Name: utilization of plasmonic and photonic crystal nanostructures for enhanced micro- and nanoparticle manipulation
Uploaded: 2011-09-27T12:00:00
Description: Watch this Scientific Journal Video about Utilization of Plasmonic and Photonic Crystal Nanostructures for Enhanced Micro- and Nanoparticle Manipulation at JoVE.com

我们还要感谢小玉苗和Ben威尔逊发展的最内所述的方法。这项工作是由国家科学基金会（DBI 0454324）和国家卫生研究所（R21 EB005183）和ECK NIGMS小灵通NRSA的T32 GM07270。

1。随机的Au纳米粒子阵列的制备 8,10,12,14 <ol><li>首先创建一个模板，是一个随机吸附乳胶球，平均直径454纳米的致密层形成的Au纳米粒子阵列。这是通过首先蒸发20铬纳米粘附层的厚度玻璃盖玻片黄金。 </li><li>聚苯乙烯球暴露镀金基板的混合物，然后自组装单层是1 - 乙基-3 - （3 - dimethylaminopropyl）碳二亚胺盐酸盐（EDC），乳胶球体悬浮液和去离子水。 </li><li>吸附过程是可以持续约一小时?...

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	<li>Jones, T. B. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=.">.</a> Electromechanics of Particles. , (1995).</li><li>Ashkin, A. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Optical+trapping+and+manipulation+of+neutral+particles+using+lasers.">Optical trapping and manipulation of neutral particles using lasers.</a> Proc. Natl. Acad. Sci. U.S.A. 94, 4853-4853 (1997).</li><li>Neuman, K. C., Chadd, E. H., Liou, G. F., Bergman, K., Block, S. M. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Characterization+of+photodamage+to+Escherichia+coli+in+optical+traps.">Characterization of photodamage to Escherichia coli in optical traps.</a> Biophys. J. 77, 2856-2856 (1999).</li><li>Chiou, P. C., Ohta, A. T., Wu, M. 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J., Garcia de Abajo, R. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Quidant+Nano-optical+Trapping+of+Rayleigh+Particles+Escherichia+coli+Bacteria+with+Resonant+Optical+Antennas.">Quidant Nano-optical Trapping of Rayleigh Particles Escherichia coli Bacteria with Resonant Optical Antennas.</a> Nano Letters. 9, 3387-3391 (2009).</li><li>Righini, M., Zelenina, A. S., Girard, C., Quidant, R. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Parallel+and+Selective+Trapping+in+a+Patterned+Plasmonic+Landscape.">Parallel and Selective Trapping in a Patterned Plasmonic Landscape.</a> Nature Physics. 3, 477-480 (2007).</li><li>Miao, X., Lin, L. 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诱捕这些方法的意义是，他们10μW/ 2微米的顺序 10 3 /μW的2微米到某处为了减少光的强度从某处持续诱捕所必需的。 10,11对这些技术的局限性黄金纳米粒子阵列经验暖气，必须克服的问题。为了克服这个问题，一个是电介质材料组成的二维光子晶体结构可以使用。这种结构在理论上可以产生在低光的强度和控制诱捕微型和纳米粒子在精确地控制输入偏振的旋转和位?...

<table border="1"><tr><td>材料名称</td><td>类型</td><td>公司</td><td>产品编号</td><td>评论</td></tr><tr><td> Axio上成像仪显微镜</td><td> D1M </td><td>蔡司</td><td> D1M </td><td>蔡司Axio上Imager.D1M </td></tr><tr><td>显微镜物镜</td><td> 50x/0.55 </td><td>蔡司</td><td></td><td> LD欧共体Epiplan - NEOFLUAR 50x/0.55高清DIC的</td></tr><tr><td>蔡司显微镜摄像机</td><td> AxioCam MRC </td><td>蔡司</td><td></td><td></td></tr><tr><td>氦氖激光器</td><td> 35兆瓦</td><td>研究光电</td><td></td><td></td></tr><tr><td>可变衰减器</td><td>连续可变ND </td><td> ThorLabs </td><td> NDC - 100C - 4M </td><td>对于调整显微镜强度</td></tr><tr><td>蔡司过滤器集</td><td>过滤器设置＃17 </td><td>蔡司</td><td> 488017-9901-000 </td><td>过滤器设置＃17 </td></tr><tr><td>显微镜载玻片</td><td>厚度为0.5毫米</td><td> VWR </td><td></td><td></td></tr><tr><td> 3T3小鼠细胞的细胞核</td><td></td><td colspan="2">弗雷德哈钦森癌症研究中心</td><td>存储为尽可能冷</td></tr><tr><td>吖啶橙染料</td><td></td><td colspan="2">弗雷德哈钦森癌症研究中心</td><td></td></tr><tr><td>牛血清白蛋白</td><td> 1到10的PBS的口粮</td><td colspan="2">弗雷德哈钦森癌症研究中心</td><td></td></tr><tr><td> 454 nm的聚苯乙烯乳胶球</td><td></td><td> Polysciences，公司</td><td></td><td></td></tr><tr><td>碳二亚胺盐酸盐（EDC） </td><td> 1 - 乙基-3 - （3 - dimethylaminopropyl） </td><td> G - Biosciences公司</td><td> BC25 - 1 </td><td></td></tr><tr><td>金（沉积） </td><td></td><td></td><td></td><td></td></tr><tr><td>反光裁定光栅</td><td></td><td>埃德蒙光学</td><td></td><td></td></tr><tr><td>磷酸盐缓冲液（PBS） </td><td>贝科的磷酸盐缓冲液（D - PBS）（1X） </td><td> Invitrogen公司</td><td> 14190-144 </td><td></td></tr></table>

utilization of plasmonic and photonic crystal nanostructures for enhanced micro- and nanoparticle manipulation

非破坏性的方法来操纵亚微米粒子的位置和方向将基础生物学研究的一个非常有用的工具。也许使用最广泛的物质力量来实现小颗粒的无创性操作已介（DEP）[1]然而，对自己的DEP缺乏通用性和精度，操作时所需的细胞，因为它是传统与固定电极。光镊，利用一个三维电磁场梯度小颗粒施加的力量，实现这一目标所需的通用性和精度。然而，这种方法的主要缺点是需要达到必要的武力来捕获一个粒子的辐射强度高可以破坏生物样品可诱捕和较低的光强度排序的解决方案光电镊子（OET），但OET的有小颗粒的精细操纵限制;。。被DEP的，基于技术，也使该解决方案的财产约束4 5 此视频文章将介绍两种方法，减少光学操纵活细胞所需的辐射强度，还描述了定向控制方法。第一种方法是使用一个随机的黄金纳米粒子（金纳米粒子）阵列，如图1所示样品基板电浆镊子。金纳米粒子阵列转换成本地化的表面等离子体（LSP），包括谐振偶极矩的事件光子辐射和产生的细胞液中的一个大梯度与图案的辐射场。表面等离子体增强诱捕Righini等和我们自己的造型的初步工作表明电浆基板所产生的领域，减少所需的初始强度，提高梯度场的粒子陷阱。6,7,8电浆方法允许罚款由于更高效的光能量转换成机械能和依赖偶极子辐射场的低光信号强度的椭球颗粒与细胞的定向控制。这些领域都显示在图2和图4和5中详细诱捕强度低。电浆镊子的主要问题是，LSP的产生大量的热量和诱捕只有二维。这种热产生对流流动和热泳可以强大到足以驱逐从陷阱的亚微米颗粒。9,10，我们将介绍第二种方法是利用周期性的电介质纳米结构非常有效地散射入射光成衍射模式，如图6所示11，理想的情况下，人会做出这种结构，电介质材料与电浆镊子，以避免相同的加热遇到的问题，但我们的做法镀铝光栅是作为一维周期介质纳米结构。虽然它不是一个半导体，它没有显著暖气经验和有效被困诱捕强度低的小颗粒，如图7所示。对准光栅基板的粒子概念验证的命题，一个2 - D光子晶体可以允许非球面微米大小的粒子的精确旋转10这些光学陷阱的效率增加，由于在纳米结构制作的增强的领域。本文。

Watch this Scientific Journal Video about Utilization of Plasmonic and Photonic Crystal Nanostructures for Enhanced Micro- and Nanoparticle Manipulation at JoVE.com

Utilization of Plasmonic and Photonic Crystal Nanostructures for Enhanced Micro- and Nanoparticle Manipulation

电浆镊子和光子晶体纳米结构的光学诱捕微型和纳米粒子的生产效率和方向的控制非常有用的增强。

为增强微型和纳米操纵利用电浆和光子晶体纳米结构

A method to manipulate the position and orientation of submicron particles nondestructively would be an incredibly useful tool for basic biological ...

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