Name: selective viral transduction of adult-born olfactory neurons for chronic in vivo optogenetic stimulation
Uploaded: 2011-12-28T12:00:00
Description: Watch this Scientific Journal Video about Selective Viral Transduction of Adult-born Olfactory Neurons for Chronic in vivo Optogenetic Stimulation at JoVE.com

Detta arbete stöddes av livbolaget &quot;Novalis-Taitbout&quot;, Ecole des Neurovetenskap de Paris (ENP), Agence Nationale de la Recherche &quot;ANR-09-neur-004&quot; inom ramen för &quot;ERA-NET NEURON&quot; av FP7-program från Europeiska kommissionen, &quot;Stiftelsen pour la Recherche médicale&quot;, den Letten Foundation och Pasteur stiftelsen.

1. Stereotaxic injektioner <ol><li> Det virus som används i dessa experiment är en replikering-brist Lentiviral vektor baserad på hiv-viruset att uttrycka Channelrhodopsine2-YFP (gul fluorescerande protein) Fusion konstruera drivs av en synapsin promotor 6. Den plasmid var generöst tillhandahålls av K. Deisseroth forskargrupp ( <a href="http://www.stanford.edu/group/dlab/optogenetics/index.html">http://www.stanford.edu/group/dlab/optogenetics/index.html</a> ). Lentiviruses kan erhållas från kommersie...

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Optogenetic verktyg har nyligen ökat vår kontroll över selektiv neuronal befolkningar, inklusive vuxna född nervceller i luktsinne. Här beskriver vi hur du utför en stereotaxic injektion av en Lentiviral vektor som uttrycker ChR2-YFP i en viss population av vuxna födda nervceller. Genom att noggrant övervaka perioden efter injektion, kan vi analysera och manipulera aktiviteten hos en kohort av vuxna födda nervceller med en relativt homogen ålder. Grund av deras låga spridning spri...

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selective viral transduction of adult-born olfactory neurons for chronic in vivo optogenetic stimulation

Lokala interneuronen ständigt återskapas i luktloben av vuxna gnagare 1-3. I denna process, som kallas vuxen neurogenes, neurala stamceller i väggarna i den laterala ventrikeln ger upphov till neuroblaster att migrera flera millimeter längs rostralt vandrande ström (RMS) för att nå och införliva luktbulben. För att studera de olika stegen och effekterna av vuxna födda neuron integration i redan existerande lukt kretsar, är det nödvändigt att selektivt märka och manipulera aktiviteten hos denna specifika populationen av nervceller. Den senaste tidens utveckling av optogenetic teknik ger möjlighet att använda ljus för att exakt aktivera denna specifika kohort av nervceller utan att påverka omgivande nervceller 4,5. Här presenterar vi en rad förfaranden för att viralt uttrycka Channelrhodopsin2 (ChR2)-YFP i en tidsmässigt begränsad kohort av neuroblaster i RMS innan de når luktbulben och bli vuxna födda neurons. Dessutom visar vi hur implantatet och kalibrera en miniatyr LED för kronisk in vivo stimulering av ChR2-uttryckande nervceller.

Watch this Scientific Journal Video about Selective Viral Transduction of Adult-born Olfactory Neurons for Chronic in vivo Optogenetic Stimulation at JoVE.com

Selective Viral Transduction of Adult-born Olfactory Neurons for Chronic in vivo Optogenetic Stimulation

Vuxna födda nervceller i luktloben kan optogenetically kontrolleras med Channelrhodopsin2-uttryckande Lentiviral injektion i rostralt flyttande strömmen och kronisk photostimulation med en inopererad miniatyr lysdiod.

Selektiv Viral nervsystemet av vuxna födda Olfactory Neuroner för kronisk In vivo Optogenetic Stimulering

Local interneurons are continuously regenerated in the olfactory bulb of adult rodents1-3. In this process, called adult neurogenesis, neural stem cells ...

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Neuroscience

Selective Viral Transduction of Adult-born Olfactory Neurons for Chronic in vivo Optogenetic Stimulation

Studying the Integration of Adult-born Neurons

Att studera integration av vuxna födda Neuroner

Neurogenesis occurs in adult mammalian brains in the sub-ventricular zone (SVZ) of the lateral ventricle and in the sub-granular zone (SGZ) of the ...

Lentivirus-mediated Genetic Manipulation and Visualization of Olfactory Sensory Neurons in vivo

Lentivirus-mediated Genetic Manipulation and Visualization of Olfactory Sensory Neurons in vivo

Lentivirus-medierad genetisk manipulation och visualisering av Olfactory sensoriska neuroner In vivo</em

Development of a precise olfactory circuit relies on accurate projection of olfactory sensory neuron (OSN) axons to their synaptic 
targets in the ...

Flash Photolysis of Caged Compounds in the Cilia of Olfactory Sensory  Neurons

Flash Fotolys av Caged föreningar i Cilia av Olfactory sensoriska neuroner

Photolysis of caged compounds allows the production of rapid and localized increases in the concentration of various physiologically active compounds1. ...

Fiber-optic Implantation for Chronic Optogenetic Stimulation of Brain Tissue

Fiberoptisk Implantation för kronisk optogenetic Stimulering av hjärnvävnad

Elucidating patterns of neuronal connectivity has been a challenge for both clinical and basic neuroscience. Electrophysiology has been the gold standard ...

Optogenetic Stimulation of Escape Behavior in Drosophila melanogaster

Optogenetic Stimulation of Escape Behavior in Drosophila melanogaster

Optogenetic Stimulering av Escape Beteende i<em&gt; Drosophila melanogaster</em

A  growing number of genetically encoded tools are becoming available that allow non-invasive  manipulation of the neural activity of specific neurons in ...

A Method for High Fidelity Optogenetic Control of Individual Pyramidal Neurons In vivo

A Method for High Fidelity Optogenetic Control of Individual Pyramidal Neurons In vivo

En metod för High Fidelity Optogenetic kontroll av enskilda pyramidala nervceller<em&gt; In vivo</em

Optogenetic methods  have emerged as a powerful tool for elucidating neural circuit activity  underlying a diverse set of behaviors across a broad range ...

In vivo Optogenetic Stimulation of the Rodent Central Nervous System

In vivo Optogenetic Stimulation of the Rodent Central Nervous System

In vivo optogenetic Stimulering av gnagare centrala nervsystemet

The ability to probe defined neural circuits in awake, freely-moving animals with cell-type specificity, spatial precision, and high temporal resolution ...

Intracerebroventricular Viral Injection of the Neonatal Mouse Brain for Persistent and Widespread Neuronal Transduction

Intracerebroventrikulär Viral Injektion av neonatal mus hjärnans för långlivade och Utbredd neuronal Transduktion

With the pace of scientific advancement accelerating rapidly, new methods are needed for experimental neuroscience to quickly and easily manipulate gene ...

Optogenetic Stimulation of the Auditory Nerve

Optogenetic Stimulering av hörselnerven

Direct electrical stimulation of spiral ganglion neurons (SGNs) by cochlear implants (CIs) enables open speech comprehension in the majority of implanted ...

Ex Vivo Optogenetic Dissection of Fear Circuits in Brain Slices

Ex Vivo Optogenetic Dissection of Fear Circuits in Brain Slices

Ex vivo optogenetic Dissekering av skräck kretsar i hjärnan skivor

Optogenetic approaches are now widely used to study the function of neural populations and circuits by combining targeted expression of light-activated ...