Name: chronic constriction of the sciatic nerve and pain hypersensitivity testing in rats
Uploaded: 2012-03-13T12:00:00
Description: Watch this Scientific Journal Video about Chronic Constriction of the Sciatic Nerve and Pain Hypersensitivity Testing in Rats at JoVE.com

Forfatterne vil gerne anerkende den oprindelige beskrivelse af de fælles konsulære instrukser model af Bennett og Xie (1988). Dette arbejde blev støttet delvist af tilskud fra National Health og Medical Research Council of Australia (ID # 568.637) og NSW Kontoret for videnskab og medicinsk forskning til GMT.

1. Iskiasnerven Kronisk Konstriktion Aseptiske skal anvendes til den kirurgiske procedure. Desinficer den kirurgiske arbejde overfladen med 70% ethanol og på forhånd forberede sterile instrumenter (f.eks fine saks, pincet og retraktor), gazer, hæfteklammer og podninger ved autoklavering. For flere operationer, rengøre og resterilise instrumenter med 70% ethanol eller en tør perle sterilisator mellem rotter. En kirurgisk maske, hår motorhjelm og sterile handsker bør anvendes. <p clas...

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CCI er en almindeligt anvendt perifer nerveskade model for kronisk neuropatisk smerte. Det er forholdsvis enkelt at udføre, og producerer robust og stabil smerte hypersensitivitet i mindst en måned efter skade. Efter CCI udviser rotter unormal kropsholdning af tilskadekomne bagpote (tæer holdt sammen og plantar-bøjet og pote vendt), såvel som gentagen rystning, bevogtning og slikke af de sårede bagpote tyder på tilstedeværelsen af spontan smerte 9. Ud over sensorisk dysfunktion, har adskillige forsker...

<table border="1"><tbody><tr><td> Navn på reagenset / udstyr </td><td> Firma </td><td> Katalog nummer </td><td> Kommentarer </td></tr><tr><td> Krom gut </td><td> Ethicon </td><td> G-211 </td><td> 4-0 tykkelse </td></tr><tr><td> Isofluran </td><td> Delvet Pty Ltd, Seven Hills, NSW </td><td> n / a </td><td></td></tr><tr><td> Mersilk </td><td> Ethicon </td><td> W-580 </td><td> 5-0 tykkelse </td></tr><tr><td> Autoclip </td><td> Becton Dickinson </td><td> 427631 </td><td> 9 mm rustfrit stål </td></tr><tr><td> Riodine </td><td> Orion </td><td> R1000802F </td><td> 1% w / v iod </td></tr><tr><td> Termisk plantar analgesi instrument </td><td> Ugo Basile </td><td> 37370 </td><td></td></tr><tr><td> Dynamisk plantar aesthesiometer </td><td> Ugo Basile </td><td> 37400 </td><td></td></tr></tbody></table>

chronic constriction of the sciatic nerve and pain hypersensitivity testing in rats

Kronisk neuropatisk smerte, der skyldes skade på det centrale eller perifere nervesystem, er et fremherskende og invaliderende lidelse, der påvirker 7-18% af befolkningen 1,2. Symptomerne omfatter spontan (prikken, brændende, elektrisk stød lignende) smerter, dysæstesi, paræstesi, allodyni (smerter som følge af normalt ikke-smertefulde stimuli) og hyperalgesi (øget respons på smertefulde stimuli). De sensoriske symptomer er co-morbide med adfærdsmæssige handicap, såsom søvnløshed og depression. At studere kronisk neuropatisk smerte adskillige dyremodeller efterligner perifer nerveskade blevet udviklet, et af de benyttede mest er Bennett og Xie s (1988) ensidig ischiasnerven kronisk konstriktion skade (CCI) 3 (figur 1). Her præsenterer vi en metode til at udføre CCI og test smerte overfølsomhed. CCI udføres under bedøvelse, med iskiasnerven på den ene side udsættes for ved at gøre somkin indsnit, og skære gennem bindevæv mellem gluteus superficialis og biceps femoris muskler. Fire krom gut ligaturer er bundet løst omkring iskiasnerven på 1 mm mellemrum, bare occlude, men ikke arrestere epineural blodgennemstrømning. Såret lukkes med suturer i muskel og hæfteklammer i huden. Dyret får derefter lov at komme sig kirurgi for 24 timer før smerten overfølsomhed testning påbegyndes. For adfærdsmæssig testning rotterne placeret i testapparatet, og får lov til at vænne sig til testproceduren. Det testede område er midt-plantar overflade bagpote (figur 2), der falder inden for iskiasnerven distribution. Mekanisk tilbagetrækning tærskel vurderes ved mekanisk at stimulere både sårede og skadede bagpoter ved hjælp af et elektronisk dynamisk plantar von Frey aesthesiometer eller manuel von Frey hår 4. Den mekaniske tilbagetrækning tærskel er det maksimale pression(I gram), der udløser tilbagetrækning af poten. Til måling af termisk tilbagetrækning latens, først beskrevet af Hargreaves et al (1988), er bagpote udsættes for en stråle af strålevarme gennem et transparent glas-overfladen med en plantart analgesi meter 5,6. Tilbagetrækningen latenstid for varme stimulus registreres som tidspunktet for potetilbagetrækning både skadede og ubeskadiget bagpoter. Efter CCI, mekanisk tilbagetrækning tærskel, samt termisk tilbagetrækning latenstid i den skadede pote er både væsentligt reduceret i forhold til baseline målinger og skadede pote (Figur 3). CCI-modellen af ​​perifer nerveskade kombineret med smerte overfølsomhed test tilvejebringer et modelsystem til at undersøge effektiviteten af ​​potentielle terapeutiske midler til at modificere kronisk neuropatisk smerte. I vores laboratorium, benytter vi CCI sammen med termisk og mekanisk følsomhed af de bagpoter at undersøge betydningen af ​​neuro-immune interaktioner i patogenesenog behandling af neuropatisk smerte.

Watch this Scientific Journal Video about Chronic Constriction of the Sciatic Nerve and Pain Hypersensitivity Testing in Rats at JoVE.com

Chronic Constriction of the Sciatic Nerve and Pain Hypersensitivity Testing in Rats

På grund af enkelheden af ​​kirurgi og robust adfærdsmæssige mål, kronisk konstriktion af iskiasnerven er en af ​​de fremtrædende dyremodeller for neuropatisk smerte. Inden for 24 timer efter kirurgi, smerte overfølsomhed etableret og kan måles kvantitativt ved hjælp af en von Frey aesthesiometer (mekanisk test) og plantar analgesi meter (termisk test).

Kronisk Konstriktion af iskiasnerven og Smerter Overfølsomhed test i rotter

Chronic neuropathic pain, resulting from damage to the central or peripheral nervous system, is a prevalent and debilitating condition, affecting 7-18% of ...

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