Name: utilization of plasmonic and photonic crystal nanostructures for enhanced micro- and nanoparticle manipulation
Uploaded: 2011-09-27T12:00:00
Description: Watch this Scientific Journal Video about Utilization of Plasmonic and Photonic Crystal Nanostructures for Enhanced Micro- and Nanoparticle Manipulation at JoVE.com

نود أيضا أن نشكر شياو يو مياو ويلسون بن لتطوير أكثر من الأساليب المذكورة داخل. وقد تم تمويل هذا العمل من قبل المؤسسة الوطنية للعلوم (DBI 0454324) والمعهد الوطني للصحة (R21 EB005183) وPHS NRSA T32 GM07270 NIGMS من لجنة الانتخابات.

1. عشوائية صفيف الاتحاد الافريقي nanoparticle التصنيع 8،10،12،14 <ol style=";text-align:right;direction:rtl"><li style=";text-align:right;direction:rtl"> يتم تشكيل مجموعة جسيمات متناهية الصغر من خلال إنشاء الاتحاد الافريقي لأول مرة القالب الذي يتكون من طبقة كثيفة من المجالات اللاتكس كثف عشوائيا مع قطر يعني ?...

<ol>
	<li>Jones, T. B. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=.">.</a> Electromechanics of Particles. , (1995).</li><li>Ashkin, A. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Optical+trapping+and+manipulation+of+neutral+particles+using+lasers.">Optical trapping and manipulation of neutral particles using lasers.</a> Proc. Natl. Acad. Sci. U.S.A. 94, 4853-4853 (1997).</li><li>Neuman, K. C., Chadd, E. H., Liou, G. F., Bergman, K., Block, S. M. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Characterization+of+photodamage+to+Escherichia+coli+in+optical+traps.">Characterization of photodamage to Escherichia coli in optical traps.</a> Biophys. J. 77, 2856-2856 (1999).</li><li>Chiou, P. C., Ohta, A. T., Wu, M. C. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Massively+parallel+manipulation+of+single+cells+and+microparticles+using+optical+images.">Massively parallel manipulation of single cells and microparticles using optical images.</a> Nature. 436, 370-370 (2005).</li><li>Hsu, H. Y., Ohta, A. T., Chiou, P. Y., Jamshidi, A., Nealea, S. L., Wua, M. C. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Phototransistor-based+optoelectronic+tweezers+for+dynamic+cell+manipulation+in+cell+culture+media.">Phototransistor-based optoelectronic tweezers for dynamic cell manipulation in cell culture media.</a> Lab Chip. 10, 165-172 (2010).</li><li>Righini, M., Ghenuche, P. S., Cherukulappurath, V., Myroshnychenko, F. J., Garcia de Abajo, R. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Quidant+Nano-optical+Trapping+of+Rayleigh+Particles+Escherichia+coli+Bacteria+with+Resonant+Optical+Antennas.">Quidant Nano-optical Trapping of Rayleigh Particles Escherichia coli Bacteria with Resonant Optical Antennas.</a> Nano Letters. 9, 3387-3391 (2009).</li><li>Righini, M., Zelenina, A. S., Girard, C., Quidant, R. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Parallel+and+Selective+Trapping+in+a+Patterned+Plasmonic+Landscape.">Parallel and Selective Trapping in a Patterned Plasmonic Landscape.</a> Nature Physics. 3, 477-480 (2007).</li><li>Miao, X., Lin, L. Y. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Large+dielectrophoresis+force+and+torque+induced+by+localized+surface+plasmon+resonance+of+a+cap-shaped+Au+nanoparticle+array.">Large dielectrophoresis force and torque induced by localized surface plasmon resonance of a cap-shaped Au nanoparticle array.</a> Opt. Lett. 32, 295-297 (2007).</li><li>Wilson, B. K. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=.">.</a> Manipulation of Nanoparticles and Biological Samples through Enhanced Optical Forces [dissertation]. , (2009).</li><li>Miao, X. Y., Wilson, B. K., Pun, S. H., Lin, L. Y. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Optical+manipulation+of+micron/submicron+sized+particles+and+biomolecules+through+plasmonics.">Optical manipulation of micron/submicron sized particles and biomolecules through plasmonics.</a> Optics Exp. 16, 13517-13525 (2008).</li><li>Wilson, B. K., Mentele, T., Bachar, S., Knouf, E., Bendoraite, A., Tewari, M., Pun, S. H., Lin, L. Y. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Nanostructure-enhanced+laser+tweezers+for+efficient+trapping+and+alignment+of+particles.">Nanostructure-enhanced laser tweezers for efficient trapping and alignment of particles.</a> Optics. Exp. 18, 16005-16013 (2010).</li><li>Miao, X., Wilson, B. K., Cao, G., Pun, S. H., Lin, L. Y. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Trapping+and+Rotation+of+Nanowires+Assisted+by+Surface+Plasmons.">Trapping and Rotation of Nanowires Assisted by Surface Plasmons.</a> IEEE Journal of Selected Topics in Quantum Electronics. 15, 1515-1520 (2009).</li><li>Miao, X. Y., Lin, L. Y. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Trapping+and+manipulation+of+biological+particles+through+a+plasmonic+platform.">Trapping and manipulation of biological particles through a plasmonic platform.</a> IEEE Journal of Selected Topics in Quantum Electronics. 13, 1655-1662 (2007).</li><li>Miao, X. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=.">.</a> Plasmonics for Micro/Nano Manipulation and Optofluidics [dissertation]. , (2008).</li></ol>

المغزى من هذه الأساليب لأنها محاصرة انخفاض كثافة الضوئية اللازمة لمحاصرة مستمرة من مكان ما على ترتيب مكان للμW 10 3 / 2 ميكرون بناء على أمر من μW 10 / 2 ميكرون. 10،11 والقيود المفروضة على هذه التقنيات هي أن جسيمات متناهية الصغر مجموعة الذهب الخبرات الت...

<table border="1" style=";text-align:right;direction:rtl"><tr><td> اسم المادة </td><td> نوع </td><td> شركة </td><td> رقم كتالوج </td><td> تعليق </td></tr><tr><td> تصوير محوري مجهر </td><td> D1M </td><td> زايس </td><td> D1M </td><td> زايس محوري Imager.D1M </td></tr><tr><td> الهدف المجهر </td><td> 50x/0.55 </td><td> زايس </td><td></td><td> LD EC Epiplan -- NEOFLUAR 50x/0.55 HD مدينة دبي للإنترنت </td></tr><tr><td> زايس مجهر الكاميرا </td><td> AxioCam MRC </td><td> زايس </td><td></td><td></td></tr><tr><td> ليزر الهليوم نيون </td><td> 35 ميغاواط </td><td> البحوث الكهربائية والبصريات </td><td></td><td></td></tr><tr><td> متغير المخفف </td><td> متغير باستمرار ND </td><td> ThorLabs </td><td> NDC - 100C - 4M </td><td> لضبط شدة المجهر </td></tr><tr><td> تصفية مجموعة زايس </td><td> تصفية مجموعة # 17 </td><td> زايس </td><td> 488017-9901-000 </td><td> تصفية مجموعة # 17 </td></tr><tr><td> المجهر الشرائح </td><td> سماكة 0.5 مم </td><td> VWR </td><td></td><td></td></tr><tr><td> 3T3 نواة الخلية الماوس </td><td></td><td colspan="2"> فريد هاتشينسون لبحوث السرطان مركز </td><td> مثل البرد ممكن مخزن </td></tr><tr><td> أكريدين صبغ برتقالي </td><td></td><td colspan="2"> فريد هاتشينسون لبحوث السرطان مركز </td><td></td></tr><tr><td> الأبقار مصل الزلال </td><td> 1-10 حصة في برنامج تلفزيوني </td><td colspan="2"> فريد هاتشينسون لبحوث السرطان مركز </td><td></td></tr><tr><td> 454 نانومتر المجالات اللاتكس البوليسترين </td><td></td><td> Polysciences ، وشركة </td><td></td><td></td></tr><tr><td> carbodiimide هيدروكلوريد (EDC) </td><td> 1 إيثيل - 3 - (3 - dimethylaminopropyl) </td><td> G - العلوم البيولوجية </td><td> BC25 - 1 </td><td></td></tr><tr><td> الذهب (لترسب) </td><td></td><td></td><td></td><td></td></tr><tr><td> تعكس مقضب الحيود حكمت </td><td></td><td> ادموند بصريات </td><td></td><td></td></tr><tr><td> التخزين المؤقت المالحة الفوسفات (PBS) </td><td> Dulbecco والفوسفات مخزنة المالحة (D - PBS) (1X) </td><td> Invitrogen </td><td> 14190-144 </td><td></td></tr></table>

utilization of plasmonic and photonic crystal nanostructures for enhanced micro- and nanoparticle manipulation

A method to manipulate the position and orientation of submicron particles nondestructively would be an incredibly useful tool for basic biological research. Perhaps the most widely used physical force to achieve noninvasive manipulation of small particles has been dielectrophoresis(DEP).1 However, DEP on its own lacks the versatility and precision that are desired when manipulating cells since it is traditionally done with stationary electrodes. Optical tweezers, which utilize a three dimensional electromagnetic field gradient to exert forces on small particles, achieve this desired versatility and precision.2 However, a major drawback of this approach is the high radiation intensity required to achieve the necessary force to trap a particle which can damage biological samples.3 A solution that allows trapping and sorting with lower optical intensities are optoelectronic tweezers (OET) but OET's have limitations with fine manipulation of small particles; being DEP-based technology also puts constraint on the property of the solution.4,5
This video article will describe two methods that decrease the intensity of the radiation needed for optical manipulation of living cells and also describe a method for orientation control. The first method is plasmonic tweezers which use a random gold nanoparticle (AuNP) array as a substrate for the sample as shown in Figure 1. The AuNP array converts the incident photons into localized surface plasmons (LSP) which consist of resonant dipole moments that radiate and generate a patterned radiation field with a large gradient in the cell solution. Initial work on surface plasmon enhanced trapping by Righini et al and our own modeling have shown the fields generated by the plasmonic substrate reduce the initial intensity required by enhancing the gradient field that traps the particle.6,7,8 The plasmonic approach allows for fine orientation control of ellipsoidal particles and cells with low optical intensities because of more efficient optical energy conversion into mechanical energy and a dipole-dependent radiation field. These fields are shown in figure 2 and the low trapping intensities are detailed in figures 4 and 5. The main problems with plasmonic tweezers are that the LSP's generate a considerable amount of heat and the trapping is only two dimensional. This heat generates convective flows and thermophoresis which can be powerful enough to expel submicron particles from the trap.9,10 The second approach that we will describe is utilizing periodic dielectric nanostructures to scatter incident light very efficiently into diffraction modes, as shown in figure 6.11 Ideally, one would make this structure out of a dielectric material to avoid the same heating problems experienced with the plasmonic tweezers but in our approach an aluminum-coated diffraction grating is used as a one-dimensional periodic dielectric nanostructure. Although it is not a semiconductor, it did not experience significant heating and effectively trapped small particles with low trapping intensities, as shown in figure 7. Alignment of particles with the grating substrate conceptually validates the proposition that a 2-D photonic crystal could allow precise rotation of non-spherical micron sized particles.10 The efficiencies of these optical traps are increased due to the enhanced fields produced by the nanostructures described in this paper.

Watch this Scientific Journal Video about Utilization of Plasmonic and Photonic Crystal Nanostructures for Enhanced Micro- and Nanoparticle Manipulation at JoVE.com

Utilization of Plasmonic and Photonic Crystal Nanostructures for Enhanced Micro- and Nanoparticle Manipulation

وترد وملاقط Plasmonic النانو الكريستال الضوئية لإنتاج تحسينات مفيدة في السيطرة والتوجيه من الكفاءة محاصرة بصريا المتناهية الصغر ومتناهية الصغر.

الاستفادة من النانو كريستال Plasmonic والضوئية لتعزيز التلاعب الصغرى ، والجسيمات النانوية

A method to manipulate the position and orientation of submicron particles nondestructively would be an incredibly useful tool for basic biological ...

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تقييم توصيل الجينات البوليمرية الجسيمات النانوية من تحليل تتبع جسيمات متناهية الصغر وعالية الإنتاجية قياس التدفق الخلوي

Non-viral gene delivery using polymeric nanoparticles has emerged as an attractive approach for gene therapy to treat genetic diseases1 and as a ...

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Micro-particle image velocimetry (&mu;PIV) is used to visualize paired images of micro particles seeded in blood flows. The images are cross-correlated to ...

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ألياف النانو البروتين ECM والنانو المهندسة طريق الجمعية بمبادرة السطحية

The extracellular matrix (ECM) in tissues is synthesized and assembled by cells to form a 3D fibrillar, protein network with tightly regulated fiber ...

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استهدفت البلازما غشاء تسليم ومسعور الشحن مغلف في الكريستال السائل الجسيمات النانوية الناقل

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Here, we present a step-by-step preparation of a 3D, biodegradable, foam-like cell scaffold. These scaffolds were prepared by cross-linking star block ...