Name: quantitative comparison of cis-regulatory element (cre) activities in transgenic drosophila melanogaster
Uploaded: 2011-12-19T12:00:00
Description: Watch this Scientific Journal Video about Quantitative Comparison of cis-Regulatory Element CRE Activities in Transgenic Drosophila melanogaster at JoVE.com

我们感谢：萨科Gompel和本雅明本议定书的发展所作出的贡献，以Prud&#39;homme梅利莎威廉姆斯和四个匿名评审专家对稿件的意见;研究奖学金战争的代顿大学研究生院和代顿大学生物学部和研究所（UDRI）研究TMW的支持。这项工作是由美国心脏协会授予11BGIA7280000 TMW的支持。

概述： 该视频演示了一个协议，能够定量测量果蝇（D.）果蝇的顺式调控元件（CRE）序列的基因调控活动。这个协议可以用来比较附体的监管活动：野生型和突变型的综合招聘考试形式，自然发生在一个物种，或分歧物种之间的同源CRES发现华润创业等位基因。 1。记者转基因定点整合到果蝇基因组答记者转基因建设<ol><li>?...

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顺式调控元件编码基因组计划，指定基因表达模式，从而发展的过程中，是两种基本形态演变2-4和5,6,19人类性状的表型变异的突变的显着位置。尽管在这方面的重要性，CRES监管逻辑仍然知之甚少。一个突出的原因，这种认识赤字一直缺乏合适的方法来定量比较CRES功能序列。在这里，我们提出了一个协议， 资本对果蝇添加一种定量方面的研究CRES改良的转基因方法。 ...

quantitative comparison of cis-regulatory element (cre) activities in transgenic drosophila melanogaster

基因表达模式指定的顺式调控元件（CRE）序列，也称为增强剂或顺式调控模块。一个典型的综合招聘考试拥有为几个转录因子的蛋白质，赋予监管的逻辑指定时，何地，在什么水平调控基因（S）表示结合位点的安排。一种动物基因组内的CRES的全套编码生物体的计划发展1和经验以及理论研究表明，在CRES突变发挥了突出的作用， 在形态进化2-4。此外，人类基因组范围关联研究表明，遗传变异，在CRES作出重大贡献的表型变异 5,6 。因此，了解监管逻辑和突变如何影响这样的逻辑，是遗传学的一个中心目标。 记者转基因提供了强大的的方法， 研究在体内功能TION的CRES。在这里，已知或可疑的综合招聘考试序列加上记者容易观察到的蛋白产物基因编码的异源启动子和编码序列。当记者转基因插入到宿主生物体，华润创业活动形式编码的记者蛋白质变成可见的。几十年来一直使用的P -元素介导转基因果蝇种果蝇（D.）果蝇 7引入这种模式生物的记者转基因，虽然转基因的基因组的位置是随机的。因此，报告基因的活性是由当地的染色质和基因环境的强烈影响，综合招聘考试比较限制被定性。近年来，phiC31集成系统被改编为在果蝇中使用转基因插入到特定的基因组的着陆点 8-10 。这种能力的基因的定量测量，以及与此有关，华润创业活动 11-13 FEasible。生产的转基因果蝇，可以外包，包括phiC31为基础的一体化，无需购买昂贵的设备和/或有能力在专门的转基因注入协议。 在这里，我们目前一般协议定量评价一个综合招聘考试的活动，并表明这种方法可以用来衡量一个综合招聘考试的活动引入突变的影响，并比较同源CRES活动。虽然给出的例子是为华润创业积极，在果蝇的变态，这种方法可以应用到其他发育阶段，果蝇物种，或模式生物。最终，一个更广泛地使用这种方法研究CRES应该提前了解监管逻辑和逻辑可以如何变化和演变。

Watch this Scientific Journal Video about Quantitative Comparison of cis-Regulatory Element CRE Activities in Transgenic Drosophila melanogaster at JoVE.com

Quantitative Comparison of cis-Regulatory Element CRE Activities in Transgenic Drosophila melanogaster

性状的表型变异可能导致的顺式调控元件（CRE）序列控制的基因表达模式的突变。在果蝇中使用派生的方法可以定量比较的空间和时间修改或自然发生的综合招聘考试的变体介导的基因表达模式的水平。

定量比较顺调节元件（CRE）的活动，在转基因果蝇</em

Gene expression patterns are specified by cis-regulatory element (CRE) sequences, which are also called enhancers or cis-regulatory modules. A typical CRE ...

Research

JoVE Journal

Biology

Quantitative Comparison of cis-Regulatory Element (CRE) Activities in Transgenic Drosophila melanogaster

Dissection of Larval CNS in Drosophila Melanogaster

在果蝇幼虫中枢神经系统的剖析

The central nervous system (CNS) of Drosophila larvae is complex and poorly understood.  One way to investigate the CNS is to use immunohistochemistry to ...

科研

生物学

High-Resolution Video Tracking of Locomotion in Adult Drosophila Melanogaster

高分辨率视频跟踪移动的，在成年果蝇。

Flies provide an important model for studying complex behavior due to the plethora of genetic tools available to researchers in this field.  Studying ...

Monitoring Heart Function in Larval Drosophila melanogaster for Physiological Studies

Monitoring Heart Function in Larval Drosophila melanogaster for Physiological Studies

在幼虫的监测心脏功能果蝇生理研究

We present various methods to record cardiac function in the larval Drosophila. The approaches allow heart rate to be measured in unrestrained  and  ...

Live Imaging Of Drosophila melanogaster Embryonic Hemocyte Migrations

Live Imaging Of Drosophila melanogaster Embryonic Hemocyte Migrations

实时成像果蝇胚胎血细胞迁移

Many studies address cell migration using in vitro methods, whereas the physiologically relevant environment is that of the organism itself. Here we ...

Dissection of Oenocytes from Adult Drosophila melanogaster

Dissection of Oenocytes from Adult Drosophila melanogaster

从成人Oenocytes剖析果蝇</em

In Drosophila melanogaster, as in other insects, a waxy layer on the outer surface of the cuticle, composed primarily of hydrocarbon compounds, provides ...

Isolation of Drosophila melanogaster Testes

Isolation of Drosophila melanogaster Testes

分离果蝇睾丸

The testes of Drosophila melanogaster provide an important model for the study of stem cell maintenance and differentiation, meiosis, and soma-germline ...

Measurement of Lifespan in Drosophila melanogaster

Measurement of Lifespan in Drosophila melanogaster

寿命测量<em&gt;果蝇</em

Aging is a phenomenon that results in steady physiological deterioration in nearly all organisms in which it has been examined, leading to reduced ...

Imaging Through the Pupal Case of Drosophila melanogaster

Imaging Through the Pupal Case of Drosophila melanogaster

通过影像学的蛹壳<em&gt;果蝇</em

The longstanding use of Drosophila as a model for cell and developmental biology has yielded an array of tools. Together, these techniques have enabled ...

Enhanced Northern Blot Detection of Small RNA Species in Drosophila Melanogaster

Enhanced Northern Blot Detection of Small RNA Species in Drosophila Melanogaster

增强Northern印迹检测小RNA种类的<em&gt;果蝇</em

The last decades have witnessed the explosion of scientific interest around gene expression control mechanisms at the RNA level. This branch of molecular ...

Maintenance of a Drosophila melanogaster Population Cage

Maintenance of a Drosophila melanogaster Population Cage

的维持<em&gt;果蝇</em&gt;人口笼

Large quantities of DNA, RNA, proteins and other cellular components are often required for biochemistry and molecular biology experiments. The short life ...