Name: a contusive model of unilateral cervical spinal cord injury using the infinite horizon impactor
Uploaded: 2012-07-24T12:00:00
Description: Watch this Scientific Journal Video about A Contusive Model of Unilateral Cervical Spinal Cord Injury Using the Infinite Horizon Impactor at JoVE.com

1。動物を保持するためのフレームとクランプデザイン：セットアップ<ol><li>動物を保持するフレームとクランプは無限の地平線（IH）脊髄損傷のインパクを収容するためにカスタム設計されました。 </li><li>フレームのベースは、IH機器（ 図1A）に標準装備されているテーブルのガイドブラケットに収まるようにするために以下の寸法（30.2センチ×20.3センチメートル×1.3センチ）...

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本稿では、150 kdyn、垂直のオフ22.5°の角度と、正中線から1.4 mmの横目的の力で無限の地平線（IH）インパクターを用いて子宮頸部片側挫傷モデルを説明します。これらの設定で、我々は、そのかなりのダメージが赤核脊髄、網様体脊髄、前庭と皮質路がすることが期待される地域に発生した登場し、主に同側の側に含まれている実質の混乱、と同側の前肢の持続的行動障害を生成することがで...

<table border="1"><tbody><tr><td> 機器の名前 </td><td> 会社 </td><td> カタログ番号 </td><td> コメント </td></tr><tr><td>無限の地平線インパク</td><td>精度システムと計装</td><td> IH-0400 </td><td></td></tr><tr><td>アルミニウム金属シート</td><td> Metalsupermarlets.com </td><td> APT6061/500 </td><td></td></tr><tr><td> Flexaframeサポートフットプレート</td><td>フィッシャーズ科学</td><td> 1466625Q </td><td></td></tr><tr><td> Flexaframeサポートロッド</td><td>フィッシャーズ科学</td><td> 1466610GQ </td><td></td></tr><tr><td> Flexaframeサポートコネクタ</td><td>フィッシャーズ科学</td><td> 1466620Q </td><td></td></tr><tr><td>クランプ1 </td><td>カスタムメイド</td><td></td><td> Choo ら 。2009年</td></tr><tr><td>メタルホルダー</td><td>カスタムメイド</td><td></td><td>上記参照</td></tr><tr><td>インパクタ先端</td><td>カスタムメイド</td><td></td><td>直径：1.15ミリメートル</td></tr><tr><td>定位フレーム</td><td>デビッド·Kopfは楽器</td><td>モデル900 </td><td></td></tr><tr><td>シリンダーレベル</td><td> YIJIA TOOLS </td><td> YJ-SL0620 </td><td></td></tr><tr><td>顕微鏡</td><td>ライカ</td><td></td><td>モデル番号：MZ8 </td></tr><tr><td>実験室シザージャッキ</td><td> VWR </td><td> 12620-902 </td><td></td></tr></tbody></table>

a contusive model of unilateral cervical spinal cord injury using the infinite horizon impactor

人間の脊髄損傷の大部分が脊髄に発生しながら、実験室での研究の大半は胸髄が負傷されている脊髄損傷（SCI）の動物モデルを採用しています。ほとんどの人間の脊髄損傷は脊髄が激しく避難骨や軟部組織に打たれ鈍、非貫通外傷（例えば、自動車事故、スポーツ傷害）の結果として発生するため、さらに、SCI研究者の大多数は意見である最も臨床的に関連する傷害モデルでは、脊髄が急速にcontusedであるものであること1。したがって、人間の翻訳に向かう上での新規治療法の臨床評価の重要なステップは、内挫傷SCIのモデルでの有効性の評価です。頸髄。ここでは、採用して子宮頸部SCIの一方的な打撲のモデルの技術的な側面、その結果、解剖学的および行動の成果を記述する無限の地平線脊髄損傷インパクター。 Sprague DawleyラットはC5で左サイド片側椎弓切除術を施行した。損傷モデルの、生体力学的機能、および組織成果の再現性を最適化するために、我々は150 kdyn、22.5°（動物は22.5°で回転）の影響の軌道、インパクトの位置からの衝撃力を用いて脊髄をcontused 1.4 mmの正中線の。機能回復は、脊髄の白と灰白質の温存のために組織学的に評価した後、6週間までのシリンダ飼育試験、水平方向のラダーテスト、グルーミングテストおよび変更されたモントーヤの階段試験を用いて評価した。 ここに提示傷害モデルでは、脊髄への実験的なSCIモデルの重要な機能を一貫して再現性の生体力学的な力を与える。離散的な組織の損傷で、この結果、主にtに含まれている脊髄の外側半分にけがの彼は同側。怪我はよく動物の忍容性が、損傷後数週間で重要かつ持続的である前肢の機能障害の結果をしています。ここに提示頚部片側損傷モデルは、人間の翻訳の前に潜在的に有望な治療法を評価する研究者へのリソースかもしれません。

Watch this Scientific Journal Video about A Contusive Model of Unilateral Cervical Spinal Cord Injury Using the Infinite Horizon Impactor at JoVE.com

A Contusive Model of Unilateral Cervical Spinal Cord Injury Using the Infinite Horizon Impactor

無限の地平線インパクターを用いて頚一方的な脊髄損傷を生成するための信頼性と再現性のある方法が記載されています。メソッドは、カスタム設計されたフレームを活用し、背骨を安定させるためにクランプします。標準化された手順と十分かつ持続的な傷害の生体力学的傷害のパラメータ結果。

無限の地平線インパクターを用いた片側頚髄損傷の打撲のモデル

While the majority of human spinal cord injuries occur in the cervical spinal cord, the vast majority of laboratory research employs animal models of ...

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ラット脊髄損傷後の急性および慢性の触覚官能検査

Spinal cord injury (SCI) impairs sensory systems causing allodynia1-8. To identify cellular and molecular causes of allodynia, sensitive and valid sensory ...

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マウスで制御子宮裂傷の傷害

Use of genetically modified mice enhances our  understanding of molecular mechanisms underlying several neurological disorders  such as a spinal cord ...

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ラットにおける重度の脊髄損傷挫傷モデル

The translational potential of novel treatments should be investigated in severe spinal cord injury (SCI) contusion models. A detailed methodology is ...

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挫傷、脊髄損傷の新規な製造脊椎安定化法

Clinically-relevant animal cervical spinal cord injury (SCI) models are essential for developing and testing potential therapies; however, producing ...

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ポスト病変呼吸神経可塑性を研究するための子宮頸脊髄損傷のマウスモデル

A cervical spinal cord injury induces permanent paralysis, and often leads to respiratory distress. To date, no efficient therapeutics have been developed ...

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外傷性脳損傷のための制御皮質衝撃モデル

Every year over a million Americans suffer a traumatic brain injury (TBI).  Combined with the incidence of TBIs worldwide, the physical, emotional, ...

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ラジオ遠隔測定システムは、脊髄離断および胚性神経細胞移植片幹ラットの心臓血管機能を監視する

High thoracic or cervical spinal cord injury (SCI) can lead to cardiovascular dysfunction. To monitor cardiovascular parameters, we implanted a catheter ...

Synergetic Use of Neural Precursor Cells and Self-assembling Peptides in Experimental Cervical Spinal Cord Injury

実験的子宮頸脊髄損傷における神経前駆細胞と自己組織化ペプチドの相乗使用

Spinal cord injuries (SCI) cause serious neurological impairment and psychological, economic, and social consequences for patients and their families. ...

Neural Stem Cell Transplantation in Experimental Contusive Model of Spinal Cord Injury

脊髄損傷の実験的挫傷モデルにおける神経幹細胞移植

Spinal cord injury is a devastating clinical condition, characterized by a complex of neurological dysfunctions. Animal models of spinal cord injury can ...

Calibrated Forceps Model of Spinal Cord Compression Injury

脊髄圧縮傷害の校正済み鉗子モデル

Compression injuries of the murine spinal cord are valuable animal models for the study of spinal cord injury (SCI) and spinal regenerative therapy. The ...