Name: utilization of plasmonic and photonic crystal nanostructures for enhanced micro- and nanoparticle manipulation
Uploaded: 2011-09-27T12:00:00
Description: Watch this Scientific Journal Video about Utilization of Plasmonic and Photonic Crystal Nanostructures for Enhanced Micro- and Nanoparticle Manipulation at JoVE.com

Мы также хотели бы поблагодарить Сяоюй Мяо и Бена Уилсона для разработки большинства методов, описанных в пределах. Эта работа финансировалась Национальным научным фондом (DBI 0454324) и Национального института здоровья (R21 EB005183) и PHS НРСА T32 GM07270 от NIGMS к ECK.

1. Случайные наночастиц Au массива Изготовление 8,10,12,14 <ol><li> Массивов наночастиц Au формируется сначала создается шаблон, который сделан из плотного слоя адсорбированных случайно латексных шаров с средним диаметром 454 нм. Это достигается за счет испарения первого золота на стекло ?...

<ol>
	<li>Jones, T. B. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=.">.</a> Electromechanics of Particles. , (1995).</li><li>Ashkin, A. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Optical+trapping+and+manipulation+of+neutral+particles+using+lasers.">Optical trapping and manipulation of neutral particles using lasers.</a> Proc. Natl. Acad. Sci. U.S.A. 94, 4853-4853 (1997).</li><li>Neuman, K. C., Chadd, E. H., Liou, G. F., Bergman, K., Block, S. M. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Characterization+of+photodamage+to+Escherichia+coli+in+optical+traps.">Characterization of photodamage to Escherichia coli in optical traps.</a> Biophys. J. 77, 2856-2856 (1999).</li><li>Chiou, P. C., Ohta, A. T., Wu, M. C. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Massively+parallel+manipulation+of+single+cells+and+microparticles+using+optical+images.">Massively parallel manipulation of single cells and microparticles using optical images.</a> Nature. 436, 370-370 (2005).</li><li>Hsu, H. Y., Ohta, A. T., Chiou, P. Y., Jamshidi, A., Nealea, S. L., Wua, M. C. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Phototransistor-based+optoelectronic+tweezers+for+dynamic+cell+manipulation+in+cell+culture+media.">Phototransistor-based optoelectronic tweezers for dynamic cell manipulation in cell culture media.</a> Lab Chip. 10, 165-172 (2010).</li><li>Righini, M., Ghenuche, P. S., Cherukulappurath, V., Myroshnychenko, F. J., Garcia de Abajo, R. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Quidant+Nano-optical+Trapping+of+Rayleigh+Particles+Escherichia+coli+Bacteria+with+Resonant+Optical+Antennas.">Quidant Nano-optical Trapping of Rayleigh Particles Escherichia coli Bacteria with Resonant Optical Antennas.</a> Nano Letters. 9, 3387-3391 (2009).</li><li>Righini, M., Zelenina, A. S., Girard, C., Quidant, R. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Parallel+and+Selective+Trapping+in+a+Patterned+Plasmonic+Landscape.">Parallel and Selective Trapping in a Patterned Plasmonic Landscape.</a> Nature Physics. 3, 477-480 (2007).</li><li>Miao, X., Lin, L. Y. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Large+dielectrophoresis+force+and+torque+induced+by+localized+surface+plasmon+resonance+of+a+cap-shaped+Au+nanoparticle+array.">Large dielectrophoresis force and torque induced by localized surface plasmon resonance of a cap-shaped Au nanoparticle array.</a> Opt. Lett. 32, 295-297 (2007).</li><li>Wilson, B. K. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=.">.</a> Manipulation of Nanoparticles and Biological Samples through Enhanced Optical Forces [dissertation]. , (2009).</li><li>Miao, X. Y., Wilson, B. K., Pun, S. H., Lin, L. Y. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Optical+manipulation+of+micron/submicron+sized+particles+and+biomolecules+through+plasmonics.">Optical manipulation of micron/submicron sized particles and biomolecules through plasmonics.</a> Optics Exp. 16, 13517-13525 (2008).</li><li>Wilson, B. K., Mentele, T., Bachar, S., Knouf, E., Bendoraite, A., Tewari, M., Pun, S. H., Lin, L. Y. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Nanostructure-enhanced+laser+tweezers+for+efficient+trapping+and+alignment+of+particles.">Nanostructure-enhanced laser tweezers for efficient trapping and alignment of particles.</a> Optics. Exp. 18, 16005-16013 (2010).</li><li>Miao, X., Wilson, B. K., Cao, G., Pun, S. H., Lin, L. Y. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Trapping+and+Rotation+of+Nanowires+Assisted+by+Surface+Plasmons.">Trapping and Rotation of Nanowires Assisted by Surface Plasmons.</a> IEEE Journal of Selected Topics in Quantum Electronics. 15, 1515-1520 (2009).</li><li>Miao, X. Y., Lin, L. Y. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Trapping+and+manipulation+of+biological+particles+through+a+plasmonic+platform.">Trapping and manipulation of biological particles through a plasmonic platform.</a> IEEE Journal of Selected Topics in Quantum Electronics. 13, 1655-1662 (2007).</li><li>Miao, X. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=.">.</a> Plasmonics for Micro/Nano Manipulation and Optofluidics [dissertation]. , (2008).</li></ol>

Значение этих методов захвата является то, что они снижают интенсивности оптического необходимых для устойчивого захвата откуда-то порядка 10 3 мкВт / мкм 2, чтобы где-то порядка 10 мкВт / мкм 2. 10,11 ограничения на эти методы в том, что золотые наночастицы массив опыта отопл?...

<table border="1"><tr><td> Материал Имя </td><td> Тип </td><td> Компания </td><td> Номер в каталоге </td><td> Комментарий </td></tr><tr><td> Axio Imager микроскоп </td><td> D1M </td><td> Zeiss </td><td> D1M </td><td> Zeiss Axio Imager.D1M </td></tr><tr><td> Объектива микроскопа </td><td> 50x/0.55 </td><td> Zeiss </td><td></td><td> Л. Д. ЕС Epiplan - NEOFLUAR 50x/0.55 HD DIC </td></tr><tr><td> Микроскоп Zeiss камера </td><td> AxioCam MRC </td><td> Zeiss </td><td></td><td></td></tr><tr><td> Гелий неонового лазера </td><td> 35 мВт </td><td> Исследования Электро-Оптика </td><td></td><td></td></tr><tr><td> Переменный аттенюатор </td><td> Непрерывно Переменная ND </td><td> ThorLabs </td><td> НДЦ-100С-4М </td><td> Для регулировки интенсивности микроскопом </td></tr><tr><td> Zeiss набора фильтров </td><td> Фильтры Set # 17 </td><td> Zeiss </td><td> 488017-9901-000 </td><td> Фильтры Set # 17 </td></tr><tr><td> Микроскоп Слайды </td><td> Толщиной 0,5 мм </td><td> VWR </td><td></td><td></td></tr><tr><td> 3T3 ядрах клеток мыши </td><td></td><td colspan="2"> Фреда Хатчинсона онкологический научный центр </td><td> Магазин так холодно, как возможно </td></tr><tr><td> Акридинового оранжевого красителя </td><td></td><td colspan="2"> Фреда Хатчинсона онкологический научный центр </td><td></td></tr><tr><td> Бычьего сывороточного альбумина </td><td> От 1 до 10 паек в PBS </td><td colspan="2"> Фреда Хатчинсона онкологический научный центр </td><td></td></tr><tr><td> 454 нм сферы полистирола латекса </td><td></td><td> Polysciences, Inc </td><td></td><td></td></tr><tr><td> карбодиимида гидрохлорид (EDC) </td><td> 1-этил-3-(3-диметиламинопропил) </td><td> G-биологических наук </td><td> BC25-1 </td><td></td></tr><tr><td> золото (для осаждения) </td><td></td><td></td><td></td><td></td></tr><tr><td> Отражающие исключено дифракционной решетки </td><td></td><td> Эдмунд оптики </td><td></td><td></td></tr><tr><td> Фосфатно-солевым буфером (PBS) </td><td> Дульбеко фосфатно-солевом буфере (D-би-эс) (1X) </td><td> Invitrogen </td><td> 14190-144 </td><td></td></tr></table>

utilization of plasmonic and photonic crystal nanostructures for enhanced micro- and nanoparticle manipulation

A method to manipulate the position and orientation of submicron particles nondestructively would be an incredibly useful tool for basic biological research. Perhaps the most widely used physical force to achieve noninvasive manipulation of small particles has been dielectrophoresis(DEP).1 However, DEP on its own lacks the versatility and precision that are desired when manipulating cells since it is traditionally done with stationary electrodes. Optical tweezers, which utilize a three dimensional electromagnetic field gradient to exert forces on small particles, achieve this desired versatility and precision.2 However, a major drawback of this approach is the high radiation intensity required to achieve the necessary force to trap a particle which can damage biological samples.3 A solution that allows trapping and sorting with lower optical intensities are optoelectronic tweezers (OET) but OET's have limitations with fine manipulation of small particles; being DEP-based technology also puts constraint on the property of the solution.4,5
This video article will describe two methods that decrease the intensity of the radiation needed for optical manipulation of living cells and also describe a method for orientation control. The first method is plasmonic tweezers which use a random gold nanoparticle (AuNP) array as a substrate for the sample as shown in Figure 1. The AuNP array converts the incident photons into localized surface plasmons (LSP) which consist of resonant dipole moments that radiate and generate a patterned radiation field with a large gradient in the cell solution. Initial work on surface plasmon enhanced trapping by Righini et al and our own modeling have shown the fields generated by the plasmonic substrate reduce the initial intensity required by enhancing the gradient field that traps the particle.6,7,8 The plasmonic approach allows for fine orientation control of ellipsoidal particles and cells with low optical intensities because of more efficient optical energy conversion into mechanical energy and a dipole-dependent radiation field. These fields are shown in figure 2 and the low trapping intensities are detailed in figures 4 and 5. The main problems with plasmonic tweezers are that the LSP's generate a considerable amount of heat and the trapping is only two dimensional. This heat generates convective flows and thermophoresis which can be powerful enough to expel submicron particles from the trap.9,10 The second approach that we will describe is utilizing periodic dielectric nanostructures to scatter incident light very efficiently into diffraction modes, as shown in figure 6.11 Ideally, one would make this structure out of a dielectric material to avoid the same heating problems experienced with the plasmonic tweezers but in our approach an aluminum-coated diffraction grating is used as a one-dimensional periodic dielectric nanostructure. Although it is not a semiconductor, it did not experience significant heating and effectively trapped small particles with low trapping intensities, as shown in figure 7. Alignment of particles with the grating substrate conceptually validates the proposition that a 2-D photonic crystal could allow precise rotation of non-spherical micron sized particles.10 The efficiencies of these optical traps are increased due to the enhanced fields produced by the nanostructures described in this paper.

Watch this Scientific Journal Video about Utilization of Plasmonic and Photonic Crystal Nanostructures for Enhanced Micro- and Nanoparticle Manipulation at JoVE.com

Utilization of Plasmonic and Photonic Crystal Nanostructures for Enhanced Micro- and Nanoparticle Manipulation

Плазмонных пинцет и фотонных кристаллов наноструктур показаны для производства полезных улучшений в эффективности и ориентации контроль оптически захвата микро-и нано-частиц.

Использование плазмонных наноструктур и фотонных кристаллов для работы с улучшенными микро-и наночастиц Манипуляция

A method to manipulate the position and orientation of submicron particles nondestructively would be an incredibly useful tool for basic biological ...

Research

JoVE Journal

Bioengineering

Planar and Three-Dimensional Printing of Conductive Inks

Плоские и трехмерной печати проводящей краски

Printed electronics rely on low-cost, large-area fabrication routes to create flexible or multidimensional electronic, optoelectronic, and biomedical ...

Synthesis, Assembly, and Characterization of Monolayer Protected Gold Nanoparticle Films for Protein Monolayer Electrochemistry

Синтез, монтаж, и характеристика монослоя охраняемые пленок золота наночастицы для Белки электрохимии монослоя

Colloidal gold nanoparticles protected with alkanethiolate ligands called monolayer protected gold clusters (MPCs) are synthesized and subsequently ...

Evaluation of Polymeric Gene Delivery Nanoparticles by Nanoparticle Tracking Analysis and High-throughput Flow Cytometry

Оценка Полимерные доставки генов наночастиц наночастиц Анализ отслеживания и высокой пропускной проточной цитометрии

Non-viral gene delivery using polymeric nanoparticles has emerged as an attractive approach for gene therapy to treat genetic diseases1 and as a ...

Micro-particle Image Velocimetry for Velocity Profile Measurements of Micro Blood Flows

Micro-Измерение скорости Изображения Частиц для профиля скорости Измерения Micro приливает кровь

Micro-particle image velocimetry (&mu;PIV) is used to visualize paired images of micro particles seeded in blood flows. The images are cross-correlated to ...

ECM Protein Nanofibers and Nanostructures Engineered Using Surface-initiated Assembly

ECM Белковые Нановолокон и наноструктуры Инженерная Использование Поверхность по инициативе Ассамблеи

The extracellular matrix (ECM) in tissues is synthesized and assembled by cells to form a 3D fibrillar, protein network with tightly regulated fiber ...

Preparation of Monodomain Liquid Crystal Elastomers and Liquid Crystal Elastomer Nanocomposites

Получение Monodomain жидкокристаллический эластомеров и жидкокристаллический эластомерных нанокомпозитов

LCEs are shape-responsive materials with fully reversible shape change and potential applications in medicine, tissue engineering, artificial muscles, and ...

Techniques for the Evolution of Robust Pentose-fermenting Yeast for Bioconversion of Lignocellulose to Ethanol

Методы эволюции Robust пентозы брожения дрожжей для биоконверсии лигноцеллюлозы к этанолу

Lignocellulosic biomass is an abundant, renewable feedstock useful for production of fuel-grade ethanol and other bio-products. Pretreatment and enzyme ...

Microfluidic Buffer Exchange for Interference-free Micro/Nanoparticle Cell Engineering

Микрожидкостных буфера обмена для Помехоустойчивое Micro / клеточной инженерии наночастиц

Engineering cells with active-ingredient-loaded micro/nanoparticles (NPs) is becoming an increasingly popular method to enhance native therapeutic ...

Targeted Plasma Membrane Delivery of a Hydrophobic Cargo Encapsulated in a Liquid Crystal Nanoparticle Carrier

Целенаправленное плазматической мембраны Поставка гидрофобной Cargo инкапсулированы в жидком кристалле наночастицы Carrier

The controlled delivery of drug/imaging agents to cells is critical for the development of therapeutics and for the study of cellular signaling processes. ...

Synthesis of Biocompatible Liquid Crystal Elastomer Foams as Cell Scaffolds for 3D Spatial Cell Cultures

Синтез биосовместимых жидкого кристалла эластомерных пен, как Cell Каркасы для 3D-пространственных культур клеток

Here, we present a step-by-step preparation of a 3D, biodegradable, foam-like cell scaffold. These scaffolds were prepared by cross-linking star block ...