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DOI :
10.3791/3370-v
•
10:32 min
January 16th, 2012
Chapters
0:05
Title
2:05
Cloning of the Target Gene/ABDz1-tagged Fusion Construct
4:02
Protein Expression
4:49
Orthogonal Affinity Purification
8:04
Results: SDS-PAGE Analysis of Purified Protein Products
9:45
Conclusion
小説や高効率二段アフィニティークロマトグラフィーのプロトコルが開発され、詳細に説明されている。方法は2つの固有の親和性を持つ小型の精製タグに基づいており、異なるプロパティを持つ標的タンパク質の広い範囲に適用可能です。
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