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DOI :
10.3791/3362-v
January 30th, 2012
Chapters
1:36
Staining Nematodes with DiI
0:05
Title
4:24
Mounting DiI-stained Nematodes
5:40
DiI Staining of C. elegans Cuticle
7:52
Conclusion
我们提出了一个可视化生活角质层的方法 C。线虫使用红色荧光亲脂性染料DII(1,1' - 双十八烷基- 3,3,3',3' - tetramethylindocarbocyanine高氯酸盐),这是常用的 C。线虫可视化环境接触的神经元。这种优化的协议,alae和环形表皮结构是由DII染色和使用复合显微镜观察。
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