Name: chronic constriction of the sciatic nerve and pain hypersensitivity testing in rats
Uploaded: 2012-03-13T12:00:00
Description: Watch this Scientific Journal Video about Chronic Constriction of the Sciatic Nerve and Pain Hypersensitivity Testing in Rats at JoVE.com

Yazarlar Bennett ve Xie (1988) tarafından CCİ modelin orijinal tanımı kabul etmek istiyorum. Bu çalışma Bilim ve GMT Tıbbi Araştırma Ulusal Sağlık ve Avustralya Tıbbi Araştırma Konseyi (ID # 568637) ve NSW hibe tarafından kısmen desteklenmiştir.

1. Siyatik Sinir Kronik daraltma Aseptik teknikler cerrahi işlem için kullanılmalıdır. % 70 etanol ile cerrahi iş yüzeyi dezenfekte edin ve otoklavlayarak önceden steril aletler (örn., ince makas, forseps ve retraktör), tüller, zımba ve bezlerden de hazırlar. Çoklu operasyonlar için, temiz ve% 70 etanol ya da sıçanlar arasında kuru bir boncuk sterilizanlar ile resterilise aletleri. Bir cerrahi maske, saç kaporta ve steril eldiven giyilmelidir. Si...

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CCİ kronik nöropatik ağrı yaygın olarak kullanılan periferik sinir yaralanması modelidir. Bunu gerçekleştirmek için oldukça basit ve yaralanma sonrası en az bir ay için sağlam ve kararlı bir ağrı aşırı duyarlılık üretir. CCİ takiben sıçan yaralı sıçanın sol arka ayağını (parmakları bir arada tutan ve plantar fleksiyon ve pençe everte) yanı sıra, sallayarak koruma ve spontan ağrı 9 varlığını düşündüren yaralı sıçanın sol arka ayağını yalamaya tekrarlanan an...

<table border="1"><tbody><tr><td> Reaktif / ekipman Adı </td><td> Şirket </td><td> Katalog numarası </td><td> Yorumlar </td></tr><tr><td> Kromik gut </td><td> Ethicon </td><td> G-211 </td><td> 4-0 kalınlığı </td></tr><tr><td> Izofluran </td><td> Delvet Pty Ltd, Seven Hills, NSW </td><td> n / a </td><td></td></tr><tr><td> Mersilk </td><td> Ethicon </td><td> W-580 </td><td> 5-0 kalınlığı </td></tr><tr><td> AutoCLIP </td><td> Becton Dickinson </td><td> 427631 </td><td> 9 mm paslanmaz çelik </td></tr><tr><td> Riodine </td><td> Orion </td><td> R1000802F </td><td> % 1 w / v iyot </td></tr><tr><td> Termal plantar analjezi cihazı </td><td> Ugo Basile </td><td> 37370 </td><td></td></tr><tr><td> Dinamik plantar aesthesiometer </td><td> Ugo Basile </td><td> 37400 </td><td></td></tr></tbody></table>

chronic constriction of the sciatic nerve and pain hypersensitivity testing in rats

Kronik nöropatik ağrı, merkezi ya da periferal sinir sisteminin hasar görmesi sonucu, nüfusun 1,2% 7-18 etkileyen, yaygın bir ve zayıflatıcı bir durumdur. Belirtileri spontan (gibi karıncalanma, yanma, elektrik çarpması) ağrı, disestezi, parestezi, allodini (ağrı normal olmayan ağrılı uyaranlar kaynaklanan) ve hiperaljezi (ağrılı uyaranlara artmış yanıt) içerir. Duysal semptomlar uykusuzluk ve depresyon gibi davranışsal özürlü ile komorbid bulunmaktadır. Kronik nöropatik ağrı çalışmak için periferik sinir yaralanması taklit eden birçok hayvan modellerinde geliştirilmiş olup, en yaygın olarak kullanılan bir Bennett ve Xie (1988) tek taraflı siyatik sinir kronik daralma yaralanma (CCI) 3 (Şekil 1). Burada CC ve test ağrısı aşırı duyarlılık gerçekleştirmek için bir yöntem sunulmaktadır. CCİ olarak yaparak maruz kalan bir tarafta siyatik sinir ile, anestezi altında yapılırkin insizyon ve gluteus süperfisiyalis ve biseps femoris kaslar arasındaki bağ dokusu ile kesme. Dört kromik katgüt Ligatürleri sadece kapamak için, 1 mm aralıklarla siyatik sinir etrafındaki gevşek bağlanmış ama epinöral kan akışını tutuklama değildir. Yara deri kas ve mahsullerindeki dikişler ile kapatılır. Hayvan daha sonra ağrı aşırı duyarlılık testleri başlamadan önce 24 saat için ameliyatın izin verilir. Davranış testi için, sıçanlarda test cihazları yerleştirilir ve test prosedürü alışmakta izin verilir. Test alanı siyatik sinir dağılımında içinde düşer sıçanın sol arka ayağını orta plantar (Şekil 2) &#39;dir. Mekanik çekme eşiği mekanik elektronik bir dinamik taban von Frey aesthesiometer veya manuel von Frey kıllar 4 kullanarak yaralı ve yaralanmamış hindpaws hem de uyararak değerlendirilir. Mekanik bir geri çekme eşiği uygulanan yüksek basınç(Gram cinsinden) pençe çekme tetikler. İlk Hargreaves ve ark (1988) tarafından açıklanan ısıl çekmede gecikme, ölçülmesi için, sıçanın sol arka ayağını bir plantar analjezi metre 5,6 kullanarak şeffaf bir cam yüzeyi sayesinde mükemmel bir ısı ışınına maruz kalmaktadır. Isı uyarana çekilme gecikme yaralı ve yaralanmamış hindpaws hem pençe çekme için zaman olarak kaydedilir. Aşağıdaki CC, yaralı pençede mekanik çekme eşiği, hem de ısı geri çekme gecikmesi bazal ölçümler ve elin pençe (Şekil 3) ile karşılaştırıldığında, gerek önemli ölçüde azalır. Ağrı duyarlılık testi ile birlikte periferik sinir hasarı CCİ modeli kronik nöropatik ağrı değiştirmek için potansiyel terapötik ajanların etkinliğini araştırmak için bir model sistemi sağlar. Laboratuarımızda, biz patogenezinde nöro-immün etkileşimlerin rolünü araştırmak için hindpaws termal ve mekanik hassasiyet yanında CCİ kullanmaknöropatik ağrı ve tedavisi.

Watch this Scientific Journal Video about Chronic Constriction of the Sciatic Nerve and Pain Hypersensitivity Testing in Rats at JoVE.com

Chronic Constriction of the Sciatic Nerve and Pain Hypersensitivity Testing in Rats

Cerrahi basitlik ve sağlam davranışsal sonuçları nedeniyle, siyatik sinirin kronik daralma nöropatik ağrı seçkin hayvan modelleri biridir. Ameliyat sonrası 24 saat içinde, ağrı aşırı duyarlılık kurulan ve kantitatif bir von Frey aesthesiometer (mekanik test) ve plantar analjezi metre (termal testi) kullanılarak ölçülebilir.

Siyatik Sinir Sıçanlarda Ağrı aşırı duyarlılık Test Kronik daraltma

Chronic neuropathic pain, resulting from damage to the central or peripheral nervous system, is a prevalent and debilitating condition, affecting 7-18% of ...

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Medicine

Acute and Chronic Tactile Sensory Testing after Spinal Cord Injury in Rats

Sıçanlarda Spinal Kord Yaralanma sonrası Akut ve Kronik Dokunsal Duyu Testi

Spinal cord injury (SCI) impairs sensory systems causing allodynia1-8. To identify cellular and molecular causes of allodynia, sensitive and valid sensory ...

Ascending Aortic Constriction in Rats for Creation of Pressure Overload Cardiac Hypertrophy Model

Basınç Aşırı Kardiyak hipertrofi Modeli Oluşturma Ratlarda Aort daralma, Artan

Ascending aortic constriction is the most common and successful surgical model for creating pressure overload induced cardiac hypertrophy and heart ...

Urinary Bladder Distention Evoked Visceromotor Responses as a Model for Bladder Pain in Mice

Farelerde Mesane Ağrı için bir model olarak Mesane Distansiyonunun Uyarılmış Visceromotor Cevapları

Approximately 3-8 million people in the United States suffer from interstitial cystitis/bladder pain syndrome (IC/BPS), a debilitating condition ...

The Sciatic Nerve Cuffing Model of Neuropathic Pain in Mice

Farelerde Nöropatik Ağrı Siyatik Sinir cuffing Modeli

Neuropathic pain arises as a consequence of a lesion or a disease affecting the somatosensory system. This syndrome results from maladaptive changes in ...

Chronic Constriction Injury of the Rat's Infraorbital Nerve (IoN-CCI) to Study Trigeminal Neuropathic Pain

Chronic Constriction Injury of the Rat's Infraorbital Nerve IoN-CCI to Study Trigeminal Neuropathic Pain

Trigeminal Nöropatik Ağrı Eğitim için Rat adlı infraorbital Sinir (ION-CCI) kronik Daralma Yaralanma

Animal models are important tools to study the pathophysiology and pharmacology of neuropathic pain. This manuscript describes the surgical and behavioral ...

A Model of Cardiac Remodeling Through Constriction of the Abdominal Aorta in Rats

Sıçanlarda Karın Aort Konstrüksiyon sayesinde Kardiyak Yaptırma Bir Model

Heart failure is one of the leading causes of death worldwide. It is a complex clinical syndromethat includes fatigue, dyspnea, exercise intolerance, and ...

Histological Quantification of Chronic Myocardial Infarct in Rats

Sıçanlar Kronik Kalp Enfarktüsü histolojik Niceleme

Myocardial infarction is defined as cardiomyocyte death due to prolonged ischemia; an inflammatory response and scar formation (fibrosis) follow the ...

Minimally Invasive Transverse Aortic Constriction in Mice

Farelerde Minimal İnvaziv Enine Aort Konstrüksiyon

Minimally invasive transverse aortic constriction (MTAC) is a more desirable method for the constriction of the transverse aorta in mice than standard ...

Subcutaneous Trigeminal Nerve Field Stimulation for Refractory Facial Pain

Refrakter Yüz Ağrısı İçin Subkutan Trigeminal Sinir Sahası Stimülasyonu

Chronic or neuropathic trigeminal facial pain can be challenging to treat. Neurosurgical procedures should be applied when conservative treatment fails. ...

Development of Recombinant Proteins to Treat Chronic Pain

Kronik ağrı tedavisinde rekombinant proteinler gelişimi

Chronic pain is difficult to treat and new approaches to resolve persistent pain are urgently needed. Anti-inflammatory cytokines are promising candidates ...