Name: chronic constriction of the sciatic nerve and pain hypersensitivity testing in rats
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De auteurs willen graag de oorspronkelijke beschrijving van de CCI-model door Bennett en Xie (1988) erkennen. Dit werk werd gedeeltelijk ondersteund door subsidies van de National Health and Medical Research Council van Australië (ID # 568637) en de NSW Bureau voor Wetenschap en Medisch Onderzoek naar GMT.

1. Heupzenuw Chronische Vernauwing Aseptisch worden gebruikt voor de chirurgische procedure. Ontsmet de chirurgische werkblad met 70% ethanol en voor te bereiden op voorhand steriele instrumenten (bv., fijn schaar, pincet, en oprolmechanisme), gaasjes, nietjes en swabs in de autoclaaf. Voor meerdere operaties, reinigen en opnieuw steriliseren instrumenten met 70% ethanol of een droge korrel sterilisator tussen de ratten. Een chirurgisch masker, haar motorkap en steriele handschoenen te worden ...

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CCI is een veel gebruikte perifere zenuwschade model van chronische neuropathische pijn. Het is relatief eenvoudig uit te voeren, en produceert robuuste en stabiele pijn overgevoeligheid ten minste een maand na verwonding. Naar aanleiding van CCI, ratten vertonen abnormale houding van de gewonde achterpoot (tenen bij elkaar gehouden en plantaire-gebogen en poot eversie), maar ook herhaaldelijk schudden, bewaking en likken van de gewonde achterpoot suggereert de aanwezigheid van spontane pijn 9. Naast de senso...

<table border="1"><tbody><tr><td> Naam van het reagens / apparatuur </td><td> Vennootschap </td><td> Catalogusnummer </td><td> Reacties </td></tr><tr><td> Chroomzuur darm </td><td> Ethicon </td><td> G-211 </td><td> 4-0 dikte </td></tr><tr><td> Isofluraan </td><td> Delvet Pty Ltd, Seven Hills, Zuid-Holland </td><td> n / a </td><td></td></tr><tr><td> Mersilk </td><td> Ethicon </td><td> W-580 </td><td> 5-0 dikte </td></tr><tr><td> Autoclip </td><td> Becton Dickinson </td><td> 427631 </td><td> 9 mm roestvrij staal </td></tr><tr><td> Riodine </td><td> Orion </td><td> R1000802F </td><td> 1% w / v jodium </td></tr><tr><td> Thermische plantaire analgesie instrument </td><td> Ugo Basile </td><td> 37370 </td><td></td></tr><tr><td> Dynamische plantaire aesthesiometer </td><td> Ugo Basile </td><td> 37400 </td><td></td></tr></tbody></table>

chronic constriction of the sciatic nerve and pain hypersensitivity testing in rats

Chronische neuropathische pijn, als gevolg van beschadiging van het centrale of perifere zenuwstelsel, is een wijdverspreide en slopende aandoening, die 7-18% van de bevolking 1,2. Symptomen zijn onder meer spontane (tintelingen, branderig gevoel, elektrische schok ed) pijn, dysesthesie, paresthesie, allodynie (pijn als gevolg van normaal niet-pijnlijke stimuli) en hyperalgesie (een verhoogde respons op pijnlijke stimuli). De sensorische symptomen zijn co-morbide met gedragsproblemen een handicap, zoals slapeloosheid en depressie. Om chronische neuropathische pijn te bestuderen verschillende diermodellen het nabootsen van perifere zenuwschade zijn ontwikkeld, een van de meest gebruikte is Bennett en Xie&#39;s (1988) eenzijdige heupzenuw chronische vernauwing letsel (CCI) 3 (figuur 1). Hier presenteren we een methode voor het uitvoeren van CCI en testen pijn overgevoeligheid. CCI wordt uitgevoerd onder narcose, waarbij de heupzenuw aan de ene kant worden blootgesteld door het maken van dekin incisie, en dwars door het bindweefsel tussen de gluteus superficialis en biceps femoris spieren. Vier chroomzuur darm ligaturen zijn losjes gebonden rond de heupzenuw bij 1 mm intervallen, om er maar af te sluiten, maar niet arresteren epineural doorbloeding. De wond gesloten met hechtingen in de spier en nietjes in de huid. Het dier wordt dan mag van een operatie herstellen 24 uur voor pijn overgevoeligheid test gestart. Voor gedrags-testen, worden ratten geplaatst in de testapparatuur en mogen wennen aan de testprocedure. Getest Het gebied is het midden van de voetzool van de achterpoot (figuur 2), die valt binnen de heupzenuw distributie. Mechanische terugtrekking drempel wordt berekend door het mechanisch het stimuleren van zowel gewonden en niet-gewonde hindpaws met behulp van een elektronische dynamische plantaire von Frey aesthesiometer of handmatige von Frey haren 4. De mechanische onttrekkingsdrempel is de uitgeoefende maximale druk(In gram) die leidt tot poot terugtrekken. Voor het meten van thermische terugtrekking latentie eerst beschreven door Hargreaves et al. (1988), wordt de achterpoot blootgesteld aan een balk van stralingswarmte door een glazen oppervlak met een plantairflexie analgesie meter 5,6. De intrekking latency aan de warmte stimulus wordt geregistreerd als de tijd voor poot terugtrekking in zowel gewonden en niet gewonde hindpaws. Naar aanleiding van CCI, mechanische onttrekkingsdrempel, evenals thermische terugtrekking latency in de geblesseerde poot zijn beide aanzienlijk verminderd, vergeleken met nulmetingen en de niet gewonde poot (figuur 3). De CCI-model van perifere zenuwschade in combinatie met pijn overgevoeligheid testen biedt een modelsysteem om de effectiviteit van potentiële therapeutische middelen te onderzoeken om chronische neuropathische pijn te wijzigen. In ons laboratorium, maken wij gebruik van CCI naast thermische en mechanische gevoeligheid van de hindpaws om de rol van neuro-immuun interacties te onderzoeken in de pathogeneseen de behandeling van neuropathische pijn.

Watch this Scientific Journal Video about Chronic Constriction of the Sciatic Nerve and Pain Hypersensitivity Testing in Rats at JoVE.com

Chronic Constriction of the Sciatic Nerve and Pain Hypersensitivity Testing in Rats

Door de eenvoud van de ingreep en de robuuste gedrag dat eruit voortkomt, chronische vernauwing van de nervus ischiadicus is een van de vooraanstaande dierlijke modellen van neuropathische pijn. Binnen 24 uur na een operatie, wordt de pijn overgevoeligheid vastgesteld en kan kwantitatief gemeten worden met een von Frey aesthesiometer (mechanische test) en plantaire analgesie meter (thermische test).

Chronische Vernauwing van de nervus ischiadicus en Pijn Overgevoeligheid Testen bij ratten

Chronic neuropathic pain, resulting from damage to the central or peripheral nervous system, is a prevalent and debilitating condition, affecting 7-18% of ...

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