den thorakale rygmarven er skadet. Derudover, fordi de fleste mennesker ledning skader opstår som følge af stump, ikke-penetrerende traumer (f.eks motorkøretøjsulykke, sportslige skade), hvor rygmarven er voldsomt ramt af fordrevne knogle eller blødt væv, de fleste af SCI forskere er af den opfattelse at de fleste klinisk relevante skadesindikatorer modeller er dem, hvor rygmarven hurtigt contused. 1 derfor et vigtigt skridt i den prækliniske evaluering af nye behandlinger på vej til menneskelig oversættelse er en vurdering af deres effekt i en model af kontusion SCI inden Den cervikale rygmarv. Her beskriver vi de tekniske aspekter og deraf følgende anatomiske og adfærdsmæssige resultater af en ensidig contusive model af livmoderhalskræft SCI, der beskæftigerInfinite Horizon rygmarvsskade attrap. Sprague Dawley-rotter gennemgik en venstre-sidet ensidig laminektomi ved C5. For at optimere reproducerbarheden af ​​de biomekaniske, funktionelle og histologiske resultater af skaden model, vi contused de rygmarve med en slagkraft på 150 kdyn, en indvirkning bane på 22,5 ° (dyr roteret ved 22,5 °), og en effekt placering off af midterlinjen på 1,4 mm. Funktionel opsving blev vurderet ved hjælp af cylinderen opdræt testen, vandret stige test, grooming test og modificeret Montoya har trappe test for op til 6 uger, hvorefter rygmarve blev vurderet histologisk for hvid og grå stof sparsom. Skaden model præsenteret her bibringer konsekvent og reproducerbar biomekaniske kræfter til rygmarven, et vigtigt træk ved en eksperimentel SCI model. Dette resulterer i diskrete histologisk skade laterale halvdel af rygmarven, der hovedsagelig indeholdt til tHan ipsilaterale side af skade. Skaden tåles godt af dyrene, men ikke resulterer i funktionelle underskud i forben, som er væsentlige og vedvarende i ugerne efter skaden. Den cervikale ensidige skade model præsenteres her, kan være en ressource for forskere, der ønsker at vurdere potentielt lovende behandlinger forud for menneskelig oversættelse.

Watch this Scientific Journal Video about A Contusive Model of Unilateral Cervical Spinal Cord Injury Using the Infinite Horizon Impactor at JoVE.com

A Contusive Model of Unilateral Cervical Spinal Cord Injury Using the Infinite Horizon Impactor

En pålidelig og reproducerbar måde at frembringe en cervikal ensidig rygmarvsskade hjælp af uendelige Horizon slaglegemet beskrevet. Fremgangsmåden drager fordel af et designet ramme og fastspænde at stabilisere rygsøjlen. Den standardiserede procedure og biomekaniske skade parametre resultere i tilstrækkelig og vedvarende skader.

En Contusive Model af Unilateral Cervikal Rygmarvsskader Brug af Uendelige Horizon Impactor

While the majority of human spinal cord injuries occur in the cervical spinal cord, the vast majority of laboratory research employs animal models of ...

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Spinal cord injury (SCI) impairs sensory systems causing allodynia1-8. To identify cellular and molecular causes of allodynia, sensitive and valid sensory ...

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Use of genetically modified mice enhances our  understanding of molecular mechanisms underlying several neurological disorders  such as a spinal cord ...

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The translational potential of novel treatments should be investigated in severe spinal cord injury (SCI) contusion models. A detailed methodology is ...

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Clinically-relevant animal cervical spinal cord injury (SCI) models are essential for developing and testing potential therapies; however, producing ...

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En murin model af cervikal Rygmarvsskader til Study Post-lesional Respiratory Neuroplasticitet

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High thoracic or cervical spinal cord injury (SCI) can lead to cardiovascular dysfunction. To monitor cardiovascular parameters, we implanted a catheter ...

Synergetic Use of Neural Precursor Cells and Self-assembling Peptides in Experimental Cervical Spinal Cord Injury

Fordelagtig samordning af neurale precursorceller og selvsamlende peptider i Experimental Cervikal Rygmarvsskader

Spinal cord injuries (SCI) cause serious neurological impairment and psychological, economic, and social consequences for patients and their families. ...

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Spinal cord injury is a devastating clinical condition, characterized by a complex of neurological dysfunctions. Animal models of spinal cord injury can ...

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Compression injuries of the murine spinal cord are valuable animal models for the study of spinal cord injury (SCI) and spinal regenerative therapy. The ...