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DOI :
10.3791/3370-v
•
10:32 min
January 16th, 2012
Chapters
0:05
Title
2:05
Cloning of the Target Gene/ABDz1-tagged Fusion Construct
4:02
Protein Expression
4:49
Orthogonal Affinity Purification
8:04
Results: SDS-PAGE Analysis of Purified Protein Products
9:45
Conclusion
소설과 고효율 두 단계 친화도 크로마 토그래피 프로토콜이 개발되었고 자세하게 설명되어 있습니다. 방법은 두 개의 본질적 동질성과 작은 정화 태그를 기반으로하고 다른 특성을 가진 대상 단백질의 다양한 적용됩니다.
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