Name: selective viral transduction of adult-born olfactory neurons for chronic in vivo optogenetic stimulation
Uploaded: 2011-12-28T12:00:00
Description: Watch this Scientific Journal Video about Selective Viral Transduction of Adult-born Olfactory Neurons for Chronic in vivo Optogenetic Stimulation at JoVE.com

Dit werk werd ondersteund door het leven verzekeringsmaatschappij &quot;Novalis-Taitbout&quot;, Ecole des Neurowetenschappen de Paris (ENB), het Agence Nationale de la Recherche &quot;ANR-09-neur-004&quot; in het kader van &quot;ERA-NET NEURON&quot; van KP7 programma door de Europese Commissie, &quot;Stichting pour la Recherche Medicale&quot;, de Letten Foundation en de Stichting Pasteur.

1. Stereotaxische injecties <ol><li> Het virus gebruikt in deze experimenten is een replicatie-deficiënte lentivirale vector op basis van het HIV-virus te Channelrhodopsine2-YFP (geel fluorescerend eiwit) te uiten fusieconstruct aangedreven door een synapsin promotor 6. Het plasmide werd royaal door K. Deisseroth &#39;s onderzoeksgroep ( <a href="http://www.stanford.edu/group/dlab/optogenetics/index.html">http://www.stanford.edu/group/dlab/optogenetics/index.html</a> ). Lentivirussen kan worden verkregen u...

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Optogenetic tools hebben onlangs gestegen onze controle over de selectieve neuronale populaties, met inbegrip van volwassen geboren neuronen in de olfactorische systeem. Hier beschrijven we hoe u een stereotaxische injectie van een lentivirale vector die ChR2-YFP in een specifieke populatie van volwassen geboren neuronen uit te voeren. Door zorgvuldig toezicht op de periode na de injectie, zijn we in staat om te analyseren en manipuleren van de activiteit van een cohort van volwassen geboren neuronen met een relatief ho...

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selective viral transduction of adult-born olfactory neurons for chronic in vivo optogenetic stimulation

Lokale interneuronen worden continu geregenereerd in de bulbus olfactorius van volwassen knaagdieren 1-3. In dit proces, de zogenaamde volwassen neurogenese, neurale stamcellen in de muren van de laterale ventrikel aanleiding geven tot neuroblasts die migreren van enkele millimeters langs de rostrale trekkende stroom (RMS) te bereiken en op te nemen in de bulbus olfactorius. De studie van de verschillende stappen en de impact van volwassen geboren neuron integratie in de reeds bestaande olfactorische circuits, is het noodzakelijk om selectief label en de activiteit van deze specifieke populatie van neuronen te manipuleren. De recente ontwikkeling van optogenetic technologieën biedt de mogelijkheid om licht te gebruiken om deze specifieke cohort van neuronen precies te activeren zonder dat dit invloed omliggende neuronen 4,5. Hier presenteren wij een reeks van procedures om viraal te uiten Channelrhodopsin2 (ChR2)-YFP in een tijdelijk beperkte cohort van neuroblasts in de RMS voordat ze de bulbus olfactorius en worden volwassen geboren neurons. Daarnaast hebben we laten zien hoe implantaat en kalibreren een miniatuur LED voor chronische in vivo stimulatie van ChR2-expressie neuronen.

Watch this Scientific Journal Video about Selective Viral Transduction of Adult-born Olfactory Neurons for Chronic in vivo Optogenetic Stimulation at JoVE.com

Selective Viral Transduction of Adult-born Olfactory Neurons for Chronic in vivo Optogenetic Stimulation

Volwassen geboren neuronen van de bulbus olfactorius kan optogenetically worden gecontroleerd met behulp van Channelrhodopsin2 expressie lentivirale injectie in de rostrale migratie stroom en chronische fotostimulering met een geïmplanteerd miniatuur LED.

Selectieve Viral Transductie van Adult geboren Olfactorische Neuronen voor chronische In vivo Optogenetic Stimulatie

Local interneurons are continuously regenerated in the olfactory bulb of adult rodents1-3. In this process, called adult neurogenesis, neural stem cells ...

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Neuroscience

Selective Viral Transduction of Adult-born Olfactory Neurons for Chronic in vivo Optogenetic Stimulation

Studying the Integration of Adult-born Neurons

Het bestuderen van de integratie van Adult geboren Neuronen

Neurogenesis occurs in adult mammalian brains in the sub-ventricular zone (SVZ) of the lateral ventricle and in the sub-granular zone (SGZ) of the ...

Lentivirus-mediated Genetic Manipulation and Visualization of Olfactory Sensory Neurons in vivo

Lentivirus-mediated Genetic Manipulation and Visualization of Olfactory Sensory Neurons in vivo

Lentivirus-gemedieerde genetische manipulatie en visualisatie van olfactorische sensorische neuronen In vivo</em

Development of a precise olfactory circuit relies on accurate projection of olfactory sensory neuron (OSN) axons to their synaptic 
targets in the ...

Flash Photolysis of Caged Compounds in the Cilia of Olfactory Sensory  Neurons

Flash fotolyse van Gekooide verbindingen in de Cilia van olfactorische sensorische neuronen

Photolysis of caged compounds allows the production of rapid and localized increases in the concentration of various physiologically active compounds1. ...

Fiber-optic Implantation for Chronic Optogenetic Stimulation of Brain Tissue

Glasvezel-implantatie voor chronische Optogenetic Stimulatie van hersenweefsel

Elucidating patterns of neuronal connectivity has been a challenge for both clinical and basic neuroscience. Electrophysiology has been the gold standard ...

Optogenetic Stimulation of Escape Behavior in Drosophila melanogaster

Optogenetic Stimulation of Escape Behavior in Drosophila melanogaster

Optogenetic Stimulatie van Escape Gedrag in<em&gt; Drosophila melanogaster</em

A  growing number of genetically encoded tools are becoming available that allow non-invasive  manipulation of the neural activity of specific neurons in ...

A Method for High Fidelity Optogenetic Control of Individual Pyramidal Neurons In vivo

A Method for High Fidelity Optogenetic Control of Individual Pyramidal Neurons In vivo

Een methode voor High Fidelity optogenetic controle van individuele piramidale neuronen<em&gt; In vivo</em

Optogenetic methods  have emerged as a powerful tool for elucidating neural circuit activity  underlying a diverse set of behaviors across a broad range ...

In vivo Optogenetic Stimulation of the Rodent Central Nervous System

In vivo Optogenetic Stimulation of the Rodent Central Nervous System

In vivo optogenetische Stimulatie van de knaagdieren centrale zenuwstelsel

The ability to probe defined neural circuits in awake, freely-moving animals with cell-type specificity, spatial precision, and high temporal resolution ...

Intracerebroventricular Viral Injection of the Neonatal Mouse Brain for Persistent and Widespread Neuronal Transduction

Intracerebroventriculaire Viral Injectie van de Neonatale Mouse Brain voor persistent en wijdverbreide Neuronale Transduction

With the pace of scientific advancement accelerating rapidly, new methods are needed for experimental neuroscience to quickly and easily manipulate gene ...

Optogenetic Stimulation of the Auditory Nerve

Optogenetische stimulatie van de gehoorzenuw

Direct electrical stimulation of spiral ganglion neurons (SGNs) by cochlear implants (CIs) enables open speech comprehension in the majority of implanted ...

Ex Vivo Optogenetic Dissection of Fear Circuits in Brain Slices

Ex Vivo Optogenetic Dissection of Fear Circuits in Brain Slices

Ex Vivo optogenetische Dissection of Fear Circuits in Brain Slices

Optogenetic approaches are now widely used to study the function of neural populations and circuits by combining targeted expression of light-activated ...