Name: utilization of plasmonic and photonic crystal nanostructures for enhanced micro- and nanoparticle manipulation
Uploaded: 2011-09-27T12:00:00
Description: Watch this Scientific Journal Video about Utilization of Plasmonic and Photonic Crystal Nanostructures for Enhanced Micro- and Nanoparticle Manipulation at JoVE.com

हम भी Xiaoyu Miao और बेन विल्सन में वर्णित विधियों की सबसे अधिक विकसित करने के लिए धन्यवाद देना चाहूंगा. यह काम राष्ट्रीय विज्ञान फाउंडेशन (0,454,324 DBI) और राष्ट्रीय स्वास्थ्य संस्थान (EB005183 R21) द्वारा और Eck के लिए NIGMS से T32 एनआरएसए GM07270 PHS द्वारा वित्त पोषित किया गया था.

1. रैंडम Au Nanoparticle ऐरे 8,10,12,14 निर्माण <ol><li> Au nanoparticle सरणी पहले एक टेम्पलेट है कि 454 एनएम का मतलब व्यास के साथ बेतरतीब ढंग से adsorbed लाटेकस क्षेत्रों के एक घने परत से बना है बनाने के द्वारा बनाई है. यह 20 आसंजन परत के रू?...

<ol>
	<li>Jones, T. B. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=.">.</a> Electromechanics of Particles. , (1995).</li><li>Ashkin, A. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Optical+trapping+and+manipulation+of+neutral+particles+using+lasers.">Optical trapping and manipulation of neutral particles using lasers.</a> Proc. Natl. Acad. Sci. U.S.A. 94, 4853-4853 (1997).</li><li>Neuman, K. C., Chadd, E. H., Liou, G. F., Bergman, K., Block, S. M. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Characterization+of+photodamage+to+Escherichia+coli+in+optical+traps.">Characterization of photodamage to Escherichia coli in optical traps.</a> Biophys. J. 77, 2856-2856 (1999).</li><li>Chiou, P. C., Ohta, A. T., Wu, M. C. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Massively+parallel+manipulation+of+single+cells+and+microparticles+using+optical+images.">Massively parallel manipulation of single cells and microparticles using optical images.</a> Nature. 436, 370-370 (2005).</li><li>Hsu, H. Y., Ohta, A. T., Chiou, P. Y., Jamshidi, A., Nealea, S. L., Wua, M. C. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Phototransistor-based+optoelectronic+tweezers+for+dynamic+cell+manipulation+in+cell+culture+media.">Phototransistor-based optoelectronic tweezers for dynamic cell manipulation in cell culture media.</a> Lab Chip. 10, 165-172 (2010).</li><li>Righini, M., Ghenuche, P. S., Cherukulappurath, V., Myroshnychenko, F. J., Garcia de Abajo, R. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Quidant+Nano-optical+Trapping+of+Rayleigh+Particles+Escherichia+coli+Bacteria+with+Resonant+Optical+Antennas.">Quidant Nano-optical Trapping of Rayleigh Particles Escherichia coli Bacteria with Resonant Optical Antennas.</a> Nano Letters. 9, 3387-3391 (2009).</li><li>Righini, M., Zelenina, A. S., Girard, C., Quidant, R. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Parallel+and+Selective+Trapping+in+a+Patterned+Plasmonic+Landscape.">Parallel and Selective Trapping in a Patterned Plasmonic Landscape.</a> Nature Physics. 3, 477-480 (2007).</li><li>Miao, X., Lin, L. Y. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Large+dielectrophoresis+force+and+torque+induced+by+localized+surface+plasmon+resonance+of+a+cap-shaped+Au+nanoparticle+array.">Large dielectrophoresis force and torque induced by localized surface plasmon resonance of a cap-shaped Au nanoparticle array.</a> Opt. Lett. 32, 295-297 (2007).</li><li>Wilson, B. K. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=.">.</a> Manipulation of Nanoparticles and Biological Samples through Enhanced Optical Forces [dissertation]. , (2009).</li><li>Miao, X. Y., Wilson, B. K., Pun, S. H., Lin, L. Y. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Optical+manipulation+of+micron/submicron+sized+particles+and+biomolecules+through+plasmonics.">Optical manipulation of micron/submicron sized particles and biomolecules through plasmonics.</a> Optics Exp. 16, 13517-13525 (2008).</li><li>Wilson, B. K., Mentele, T., Bachar, S., Knouf, E., Bendoraite, A., Tewari, M., Pun, S. H., Lin, L. Y. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Nanostructure-enhanced+laser+tweezers+for+efficient+trapping+and+alignment+of+particles.">Nanostructure-enhanced laser tweezers for efficient trapping and alignment of particles.</a> Optics. Exp. 18, 16005-16013 (2010).</li><li>Miao, X., Wilson, B. K., Cao, G., Pun, S. H., Lin, L. Y. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Trapping+and+Rotation+of+Nanowires+Assisted+by+Surface+Plasmons.">Trapping and Rotation of Nanowires Assisted by Surface Plasmons.</a> IEEE Journal of Selected Topics in Quantum Electronics. 15, 1515-1520 (2009).</li><li>Miao, X. Y., Lin, L. Y. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Trapping+and+manipulation+of+biological+particles+through+a+plasmonic+platform.">Trapping and manipulation of biological particles through a plasmonic platform.</a> IEEE Journal of Selected Topics in Quantum Electronics. 13, 1655-1662 (2007).</li><li>Miao, X. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=.">.</a> Plasmonics for Micro/Nano Manipulation and Optofluidics [dissertation]. , (2008).</li></ol>

फँसाने के इन तरीकों का महत्व यह है कि वे 10 μW / 2 सुक्ष्ममापी के आदेश पर 10 3 μW / 2 सुक्ष्ममापी कहीं के आदेश पर ऑप्टिकल कहीं से निरंतर फँसाने के लिए आवश्यक तीव्रता में कमी 10,11 इन तकनीकों पर सीमाओं....

<table border="1"><tr><td> सामग्री नाम </td><td> टाइप </td><td> कंपनी </td><td> सूचि संख्या </td><td> टिप्पणी </td></tr><tr><td> Axio Imager माइक्रोस्कोप </td><td> D1M </td><td> Zeiss </td><td> D1M </td><td> Zeiss Axio Imager.D1M </td></tr><tr><td> माइक्रोस्कोप उद्देश्य </td><td> 50x/0.55 </td><td> Zeiss </td><td></td><td> LD चुनाव आयोग Epiplan - NEOFLUAR 50x/0.55 एच.डी. डीआईसी </td></tr><tr><td> Zeiss माइक्रोस्कोप कैमरा </td><td> AxioCam MRC </td><td> Zeiss </td><td></td><td></td></tr><tr><td> हीलियम नियॉन लेजर </td><td> 35 मेगावाट </td><td> अनुसंधान इलेक्ट्रो - प्रकाशिकी </td><td></td><td></td></tr><tr><td> परिवर्तनीय Attenuator </td><td> लगातार चर ND </td><td> Thorlabs </td><td> एनडीसी-100C-4M </td><td> खुर्दबीन तीव्रता को समायोजित करने के लिए </td></tr><tr><td> Zeiss फ़िल्टर सेट </td><td> फ़िल्टर सेट # 17 </td><td> Zeiss </td><td> 488017-9901-000 </td><td> फ़िल्टर सेट # 17 </td></tr><tr><td> खुर्दबीन स्लाइड </td><td> 0.5 मिमी मोटाई </td><td> VWR </td><td></td><td></td></tr><tr><td> 3T3 माउस सेल नाभिक </td><td></td><td colspan="2"> फ्रेड हचिंसन कैंसर रिसर्च सेंटर </td><td> के रूप में संभव के रूप में ठंडे की दुकान </td></tr><tr><td> Acridine ऑरेंज डाई </td><td></td><td colspan="2"> फ्रेड हचिंसन कैंसर रिसर्च सेंटर </td><td></td></tr><tr><td> गोजातीय सीरम albumin </td><td> पीबीएस में 1 से 10 राशन </td><td colspan="2"> फ्रेड हचिंसन कैंसर रिसर्च सेंटर </td><td></td></tr><tr><td> 454 एनएम polystyrene लाटेकस क्षेत्रों </td><td></td><td> Polysciences इंक, </td><td></td><td></td></tr><tr><td> carbodiimide हाइड्रोक्लोराइड (EDC) </td><td> 1-एथिल-3 (3 - dimethylaminopropyl) </td><td> G-बायोसाइंसेज </td><td> BC25-1 </td><td></td></tr><tr><td> सोना (बयान के लिए) </td><td></td><td></td><td></td><td></td></tr><tr><td> चिंतनशील शासन विवर्तन झंझरी </td><td></td><td> एडमंड प्रकाशिकी </td><td></td><td></td></tr><tr><td> फास्फेट खारा (पीबीएस) buffered </td><td> Dulbecco - फास्फेट buffered खारा (डी पीबीएस) (1X) </td><td> Invitrogen </td><td> 14190-144 </td><td></td></tr></table>

utilization of plasmonic and photonic crystal nanostructures for enhanced micro- and nanoparticle manipulation

A method to manipulate the position and orientation of submicron particles nondestructively would be an incredibly useful tool for basic biological research. Perhaps the most widely used physical force to achieve noninvasive manipulation of small particles has been dielectrophoresis(DEP).1 However, DEP on its own lacks the versatility and precision that are desired when manipulating cells since it is traditionally done with stationary electrodes. Optical tweezers, which utilize a three dimensional electromagnetic field gradient to exert forces on small particles, achieve this desired versatility and precision.2 However, a major drawback of this approach is the high radiation intensity required to achieve the necessary force to trap a particle which can damage biological samples.3 A solution that allows trapping and sorting with lower optical intensities are optoelectronic tweezers (OET) but OET's have limitations with fine manipulation of small particles; being DEP-based technology also puts constraint on the property of the solution.4,5
This video article will describe two methods that decrease the intensity of the radiation needed for optical manipulation of living cells and also describe a method for orientation control. The first method is plasmonic tweezers which use a random gold nanoparticle (AuNP) array as a substrate for the sample as shown in Figure 1. The AuNP array converts the incident photons into localized surface plasmons (LSP) which consist of resonant dipole moments that radiate and generate a patterned radiation field with a large gradient in the cell solution. Initial work on surface plasmon enhanced trapping by Righini et al and our own modeling have shown the fields generated by the plasmonic substrate reduce the initial intensity required by enhancing the gradient field that traps the particle.6,7,8 The plasmonic approach allows for fine orientation control of ellipsoidal particles and cells with low optical intensities because of more efficient optical energy conversion into mechanical energy and a dipole-dependent radiation field. These fields are shown in figure 2 and the low trapping intensities are detailed in figures 4 and 5. The main problems with plasmonic tweezers are that the LSP's generate a considerable amount of heat and the trapping is only two dimensional. This heat generates convective flows and thermophoresis which can be powerful enough to expel submicron particles from the trap.9,10 The second approach that we will describe is utilizing periodic dielectric nanostructures to scatter incident light very efficiently into diffraction modes, as shown in figure 6.11 Ideally, one would make this structure out of a dielectric material to avoid the same heating problems experienced with the plasmonic tweezers but in our approach an aluminum-coated diffraction grating is used as a one-dimensional periodic dielectric nanostructure. Although it is not a semiconductor, it did not experience significant heating and effectively trapped small particles with low trapping intensities, as shown in figure 7. Alignment of particles with the grating substrate conceptually validates the proposition that a 2-D photonic crystal could allow precise rotation of non-spherical micron sized particles.10 The efficiencies of these optical traps are increased due to the enhanced fields produced by the nanostructures described in this paper.

Watch this Scientific Journal Video about Utilization of Plasmonic and Photonic Crystal Nanostructures for Enhanced Micro- and Nanoparticle Manipulation at JoVE.com

Utilization of Plasmonic and Photonic Crystal Nanostructures for Enhanced Micro- and Nanoparticle Manipulation

Plasmonic चिमटी और photonic क्रिस्टल nanostructures के ऑप्टिकली माइक्रो और नैनो कणों को फँसाने की दक्षता और ओरिएंटेशन नियंत्रण में उपयोगी संवर्द्धन का उत्पादन करने के लिए दिखाए जाते हैं.

Plasmonic और Photonic क्रिस्टल nanostructures की बढ़ी माइक्रो Nanoparticle और हेरफेर के लिए उपयोगिता

A method to manipulate the position and orientation of submicron particles nondestructively would be an incredibly useful tool for basic biological ...

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