Name: a contusive model of unilateral cervical spinal cord injury using the infinite horizon impactor
Uploaded: 2012-07-24T12:00:00
Description: Watch this Scientific Journal Video about A Contusive Model of Unilateral Cervical Spinal Cord Injury Using the Infinite Horizon Impactor at JoVE.com

1. फ़्रेम और पशु होल्डिंग के लिए दबाना डिजाइन: सेट अप <ol><li> फ्रेम और दबाना करने के लिए पशु पकड़ कस्टम (IH) अनंत क्षितिज स्पाइनल कॉर्ड चोट impactor को समायोजित डिजाइन किया गया था. </li><li> फ्रेम के आधार पर एक एल्यूमीनियम...

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इस पत्र में, हम एक गर्भाशय ग्रीवा के एकतरफा कुचलन 150 kdyn, 22.5 डिग्री के एक बंद ऊर्ध्वाधर कोण, और midline से 1.4 मिमी की एक पार्श्व उद्देश्य के बल पर अनंत क्षितिज (IH) impactor मॉडल का उपयोग कर का वर्णन. इन सेटिंग्स के साथ, हम parenc...

<table border="1"><tbody><tr><td> उपकरणों के नाम </td><td> कंपनी </td><td> सूचीपत्र संख्या </td><td> टिप्पणियाँ </td></tr><tr><td> अनंत क्षितिज impactor </td><td> प्रेसिजन सिस्टम और इंस्ट्रुमेंटेशन </td><td> IH - 0400 </td><td></td></tr><tr><td> एल्यूमिनियम धातु शीट </td><td> Metalsupermarlets.com </td><td> APT6061/500 </td><td></td></tr><tr><td> Flexaframe समर्थन पैर प्लेटें </td><td> फिशर्स वैज्ञानिक </td><td> 1466625Q </td><td></td></tr><tr><td> Flexaframe समर्थन छड़ </td><td> फिशर्स वैज्ञानिक </td><td> 1466610GQ </td><td></td></tr><tr><td> Flexaframe समर्थन connectors </td><td> फिशर्स वैज्ञानिक </td><td> 1466620Q </td><td></td></tr><tr><td> एक क्लैंप </td><td> कस्टम बनाया </td><td></td><td> चू एट अल., 2009 </td></tr><tr><td> धातु धारकों </td><td> कस्टम बनाया </td><td></td><td> ऊपर देखें </td></tr><tr><td> Impactor टिप </td><td> कस्टम बनाया </td><td></td><td> व्यास: 1.15 मिमी </td></tr><tr><td> Stereotaxic फ्रेम </td><td> दाऊद KOPF उपकरण </td><td> 900 मॉडल </td><td></td></tr><tr><td> सिलेंडर स्तर </td><td> YIJIA उपकरण </td><td> YJ-SL0620 </td><td></td></tr><tr><td> माइक्रोस्कोप </td><td> Leica </td><td></td><td> मॉडल #: MZ8 </td></tr><tr><td> प्रयोगशाला कैंची जैक </td><td> VWR </td><td> 12620-902 </td><td></td></tr></tbody></table>

a contusive model of unilateral cervical spinal cord injury using the infinite horizon impactor

While the majority of human spinal cord injuries occur in the cervical spinal cord, the vast majority of laboratory research employs animal models of spinal cord injury (SCI) in which the thoracic spinal cord is injured. Additionally, because most human cord injuries occur as the result of blunt, non-penetrating trauma (e.g. motor vehicle accident, sporting injury) where the spinal cord is violently struck by displaced bone or soft tissues, the majority of SCI researchers are of the opinion that the most clinically relevant injury models are those in which the spinal cord is rapidly contused.1 Therefore, an important step in the preclinical evaluation of novel treatments on their way to human translation is an assessment of their efficacy in a model of contusion SCI within the cervical spinal cord. Here, we describe the technical aspects and resultant anatomical and behavioral outcomes of an unilateral contusive model of cervical SCI that employs the Infinite Horizon spinal cord injury impactor.
Sprague Dawley rats underwent a left-sided unilateral laminectomy at C5. To optimize the reproducibility of the biomechanical, functional, and histological outcomes of the injury model, we contused the spinal cords using an impact force of 150 kdyn, an impact trajectory of 22.5&deg; (animals rotated at 22.5&deg;), and an impact location off of midline of 1.4 mm. Functional recovery was assessed using the cylinder rearing test, horizontal ladder test, grooming test and modified Montoya's staircase test for up to 6 weeks, after which the spinal cords were evaluated histologically for white and grey matter sparing.
The injury model presented here imparts consistent and reproducible biomechanical forces to the spinal cord, an important feature of any experimental SCI model. This results in discrete histological damage to the lateral half of the spinal cord which is largely contained to the ipsilateral side of injury. The injury is well tolerated by the animals, but does result in functional deficits of the forelimb that are significant and sustained in the weeks following injury. The cervical unilateral injury model presented here may be a resource to researchers who wish to evaluate potentially promising therapies prior to human translation.

Watch this Scientific Journal Video about A Contusive Model of Unilateral Cervical Spinal Cord Injury Using the Infinite Horizon Impactor at JoVE.com

A Contusive Model of Unilateral Cervical Spinal Cord Injury Using the Infinite Horizon Impactor

एक विश्वसनीय और repeatable एक गर्भाशय ग्रीवा एकतरफा रीढ़ की हड्डी में चोट अनंत क्षितिज impactor का उपयोग कर उत्पादन में वर्णित है. विधि एक कस्टम डिजाइन फ्रेम के लाभ लेता है और रीढ़ को स्थिर करने के लिए दबाना है. मानकीकृत प्रक्रिया और पर्याप्त और घायल में biomechanical चोट मापदंडों परिणाम है.

में एकतरफा सरवाइकल स्पाइनल कॉर्ड चोट की एक Contusive मॉडल अनंत क्षितिज impactor

While the majority of human spinal cord injuries occur in the cervical spinal cord, the vast majority of laboratory research employs animal models of ...

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