Name: selective viral transduction of adult-born olfactory neurons for chronic in vivo optogenetic stimulation
Uploaded: 2011-12-28T12:00:00
Description: Watch this Scientific Journal Video about Selective Viral Transduction of Adult-born Olfactory Neurons for Chronic in vivo Optogenetic Stimulation at JoVE.com

Dette arbejde blev støttet af livsforsikringsselskabet &quot;Novalis-Taitbout&quot;, Ecole des Neurovidenskaber de Paris (ENP), Agence Nationale de la Recherche &quot;ANR-09-NEUR-004&quot; inden for rammerne af &quot;ERA-NET NEURON&quot; af 7. rammeprogram, som Europa-Kommissionen, &quot;Foundation pour la Recherche Medicale&quot;, den Letten Foundation og Pasteur Foundation.

1. Stereotaxic injektioner <ol><li> Den virus, som benyttes i disse forsøg er en replikering-mangel lentiviral vektor baseret på hiv-virus til at udtrykke Channelrhodopsine2-YFP (gul fluorescerende protein) Fusion konstruere drevet af en synapsin promotor 6. Den plasmid blev gavmildt leveret af K. Deisseroth &#39;s Research Group ( <a href="http://www.stanford.edu/group/dlab/optogenetics/index.html">http://www.stanford.edu/group/dlab/optogenetics/index.html</a> ). Lentiviruses kan fås fra kommercielle ki...

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Optogenetic værktøjer har for nylig øget vores kontrol over selektiv neuronal befolkninger, herunder voksne født neuroner i de olfaktoriske system. Her beskriver vi, hvordan du udfører en stereotaxic injektion af en lentiviral vektor udtrykke ChR2-YFP i en bestemt population af voksne født neuroner. Ved nøje at overvåge den periode efter injektion, er vi i stand til at analysere og manipulere aktiviteten af ​​en kohorte af voksen-født neuroner med en relativ homogen alder. På g...

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selective viral transduction of adult-born olfactory neurons for chronic in vivo optogenetic stimulation

Lokale interneuroner løbende regenereres i lugtekolben af voksne gnavere 1-3. I denne proces, der kaldes voksen neurogenese, neurale stamceller i væggene af lateral ventrikel give anledning til neuroblasts at migrere til flere millimeter langs rostralt vandrende stream (RMS) til at nå og indarbejde de olfaktoriske pære. For at studere de forskellige trin og virkningen af ​​voksen-fødte neuron integration i allerede eksisterende olfaktoriske kredsløb, er det nødvendigt at selektivt mærke og manipulere aktiviteten af ​​dette specifikke population af neuroner. Den seneste udvikling af optogenetic teknologier giver mulighed for at bruge lys til at netop aktivere denne særlige gruppe af neuroner, uden at påvirke omkringliggende neuroner 4,5. Her præsenterer vi en række procedurer for at viralt udtrykke Channelrhodopsin2 (ChR2)-YFP i en tidsmæssigt begrænset kohorte af neuroblasts i RMS, inden de når frem til lugtekolben og blive voksen-fødte neurons. Derudover viser vi, hvordan man implantat og kalibrere en miniature LED for kronisk in vivo stimulering af ChR2-udtrykkende neuroner.

Watch this Scientific Journal Video about Selective Viral Transduction of Adult-born Olfactory Neurons for Chronic in vivo Optogenetic Stimulation at JoVE.com

Selective Viral Transduction of Adult-born Olfactory Neurons for Chronic in vivo Optogenetic Stimulation

Voksen-født neuroner i de olfaktoriske pære kan optogenetically styres med Channelrhodopsin2-udtrykkende lentiviral injektion i rostralt vandrende åen og kroniske photostimulation med en implanteret miniature LED.

Selektiv Viral transduktion af Voksen-fødte olfaktoriske neuroner for Kronisk In vivo Optogenetic Stimulation

Local interneurons are continuously regenerated in the olfactory bulb of adult rodents1-3. In this process, called adult neurogenesis, neural stem cells ...

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JoVE Journal

Neuroscience

Selective Viral Transduction of Adult-born Olfactory Neurons for Chronic in vivo Optogenetic Stimulation

Studying the Integration of Adult-born Neurons

Neurogenesis occurs in adult mammalian brains in the sub-ventricular zone (SVZ) of the lateral ventricle and in the sub-granular zone (SGZ) of the ...

Lentivirus-mediated Genetic Manipulation and Visualization of Olfactory Sensory Neurons in vivo

Lentivirus-mediated Genetic Manipulation and Visualization of Olfactory Sensory Neurons in vivo

Development of a precise olfactory circuit relies on accurate projection of olfactory sensory neuron (OSN) axons to their synaptic 
targets in the ...

Flash Photolysis of Caged Compounds in the Cilia of Olfactory Sensory  Neurons

Flash fotolyse af Caged forbindelser i Cilia af olfaktoriske sensoriske neuroner

Photolysis of caged compounds allows the production of rapid and localized increases in the concentration of various physiologically active compounds1. ...

Fiber-optic Implantation for Chronic Optogenetic Stimulation of Brain Tissue

Fiberoptisk implantation for kronisk Optogenetic Stimulation af hjernevæv

Elucidating patterns of neuronal connectivity has been a challenge for both clinical and basic neuroscience. Electrophysiology has been the gold standard ...

Optogenetic Stimulation of Escape Behavior in Drosophila melanogaster

Optogenetic Stimulation of Escape Behavior in Drosophila melanogaster

Optogenetic Stimulering af flugt Opførsel i<em&gt; Drosophila melanogaster</em

A  growing number of genetically encoded tools are becoming available that allow non-invasive  manipulation of the neural activity of specific neurons in ...

A Method for High Fidelity Optogenetic Control of Individual Pyramidal Neurons In vivo

A Method for High Fidelity Optogenetic Control of Individual Pyramidal Neurons In vivo

Metode til High Fidelity optogenetic Kontrol af Individuelle pyramideneuroner<em&gt; In vivo</em

Optogenetic methods  have emerged as a powerful tool for elucidating neural circuit activity  underlying a diverse set of behaviors across a broad range ...

In vivo Optogenetic Stimulation of the Rodent Central Nervous System

In vivo Optogenetic Stimulation of the Rodent Central Nervous System

In vivo optogenetic Stimulering af Rodent centralnervesystemet

The ability to probe defined neural circuits in awake, freely-moving animals with cell-type specificity, spatial precision, and high temporal resolution ...

Intracerebroventricular Viral Injection of the Neonatal Mouse Brain for Persistent and Widespread Neuronal Transduction

Intracerebroventrikulær Viral Injektion af Neonatal Mouse Hjerne for Vedvarende og Udbredt Neuronal transduktion

With the pace of scientific advancement accelerating rapidly, new methods are needed for experimental neuroscience to quickly and easily manipulate gene ...

Optogenetic Stimulation of the Auditory Nerve

Optogenetic Stimulering af hørenerven

Direct electrical stimulation of spiral ganglion neurons (SGNs) by cochlear implants (CIs) enables open speech comprehension in the majority of implanted ...

Ex Vivo Optogenetic Dissection of Fear Circuits in Brain Slices

Ex Vivo Optogenetic Dissection of Fear Circuits in Brain Slices

Ex Vivo optogenetic Dissektion af Frygt Kredsløb i Brain Skiver

Optogenetic approaches are now widely used to study the function of neural populations and circuits by combining targeted expression of light-activated ...