Name: a simplified technique for producing an ischemic wound model
Uploaded: 2012-05-02T12:00:00
Description: Watch this Scientific Journal Video about A Simplified Technique for Producing an Ischemic Wound Model at JoVE.com

部分支持这项研究是由NIH资助1RO1DK74566和1RO1AM52984。笔者想感谢她的帮助杰奎琳·麦卡锡女士在手术过程中，在RRC的DRS。剑谱王和Harshini的萨罗吉尼为帮助他们在一些兔子，荣丸为她组织学工作，博士和她的高效液相色谱法测量高能磷酸盐的李明女士的手术和术后护理。

1。解剖学基础兔耳伤口护理的最惠国待遇研究材料，自20世纪初。耳提供三个维管束（中央，颅，尾）;中央束是最大的，而大小不等的船只在头部和尾部束。偶尔，尾动脉可能缺席。1所有这些船只很容易确定背侧后，耳朵被剃（ 图1）。远端皮肤上腹部，不接受从近端血管的血液供应。背耳上的三个血管束有许多分支，穿透软骨提供血液供应，远...

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糖尿病的影响，在美国，2,3 23.6万人，有15-20％的患者发展足部溃疡，在其一生中，4,5在每年的治疗费用超过25亿美元。3,6然而，即使是最好的治疗愈合率只有50％，并实现这往往是暂时的，复发率高达66％的糖尿病患者7。3,6当溃疡发生在糖尿病患者的主要并发症，如感染和截肢的大幅度增加。尽管近一个世纪广泛的研究和发展成千上万的敷料产品，尚没有证明是有...

在手术过程中的监测<ol><li>一个加热垫，以保持正常的温度。 </li><li>经皮血氧监视器。 </li><li>温度显示器。 </li></ol>手术<ol><li> ＃3＃15刀片与刀。 </li><li>两个双钳（对皮肤和其他软组织的另一个）。 </li><li> 2-3对小夹子。 </li><li>对一个或两个微型剪刀（用于组织切割）。 </li><li>一对小剪刀（用于切割缝合）。 </li><li>一个持针器。 </li><li>一些不锈钢拳（伤口）。 </li></ol>

a simplified technique for producing an ischemic wound model

目前糖尿病伤口研究中的一个主要障碍是缺乏缺血性损伤模型，可以安全地用于糖尿病动物。工作在非缺血性伤口的药物可能无法正常工作，在人类糖尿病患者的伤口，因为血管病变是一个主要因素，阻碍了这些伤口的愈合。我们在2007年发表的文章描述了一种微创外科技术创造一只兔子耳缺血性损伤模型。从那时起，我们已进一步简化，易于操作的过程。一只耳朵上，提出了三个小的皮肤切口上的血管蒂，从耳根1-2厘米。中央动脉结扎和切断神经。整个颅捆绑切断结扎，留下完整的只有尾分行。圆周皮下隧道，通过切口，以减少皮下组织，肌肉，神经，和小型船只。另一只耳朵被用来作为非缺血性的控制。腹侧方Ø四个伤口F各耳。这种技术产生配对比较4缺血性伤口和4个非缺血性伤口在一个动物。手术后，缺血耳朵是凉爽和紫绀，呈减少运动和耳动脉中的脉冲缺乏。缺血耳的皮肤温度为1-10°C下比正常耳，这种差异维持一个月以上。在缺血性比对照耳耳耳组织高能磷酸含量较低。伤口愈合时间长于在缺血性耳在非缺血性耳时使用了相同的待遇。该技术目前已超过80只，其中23例糖尿病（糖尿病的时间从2个星期至2年不等）。没有一个单一的兔子已开发的任何手术并发症，如出血，感染，或在皮肤切口破裂。该模型具有许多优点，如小皮中断，缺血时间较长，成功率较高，许多其他车型相比。它长约n是安全地使用在动物抵抗力降低，也可以修改，以满足不同的测试要求。

Watch this Scientific Journal Video about A Simplified Technique for Producing an Ischemic Wound Model at JoVE.com

A Simplified Technique for Producing an Ischemic Wound Model

我们已经开发出一种微创技术，创建除以中央动脉和神经，颅神经血管束兔子缺血耳朵伤口模型。皮下隧道，然后切开皮下组织。此过程会导致皮肤的破坏最小，可以安全地用于糖尿病动物。

一个生产缺血性损伤模型的简化技术

One major obstacle in current diabetic wound research is a lack of an ischemic wound model that can be safely used in diabetic animals. Drugs that work ...

Research

JoVE Journal

Medicine

Gene Transfer for Ischemic Heart Failure in a Preclinical Model

缺血性心力衰竭在临床前模型的转基因

Various emerging technologies are being developed for patients with heart failure. Well-established preclinical evaluations are necessary to determine ...

科研

医学

Murine Model of Wound Healing

伤口愈合的小鼠模型

Wound healing and repair are the most complex biological processes that occur in human life. After injury, multiple biological pathways become activated. ...

Remote Limb Ischemic Preconditioning:  A Neuroprotective Technique in Rodents

远程肢体缺血预适应：在啮齿动物的保护技术

Sublethal ischemia protects tissues against subsequent, more severe ischemia through the upregulation of endogenous mechanisms in the affected tissue. ...

A Mouse Fetal Skin Model of Scarless Wound Repair

对无瘢痕修复鼠标胎儿皮肤模型

Early in utero, but not in postnatal life, cutaneous wounds undergo regeneration and heal without formation of a scar. Scarless fetal wound healing occurs ...

A Piglet Model of Neonatal Hypoxic-Ischemic Encephalopathy

新生儿缺氧缺血性脑病的小猪模型

Birth asphyxia, which causes hypoxic-ischemic encephalopathy (HIE), accounts for 0.66 million deaths worldwide each year, about a quarter of the ...

Demonstration of the Rat Ischemic Skin Wound Model

在大鼠缺血性皮肤创伤模型的演示

The propensity for chronic wounds in humans increases with ageing, disease conditions such as diabetes and impaired cardiovascular function, and ...

Creation and Transplantation of an Adipose-derived Stem Cell (ASC) Sheet in a Diabetic Wound-healing Model

Creation and Transplantation of an Adipose-derived Stem Cell ASC Sheet in a Diabetic Wound-healing Model

创作和移植的糖尿病伤口愈合模型中脂肪源性干细胞 (ASC) 表

Artificial skin has achieved considerable therapeutic results in clinical practice. However, artificial skin treatments for wounds in diabetic patients ...

Murine Left Anterior Descending (LAD) Coronary Artery Ligation: An Improved and Simplified Model for Myocardial Infarction

Murine Left Anterior Descending LAD Coronary Artery Ligation: An Improved and Simplified Model for Myocardial Infarction

鼠左前降支（LAD）冠状动脉结扎：改进和简化模型的心肌梗死

Ischemic heart disease (IHD), or acute coronary syndrome (ACS), is one of the leading causes of death in the United States. IHD is characterized by ...

Corneal Epithelial Abrasion with Ocular Burr As a Model for Cornea Wound Healing

眼毛刺角膜上皮磨损与角膜创面愈合的模型研究

The murine cornea provides an excellent model to study wound healing. The cornea is the outermost layer of the eye, and thus is the first defense to ...

An Intact Pericardium Ischemic Rodent Model

完整的心包缺血性啮齿动物模型

This protocol has shown that the pericardium and its contents play an essential anti-fibrotic role in the ischemic rodent model (coronary ligation to ...