Name: in situ hybridization for the precise localization of transcripts in plants
Uploaded: 2011-11-23T13:00:00
Duration: 12 min 15 s
Description: Watch this Scientific Journal Video about In Situ Hybridization for the Precise Localization of Transcripts in Plants at JoVE.com

在实验室吨的研究是由国家科学基金会（DBI - 0820610和IOS - 1022102）和纽约州卫生署（NYSTEM C024308）的赠款支持。 CM收到来自西班牙教育和科学部（2007-0937）和基金会拉斐尔 - 皮诺，西班牙部博士后。

<ol>
	<li>Rensink, W. A., Buell, C. R. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Microarray+expression+profiling+resources+for+plant+genomics.">Microarray expression profiling resources for plant genomics.</a> Trends. Plant. Sci. 10, 603-609 (2005).</li><li>Li, P., Ponnala, L., Gandotra, N., Wang, L., Si, Y., Tausta, S. L., Kebrom, T. H., Provart, N., Patel, R., Myers, C. R. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=The+developmental+dynamics+of+the+maize+leaf+transcriptome.">The developmental dynamics of the maize leaf transcriptome.</a> Nat. Genet. 42, 1060-1067 (2010).</li><li>Lister, R., O'Malley, R. C., Tonti-Filippini, J., Gregory, B. D., Berry, C. C., Millar, A. H., Ecker, J. R. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Highly+integrated+single-base+resolution+maps+of+the+epigenome+in+Arabidopsis.">Highly integrated single-base resolution maps of the epigenome in Arabidopsis.</a> Cell. 133, 523-536 (2008).</li><li>Brady, S. M., Orlando, D. A., Lee, J. Y., Wang, J. Y., Koch, J., Dinneny, J. R., Mace, D., Ohler, U., Benfey, P. N. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=A+high-resolution+root+spatiotemporal+map+reveals+dominant+expression+patterns.">A high-resolution root spatiotemporal map reveals dominant expression patterns.</a> Science. 318, 801-806 (2007).</li><li>Brooks, L., Strable, J., Zhang, X., Ohtsu, K., Zhou, R., Sarkar, A., Hargreaves, S., Elshire, R. J., Eudy, D., Pawlowska, T., Ware, D., Janick-Buckner, D. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Microdissection+of+shoot+meristem+functional+domains.">Microdissection of shoot meristem functional domains.</a> PLoS. Genet. 5, e1000476-e1000476 (2009).</li><li>Galbraith, D. W., Birnbaum, K. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Global+studies+of+cell+type-specific+gene+expression+in+plants.">Global studies of cell type-specific gene expression in plants.</a> Annu. Rev. Plant. Biol. 57, 451-475 (2006).</li><li>Ohtsu, K., Smith, M., Emrich, S., Borsuk, L., Zhou, R., Chen, T., Zhang, X., Timmermans, M., Beck, J., Buckner, B., Janick-Buckner, D., Nettleton, D., Scanlon, M., Schnable, P. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Global+gene+expression+analysis+of+the+shoot+apical+meristem+of+maize+(Zea+mays+L.).">Global gene expression analysis of the shoot apical meristem of maize (Zea mays L.).</a> Plant. J. 52, 391-404 (2007).</li><li>Juarez, M. T., Kui, J. S., Thomas, J., Heller, B. A., Timmermans, M. C. P. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=microRNA-mediated+repression+of+rolled+leaf1+specifies+maize+leaf+polarity.">microRNA-mediated repression of rolled leaf1 specifies maize leaf polarity.</a> Nature. 428, 84-88 (2004).</li><li>Kramer, E. M., Irish, V. F. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Evolution+of+genetic+mechanisms+controlling+petal+development.">Evolution of genetic mechanisms controlling petal development.</a> Nature. 399, 144-148 (1999).</li><li>Stuurman, J., Jäggi, F., Kuhlemeier, C. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Shoot+meristem+maintenance+is+controlled+by+a+GRAS-gene+mediated+signal+from+differentiating+cells.">Shoot meristem maintenance is controlled by a GRAS-gene mediated signal from differentiating cells.</a> Genes Dev. 16, 2213-2218 (2002).</li><li>Kim, M., McCormick, S., Timmermans, M., Sinha, N. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=The+expression+domain+of+PHANTASTICA+determines+leaflet+placement+in+compound+leaves.">The expression domain of PHANTASTICA determines leaflet placement in compound leaves.</a> Nature. 424, 438-443 (2003).</li><li>Kramer, E. M., Holappa, L., Gould, B., Jaramillo, M. A., Setnikov, D., Santiago, P. M. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Elaboration+of+B+gene+function+to+include+the+identity+of+novel+floral+organs+in+the+lower+eudicot+Aquilegia.">Elaboration of B gene function to include the identity of novel floral organs in the lower eudicot Aquilegia.</a> Plant Cell. 19, 750-766 (2007).</li><li>Lippman, Z. B., Cohen, O., Alvarez, J. P., Abu-Abied, M., Pekker, I., Paran, I., Eshed, Y., Zamir, D. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=The+making+of+a+compound+inflorescence+in+tomato+and+related+nightshades.">The making of a compound inflorescence in tomato and related nightshades.</a> PLoS Biol. 6, e288-e288 (2008).</li><li>Kidner, C., Timmermans, M. C. P. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=In+situ+hybridization+as+a+tool+to+study+the+role+of+microRNAs+in+plant+development.">In situ hybridization as a tool to study the role of microRNAs in plant development.</a> Methods. Mol. Biol. 342, 159-179 (2006).</li><li>Jackson, D. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Double+labeling+of+KNOTTED1+mRNA+and+protein+reveals+multiple+potential+sites+of+protein+trafficking+in+the+shoot.">Double labeling of KNOTTED1 mRNA and protein reveals multiple potential sites of protein trafficking in the shoot.</a> 129, 1423-1429 (2002).</li><li>Jensen, W. A. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=.">.</a> Botanical Histochemistry. , 408-408 (1962).</li><li>Jackson, D., Bowles, D. J., Gurr, S. J., McPherson, M. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=In+situ+hybridization+in+plants.">In situ hybridization in plants.</a> Molecular Plant Pathology: A Practical Approach. , 163-174 (1991).</li><li>Chitwood, D. H., Nogueira, F. T., Howell, M. o. n. t. g. o. m. e. r. y., Carrington, T. A., C, J., Timmermans, M. C. P. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Pattern+formation+via+small+RNA+mobility.">Pattern formation via small RNA mobility.</a> Genes. Dev. 23, 549-554 (2009).</li><li>Piette, D., Hendrickx, M., Willems, E., Kemp, C. R., Leyns, L. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=An+optimized+procedure+for+whole-mount+in+situ+hybridization+on+mouse+embryos+and+embryoid+bodies.">An optimized procedure for whole-mount in situ hybridization on mouse embryos and embryoid bodies.</a> Nat. Protoc. 3, 1194-1201 (2008).</li><li>Blilou, I., Xu, J., Wildwater, M., Willemsen, V., Paponov, I., Friml, J., Heidstra, R., Aida, M., Palme, K., Scheres, B. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=The+PIN+auxin+efflux+facilitator+network+controls+growth+and+patterning+in+Arabidopsis+roots.">The PIN auxin efflux facilitator network controls growth and patterning in Arabidopsis roots.</a> Nature. 433, 39-44 (2005).</li><li>Jackson, D., Hake, S. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Control+of+phyllotaxy+in+maize+by+the+abphyl1+gene.">Control of phyllotaxy in maize by the abphyl1 gene.</a> Development. , 126-315 (1999).</li><li>Chuck, G., Whipple, C., Jackson, D., Hake, S. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=The+maize+SBP-box+transcription+factor+encoded+by+tasselsheath4+regulates+bract+development+and+the+establishment+of+meristem+boundaries.">The maize SBP-box transcription factor encoded by tasselsheath4 regulates bract development and the establishment of meristem boundaries.</a> Development. 137, 1243-1250 (2010).</li><li>Long, J. A., Barton, M. K. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=The+development+of+apical+embryonic+pattern+in+Arabidopsis.">The development of apical embryonic pattern in Arabidopsis.</a> Development. 125, 3027-3035 (1998).</li><li>Nogueira, F. T., Chitwood, D. H., Madi, S., Ohtsu, K., Schnable, P. S., Scanlon, M. J., Timmermans, M. C. P. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Regulation+of+small+RNA+accumulation+in+the+maize+shoot+apex.">Regulation of small RNA accumulation in the maize shoot apex.</a> PLoS Genet. 5, e1000320-e1000320 (2009).</li></ol>

没有利益冲突的声明。

这里列出的原位杂交方法允许任何感兴趣的基因的时空表达模式的直接可视化的伟大细胞高分辨率，高灵敏度和特异性。该协议不允许之间的基因表达水平的定量比较。然而，该方法的高灵敏度和分辨率，可以揭示一个组织8，18，这是不与其他方法分析基因表达的最可能的基因表达梯度。 该协议包括许多步骤，它可以使故障排除问题。该协议的最重要的步骤是固定的组织和探头的选择和标签。低渗透组织和辛掺入低探头将产生非常微弱的信号，可能很难检测到上述背景。为每个感兴趣的新基因，它是明智的尝试从diffe派生的几个不同的探头租金地区的成绩单。有时候，可能需要两个或三个探测器，针对不同的小区域的基因结合，以获得一个强大的和特定的杂交信号。此外，杂交条件，如温度和杂交缓冲液组成，，可以优化每个基因或组织，以降低或增加杂交紧缩。蛋白酶处理步骤是一个变量，可以改变，以适应不同植物物种或成绩单非常低丰度的检测协议。列入正反两方面的控制探头将有助于查明问题的协议。 石蜡包埋植物组织切片，但稍作修改， 可以在原位杂交用于整个安装过程优化。虽然广泛用于原位杂交动物研究19日 ，整装将升imited到植物组织中，可以很容易地渗透，如胚胎，根或年轻的分生组织20,21。该协议还可以很容易地修改，同时检测两种截然不同的mRNA或本地化一起蛋白 15,22,23的成绩单。最后，它有可能延长这种方法的应用，小分子RNA在植物中的表达模式的可视化。 miRNA表达模式已经确定使用在玉米和拟南芥8,14本议定书。最近， 在体外转录探头已经取代市售高辛标记的锁核酸（LNA），寡核苷酸探针，提高信号灵敏度18，24。

<table border="1"><tbody><tr><th>产品</th><th>公司</th><th>目录编号</th><th>备注</th></tr><tr><td> Cytoseal 604盎司</td><td>费舍尔</td><td> 23-244-256 </td><td>甲苯基于8310-4 </td></tr><tr><td> Histoclear </td><td>费舍尔</td><td> 50-899-90147 </td><td></td></tr><tr><td>组织路径Paraplast加</td><td>费舍尔</td><td> 23-021-400 </td><td></td></tr><tr><td> N +尼龙膜Hybond - N + </td><td> GE医疗集团</td><td> RPN203B </td><td></td></tr><tr><td>核糖核酸酶进行; 40 U /μL </td><td> Invitrogen公司</td><td> 10777019 </td><td></td></tr><tr><td> RQ1无RNase DNA酶; 1 U /μL </td><td> Promega公司</td><td> M6101 </td><td></td></tr><tr><td> DIG标记的RNA控制</td><td>罗氏公司</td><td> 11585746910 </td><td></td></tr><tr><td>辛RNA的标签混合</td><td>罗氏公司</td><td> 11277073910 </td><td></td></tr><tr><td> SP6 RNA聚合酶千ü </td><td>罗氏公司</td><td> 10810274001 </td><tD&gt; </td></tr><tr><td> T3 RNA聚合酶千ü </td><td>罗氏公司</td><td> 11031163001 </td><td></td></tr><tr><td> T7 RNA聚合酶千ü </td><td>罗氏公司</td><td> 10881767001 </td><td></td></tr><tr><td>核糖核酸酶- A </td><td>罗氏公司</td><td> 109142 </td><td>溶于甘油50％三100MM pH8.0 </td></tr><tr><td>阻断试剂</td><td>罗氏公司</td><td> 1 096 176 </td><td>加热到70 ° C的TBS缓冲液</td></tr><tr><td>反Digoxygenin - AP Fab片段，从羊150 U </td><td>罗氏公司</td><td> 1 093 274 </td><td></td></tr><tr><td> NBT / BCIP显原液</td><td>罗氏公司</td><td> 1 681 451 </td><td></td></tr><tr><td>迷你快速旋转的RNA列</td><td>罗氏公司</td><td> 11814427001 </td><td></td></tr><tr><td>的tRNA 从 E 大肠杆菌 </td><td>罗氏公司</td><td> 109541 </td><td></td></tr><tr><td> TRITON ® X - 100 </td><td>西格玛</td><td> T8787 </td><td></td></tr><tr><td>吐温20</td><td>西格玛</td><td> P9416 </td><td></td></tr><tr><td>去离子甲酰胺</td><td>西格玛</td><td> F9037 </td><td></td></tr><tr><td> 灰色链霉菌蛋白酶类型第十四</td><td>西格玛</td><td> P5147 </td><td> milliQ水和预消化4小时稀释在37 ° C </td></tr><tr><td>三乙醇胺</td><td>西格玛</td><td> 90279 </td><td>稀释水，带来用HCl pH8.0 </td></tr><tr><td>醋酐</td><td>西格玛</td><td> A6404 </td><td></td></tr><tr><td> 明串珠菌属硫酸葡聚糖钠盐。 </td><td>西格玛</td><td> D8906 - 5G </td><td>硫酸葡聚糖应加热至80 ° C至解散。 </td></tr><tr><td>登哈特的解决方案50X浓缩</td><td>西格玛</td><td> D2532 </td><td></td></tr><tr><td>牛血清白蛋白 - ≥98％ </td><td>西格玛</td><td> A7906 </td><td></td></tr><tr><td>曙红Y钠盐</td><td>西格玛</td><td> Ë4382 </td><td>在96-100％的乙醇溶解，直至饱和</td></tr><tr><td>多聚甲醛</td><td>西格玛</td><td> P6148 </td><td></td></tr><tr><td>乙醇绝对200的证明</td><td></td><td></td><td></td></tr><tr><td>酚/氯仿</td><td></td><td></td><td></td></tr><tr><td>基地模具</td><td>电子Microsocpy科学</td><td> 62352-15 </td><td></td></tr><tr><td>嵌入戒指</td><td>费舍尔</td><td> 22-038-197 </td><td></td></tr><tr><td> 20毫升一次性闪烁瓶，与帽</td><td> VWR </td><td> 66020-326 </td><td></td></tr><tr><td>探测器上加幻灯片</td><td>费舍尔</td><td> 22-230-900 </td><td></td></tr><tr><td>微盖玻片24x50毫米</td><td> VWR </td><td> 48404-452 </td><td></td></tr><tr><td>滤纸纸3毫米</td><td> VWR </td><td> 89022-360 </td><td></td></tr><tr><td>三玻璃菜和一个STAInless钢滑动架</td><td>电子Microsocpy科学</td><td> 71420-25 </td><td>两片玻璃抛出histoclear治疗的必要，最后一个是醋酸酐处理</td></tr><tr><td> 18个干净的塑料容器</td><td>电子Microsocpy科学</td><td> 71402 </td><td></td></tr><tr><td> 2个或3个大平面密封的盖子的塑料盒</td><td></td><td></td><td>需要的杂交和抗体步骤</td></tr><tr><td>精细刷子</td><td></td><td></td><td>帮助移交蜡色带</td></tr><tr><td>切片机</td><td></td><td></td><td>徕卡</td></tr><tr><td>自清洁的干式真空系统</td><td>韦尔奇</td><td> 2025 </td><td></td></tr><tr><td>滑入温暖</td><td>费舍尔</td><td></td><td></td></tr><tr><td>加热块</td><td></td><td></td><td>需要在60 ° C和85 ° C</td></tr><tr><td>在58-60孵化° C </td><td>费舍尔</td><td></td><td>对于与熔融Paraplast的步骤</td></tr><tr><td> 55 ° C和37 ° C的烤箱或水浴</td><td>费舍尔</td><td></td><td>需要两个由于温度之间的快速变化</td></tr><tr><td>离心（4℃ - 20℃） </td><td></td><td></td><td></td></tr><tr><td>通风柜</td><td></td><td></td><td></td></tr></tbody></table>

in situ hybridization for the precise localization of transcripts in plants

随着基因组学研究在过去十年的进步，植物生物学已经看到大量的研究，提出大型基因表达的定量分析。正在使用的芯片和下一代测序方法调查发育，生理应激反应过程，解剖后生和小RNA的途径，并建立大的基因调控网络1-3。虽然这些技术方便的大型基因组的同时分析，他们通常会提供一个非常有限的时空分辨率，基因表达的变化。这种限制可以通过使用分析方法结合lasermicrodissection或荧光激活细胞分选4-7部分克服。然而，要充分认识的一个基因的生物作用，在细胞决议，其表达的时空格局知识是必不可少的。特别是，当研究发展或环境STI影响木里和突变体的一个基因的表达模式的详细分析变得至关重要。例如，在微妙的关键调控基因的表达水平的定量差异可能导致在特定的细胞类型中表达的损失或收益时，戏剧性的表型。 有几种方法经常用于基因表达模式的详细检查。其一是通过转基因记者线的分析。 ，但是，这种分析可以成为耗时分析多个基因或植物顽抗转型工作。此外，一个独立的验证，以确保转基因表达模式模拟的内源性基因，通常是必需的。免疫组化蛋白定位或mRNA原位杂交中，目前基因表达的细胞和组织内的直接可视化的比较快的替代品。后者具有明显的优势，它可以很容易ü任何感兴趣的基因原位杂交。SED允许一个标记的反义RNA杂交细胞与靶mRNA的检测探头在体外感兴趣的基因的转录获得。 在这里，我们为协议大纲在原位定位基因在植物中的表达，是高度的敏感性和具体。它是使用多聚甲醛固定，石蜡包埋切片，这给组织学的优秀保存，地高辛标记探针免疫检测和碱性磷酸酶比色法反应的可视化进行了优化。该协议已成功地应用于广泛的植物物种组织，可用于分析mRNA的以及小分子RNA 8-14表达。

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In Situ Hybridization for the Precise Localization of Transcripts in Plants

“<em&gt;原位</em&gt;这里描述的杂交协议允许的mRNA和小RNA在细胞水平的高灵敏度和特异性表达的直接本地化。石蜡包埋植物组织切片进行了优化的过程，是适用于广泛的植物和组织，并可以在十天内完成。

原位杂交技术在植物中的精确定位成绩单

With the advances in genomics research of the past decade, plant biology has seen numerous studies presenting large-scale quantitative analyses of gene ...

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In Situ Hybridization for the Precise Localization of Transcripts in Plants

Double Whole Mount in situ Hybridization of Early Chick Embryos

双整装原位杂交技术在早期鸡胚

The chick embryo is a valuable tool in the study of early embryonic development. Its transparency, accessibility and ease of manipulation, make it an ...

科研

生物学

Fluorescence in situ hybridization (FISH) Protocol in Human Sperm

Fluorescence in situ hybridization FISH Protocol in Human Sperm

荧光原位杂交（FISH）的议定书“在人类精子

Aneuploidies are the most frequent chromosomal abnormalities in humans. Most of these abnormalities result from meiotic errors during the gametogenic ...

Radioactive in situ Hybridization for Detecting Diverse Gene Expression Patterns in Tissue

Radioactive in situ Hybridization for Detecting Diverse Gene Expression Patterns in Tissue

放射性的原位杂交检测组织不同的基因表达模式

Knowing the timing, level, cellular localization, and cell type that a gene is expressed in contributes to our understanding of the function of the gene. ...

Detection of Viral RNA by Fluorescence in situ Hybridization (FISH)

Detection of Viral RNA by Fluorescence in situ Hybridization FISH

荧光检测病毒RNA<em&gt;原位</em&gt;杂交（FISH）

Viruses  that infect cells elicit specific changes to normal cell functions which serve  to divert energy and resources for viral replication. Many ...

Analysis of Single-cell Gene Transcription by RNA Fluorescent In Situ Hybridization (FISH)

Analysis of Single-cell Gene Transcription by RNA Fluorescent In Situ Hybridization FISH

单细胞基因转录的RNA荧光分析<em在原位</em&gt;杂交（FISH）

Adhesion of Plasmodium falciparum infected erythrocytes (IE) to human endothelial receptors during malaria infections is mediated by expression of PfEMP1 ...

Chromosome Replicating Timing Combined with Fluorescent In situ Hybridization

Chromosome Replicating Timing Combined with Fluorescent In situ Hybridization

结合荧光染色体复制时序<em在原位</em&gt;杂交

Mammalian DNA replication initiates at multiple sites along chromosomes at different times during S phase, following a temporal replication program. The ...

Whole Mount RNA Fluorescent in situ Hybridization of Drosophila Embryos

Whole Mount RNA Fluorescent in situ Hybridization of Drosophila Embryos

全山RNA荧光<em在原位</em&gt;杂交<em&gt;果蝇</em&gt;胚胎

Assessing  the expression pattern of a gene, as well as the subcellular localization  properties of its transcribed RNA, are key features for ...

Visualization and Analysis of mRNA Molecules Using Fluorescence In Situ Hybridization in Saccharomyces cerevisiae

Visualization and Analysis of mRNA Molecules Using Fluorescence In Situ Hybridization in Saccharomyces cerevisiae

可视化和分析的mRNA分子，使用荧光<em&gt;在原位</em&gt;杂交<em&gt;酿酒酵母（Saccharomyces cerevisiae）</em

The Fluorescence in situ Hybridization (FISH) method allows one to detect nucleic acids in the native cellular environment. Here we provide a protocol for ...

MicroRNA In situ Hybridization for Formalin Fixed Kidney Tissues

MicroRNA In situ Hybridization for Formalin Fixed Kidney Tissues

微RNA<em&gt;原位</em&gt;杂交的福尔马林固定的肾组织

In this article we describe a method for colorimetric detection of miRNA in the kidney through in situ hybridization with digoxigenin tagged microRNA ...

Simple Method for Fluorescence DNA In Situ Hybridization to Squashed Chromosomes

Simple Method for Fluorescence DNA In Situ Hybridization to Squashed Chromosomes

对于荧光DNA的简易方法<em&gt;原位</em&gt;杂交来压扁染色体

DNA in situ hybridization (DNA ISH) is a commonly used method for mapping sequences to specific chromosome regions. This approach is particularly ...