Name: mouse model of surgically-induced endometriosis by auto-transplantation of uterine tissue
Uploaded: 2012-01-06T12:00:00
Description: Watch this Scientific Journal Video about Mouse Model of Surgically-induced Endometriosis by Auto-transplantation of Uterine Tissue at JoVE.com

克里斯Kassotis这个手稿的严格审查和奥黛丽贝利和斯科特Korte博士，约瑟夫比曼，艾莉森Curfman，保罗甘，布里奇特Neibreggue，雅各Redel，艾米施罗德，迈亚斯坦伯格和Stacey Winkeler特别感谢他们在优化援助这种模式在我们的实验室。提供资助的临床Biodetectives培训资助（NIH T90的）（KEP），密苏里州的生命科学大学本科生研究机会计划研究理事会，穆，穆研究委员会的赠款和NIH R21HD056441（SCN）。

1。规划活畜手术<ol><li>确保相应的审批已收到与实验动物工作。 </li><li>订购小鼠，并允许至少一个星期驯化新的环境。 </li><li>雌性小鼠安置在接触到男性费洛蒙的情况下，可以停止循环，这种现象被称为惠滕效果15,16。为了保持骑自行车转移小鼠尿液浸泡过的男性床上用品的女性笼，每5天。另外，如果使用开顶式网箱，地方两个男性笼之间的女性笼保持经常骑自行车的女性。 </li><li>?...

<ol>
	<li>Simoens, S., Hummelshoj, L., D'Hooghe, T. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Endometriosis:+cost+estimates+and+methodological+perspective.">Endometriosis: cost estimates and methodological perspective.</a> Hum. Reprod. Update. 13, 395-404 (2007).</li><li>Grummer, R. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Animal+models+in+endometriosis+research.">Animal models in endometriosis research.</a> Hum. Reprod. Update. 12, 641-649 (2006).</li><li>Vernon, M. W., Wilson, E. A. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Studies+on+the+surgical+induction+of+endometriosis+in+the+rat.">Studies on the surgical induction of endometriosis in the rat.</a> Fertil. Steril. 44, 684-694 (1985).</li><li>Cummings, A. M., Metcalf, J. L. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Induction+of+endometriosis+in+mice:+a+new+model+sensitive+to+estrogen.">Induction of endometriosis in mice: a new model sensitive to estrogen.</a> Reprod. Toxicol. 9, 233-238 (1995).</li><li>Pelch, K. E. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Aberrant+gene+expression+profile+in+a+mouse+model+of+endometriosis+mirrors+that+observed+in+women.">Aberrant gene expression profile in a mouse model of endometriosis mirrors that observed in women.</a> Fertil. Steril. 93, 1615-1627 (2010).</li><li>Flores, I. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Molecular+profiling+of+experimental+endometriosis+identified+gene+expression+patterns+in+common+with+human+disease.">Molecular profiling of experimental endometriosis identified gene expression patterns in common with human disease.</a> Fertil. Steril. 87, 1180-1199 (2007).</li><li>Giudice, L. C., Kao, L. C. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Endometriosis.">Endometriosis.</a> Lancet. 364, 1789-1799 (2004).</li><li>Lin, Y. J., Lai, L. e. i., Y, H., Wing, L. Y. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Neutrophils+and+macrophages+promote+angiogenesis+in+the+early+stage+of+endometriosis+in+a+mouse+model.">Neutrophils and macrophages promote angiogenesis in the early stage of endometriosis in a mouse model.</a> Endocrinology. 147, 1278-1286 (2006).</li><li>Fang, Z. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Intact+progesterone+receptors+are+essential+to+counteract+the+proliferative+effect+of+estradiol+in+a+genetically+engineered+mouse+model+of+endometriosis.">Intact progesterone receptors are essential to counteract the proliferative effect of estradiol in a genetically engineered mouse model of endometriosis.</a> Fertil. Steril. 82, 673-678 (2004).</li><li>Fang, Z. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Genetic+or+enzymatic+disruption+of+aromatase+inhibits+the+growth+of+ectopic+uterine+tissue.">Genetic or enzymatic disruption of aromatase inhibits the growth of ectopic uterine tissue.</a> J. Clin. Endocrinol. Metab. 87, 3460-3466 (2002).</li><li>Cummings, A. M., Metcalf, J. L., Birnbaum, L. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Promotion+of+endometriosis+by+2,3,7,8-tetrachlorodibenzo-p-dioxin+in+rats+and+mice:+time-dose+dependence+and+species+comparison.">Promotion of endometriosis by 2,3,7,8-tetrachlorodibenzo-p-dioxin in rats and mice: time-dose dependence and species comparison.</a> Toxicol. Appl. Pharmacol. 138, 131-139 (1996).</li><li>Foster, W. G. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Morphologic+characteristics+of+endometriosis+in+the+mouse+model:+application+to+toxicology.">Morphologic characteristics of endometriosis in the mouse model: application to toxicology.</a> Can. J. Physiol. Pharmacol. 75, 1188-1196 (1997).</li><li>Cummings, A. M., Metcalf, J. L. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Effect+of+surgically+induced+endometriosis+on+pregnancy+and+effect+of+pregnancy+and+lactation+on+endometriosis+in+mice.">Effect of surgically induced endometriosis on pregnancy and effect of pregnancy and lactation on endometriosis in mice.</a> Proc. Soc. Exp. Biol. Med. 212, 332-337 (1996).</li><li>Lu, Y., Nie, J., Liu, X., Zheng, Y., Guo, S. W. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Trichostatin+A,+a+histone+deacetylase+inhibitor,+reduces+lesion+growth+and+hyperalgesia+in+experimentally+induced+endometriosis+in+mice.">Trichostatin A, a histone deacetylase inhibitor, reduces lesion growth and hyperalgesia in experimentally induced endometriosis in mice.</a> Hum. Reprod. 25, 1014-1025 (2010).</li><li>Whitten, W. K. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Modification+of+the+oestrous+cycle+of+the+mouse+by+external+stimuli+associated+with+the+male.">Modification of the oestrous cycle of the mouse by external stimuli associated with the male.</a> J. Endocrinol. 13, 399-404 (1956).</li><li>Whitten, W. K., Bronson, F. H., Greenstein, J. A. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Estrus-inducing+pheromone+of+male+mice:+transport+by+movement+of+air.">Estrus-inducing pheromone of male mice: transport by movement of air.</a> Science. 161, 584-585 (1968).</li><li>Goldman, J. M., Murr, A. S., Cooper, R. L. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=The+rodent+estrous+cycle:+characterization+of+vaginal+cytology+and+its+utility+in+toxicological+studies.">The rodent estrous cycle: characterization of vaginal cytology and its utility in toxicological studies.</a> Birth. Defects. Res. B. Dev. Reprod. Toxicol. 80, 84-97 (2007).</li><li>Pritchett-Corning, K. R., Mulder, G. B., Luo, Y., White, W. J. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Principles+of+Rodent+Surgery+for+the+New+Surgeon.">Principles of Rodent Surgery for the New Surgeon.</a> J. Vis. Exp. (47), e2586-e2586 (2011).</li><li>Moen, M. H., Halvorsen, T. B. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Histologic+confirmation+of+endometriosis+in+different+peritoneal+lesions.">Histologic confirmation of endometriosis in different peritoneal lesions.</a> Acta. Obstet. Gynecol. Scand. 71, 337-342 (1992).</li><li>Cummings, A. M. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Methoxychlor+as+a+model+for+environmental+estrogens.">Methoxychlor as a model for environmental estrogens.</a> Crit. Rev. Toxicol. 27, 367-379 (1997).</li><li>Fowler, R. E., Edwards, R. G. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Induction+of+superovulation+and+pregnancy+in+mature+mice+by+gonadotrophins.">Induction of superovulation and pregnancy in mature mice by gonadotrophins.</a> J. Endocrinol. 15, 374-384 (1957).</li><li>Wilson, E. D., Zarrow, M. X. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Comparison+of+superovulation+in+the+immature+mouse+and+rat.">Comparison of superovulation in the immature mouse and rat.</a> J. Reprod. Fertil. 3, 148-158 (1962).</li><li>Lee, B., Du, H., Taylor, H. S. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Experimental+murine+endometriosis+induces+DNA+methylation+and+altered+gene+expression+in+eutopic+endometrium.">Experimental murine endometriosis induces DNA methylation and altered gene expression in eutopic endometrium.</a> Biol. Reprod. 80, 79-85 (2009).</li><li>Somigliana, E. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Endometrial+ability+to+implant+in+ectopic+sites+can+be+prevented+by+interleukin-12+in+a+murine+model+of+endometriosis.">Endometrial ability to implant in ectopic sites can be prevented by interleukin-12 in a murine model of endometriosis.</a> Hum. Reprod. 14, 2944-2950 (1999).</li><li>Hirata, T. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Development+of+an+experimental+model+of+endometriosis+using+mice+that+ubiquitously+express+green+fluorescent+protein.">Development of an experimental model of endometriosis using mice that ubiquitously express green fluorescent protein.</a> Hum. Reprod. 20, 2092-2096 (2005).</li><li>Story, L., Kennedy, S. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Animal+studies+in+endometriosis:+a+review.">Animal studies in endometriosis: a review.</a> Ilar. J. 45, 132-138 (2004).</li><li>Cummings, A. M., Hedge, J. M., Birnbaum, L. S. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Effect+of+prenatal+exposure+to+TCDD+on+the+promotion+of+endometriotic+lesion+growth+by+TCDD+in+adult+female+rats+and+mice.">Effect of prenatal exposure to TCDD on the promotion of endometriotic lesion growth by TCDD in adult female rats and mice.</a> Toxicol. Sci. 52, 45-49 (1999).</li><li>Cummings, A. M., Metcalf, J. L. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Effects+of+estrogen,+progesterone,+and+methoxychlor+on+surgically+induced+endometriosis+in+rats.">Effects of estrogen, progesterone, and methoxychlor on surgically induced endometriosis in rats.</a> Fundam. Appl. Toxicol. 27, 287-290 (1995).</li><li>Sharpe-Timms, K. L. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Endometriotic+lesions+synthesize+and+secrete+a+haptoglobin-like+protein.">Endometriotic lesions synthesize and secrete a haptoglobin-like protein.</a> Biol. Reprod. 58, 988-994 (1998).</li><li>Yavuz, E., Oktem, M., Esinler, I., Toru, S. A., Zeyneloglu, H. B. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Genistein+causes+regression+of+endometriotic+implants+in+the+rat+model.">Genistein causes regression of endometriotic implants in the rat model.</a> Fertil. Steril. 88, 1129-1134 (2007).</li><li>Dmitrieva, N. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Endocannabinoid+involvement+in+endometriosis.">Endocannabinoid involvement in endometriosis.</a> Pain. 151, 703-710 (2010).</li><li>Efstathiou, J. A. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Nonsteroidal+antiinflammatory+drugs+differentially+suppress+endometriosis+in+a+murine+model.">Nonsteroidal antiinflammatory drugs differentially suppress endometriosis in a murine model.</a> Fertil. Steril. 83, 171-181 (2005).</li><li>Becker, C. M. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Endostatin+inhibits+the+growth+of+endometriotic+lesions+but+does+not+affect+fertility.">Endostatin inhibits the growth of endometriotic lesions but does not affect fertility.</a> Fertil. Steril. 84, 1144-1155 (2005).</li><li>Becker, C. M. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Short+synthetic+endostatin+peptides+inhibit+endothelial+migration+in+vitro+and+endometriosis+in+a+mouse+model.">Short synthetic endostatin peptides inhibit endothelial migration in vitro and endometriosis in a mouse model.</a> Fertil. Steril. 85, 71-77 (2006).</li><li>Sharpe-Timms, K. L. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Using+rats+as+a+research+model+for+the+study+of+endometriosis.">Using rats as a research model for the study of endometriosis.</a> Ann. N.Y. Acad. Sci. 955, 318-327 (2002).</li><li>Stilley, J. A., Woods-Marshall, R., Sutovsky, M., Sutovsky, P., Sharpe-Timms, K. L. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Reduced+Fecundity+in+Female+Rats+with+Surgically+Induced+Endometriosis+and+in+Their+Daughters:+A+Potential+Role+for+Tissue+Inhibitors+of+Metalloproteinase+1.">Reduced Fecundity in Female Rats with Surgically Induced Endometriosis and in Their Daughters: A Potential Role for Tissue Inhibitors of Metalloproteinase 1.</a> Biol. Reprod. 80, (2009).</li></ol>

有几个关键参数，应当指出，在执行子宫内膜异位症手术诱导小鼠。首先，子宫内膜异位症是一种雌激素依赖性疾病，应在完整的动物，或者在去卵巢动物辅以雌激素 20进行这种手术。二，缝合子宫内膜活检动脉级联必须格外小心。我们发现，只有两个有一个相对宽松的结抛出保持活检，同时防止结扎肠和随后的组织坏死和动物死亡的血液供应。我们强烈建议练上几个小鼠前实际实验，?...

<table border="1"><tbody><tr><td> 试剂/设备名称 </td><td> 公司 </td><td> 目录编号 </td></tr><tr><td>蜡笔</td><td>费舍尔</td><td> NC9954135 </td></tr><tr><td>载玻片</td><td>费舍尔</td><td> 12-550-433 </td></tr><tr><td>吸管</td><td>费舍尔</td><td> S79383 </td></tr><tr><td>阴道细胞学涂片评估标准光镜下</td><td></td><td></td></tr><tr><td>丁丙诺啡盐酸C3（CARJET）10X1ml </td><td>巴特勒动物保健供应</td><td> 022891 </td></tr><tr><td>无菌磷酸盐缓冲液（PBS） </td><td> Gibco公司</td><td> 14040-117 </td></tr><tr><td>万U / ml青霉素，10,000微克/毫升链霉素0.85％氯化钠</td><td> Hyclone公司</td><td> SV30010 </td></tr><tr><td>异氟醚</td><td>雅培动物健康</td><td> 05260-05 </td></tr><tr><td>非再吸入异氟醚麻醉系统</td><td></td><td></td></tr><tr><td>循环热水加热垫</td><td></td><td></td></tr><tr><td> 30毫升注射器鞘</td><td>费舍尔</td><td> 14 - 823 - 16G </td></tr><tr><td>无粉无菌手套</td><td> Fisherbrand </td><td> 19020558 </td></tr><tr><td>眼药膏</td><td>主要制药公司</td><td> 10033691 </td></tr><tr><td>小电器快船</td><td>华尔</td><td> 9861-600 </td></tr><tr><td>洗必泰擦洗</td><td>费舍尔</td><td> NC9863042 </td></tr><tr><td> 70％的乙醇</td><td></td><td></td></tr><tr><td> Polylined无菌场</td><td> Busse医院耗材</td><td> 696 </td></tr><tr><td>大小3手术刀</td><td>费舍尔</td><td> 22-079-657 </td></tr><tr><td> 10号手术刀片</td><td>费舍尔</td><td> 22-079-681 </td></tr><tr><td>小型手术剪</td><td> Roboz </td><td> RS - 5850 </td></tr><tr><td>小锯齿半弯钳</td><td> Roboz </td><td> RS - 5135 </td></tr><tr><td> 5-0黑色编织丝线缝合</td><td>爱惜康</td><td> K870H </td></tr><tr><td>消毒的耐热玻璃培养皿</td><td>康宁</td><td> 70160-101 </td></tr><tr><td> 2毫米活检冲床</td><td> Miltex </td><td> 33-31 </td></tr><tr><td>无菌纱布</td><td>肯德尔</td><td> 1806 </td></tr><tr><td> 6-0黑色单丝ethilon尼龙缝线</td><td>爱惜康</td><td> 697G </td></tr><tr><td>针驱动器（可选） </td><td>世界精密仪器</td><td> 500023 </td></tr><tr><td> 5-0染色编织涂vicryl缝合</td><td>爱惜康</td><td> J490G </td></tr><tr><td> 9毫米Autoclip伤口剪辑</td><td>碧迪</td><td> 427631 </td></tr><tr><td> Autoclip应用方及卸妆</td><td>碧迪</td><td> 427630 </td></tr><tr><td> 23G针</td><td>碧迪</td><td> 305193 </td></tr><tr><td> 1CC注射器</td><td>流式细胞迪克森</td><td> 301025 </td></tr><tr><td> 5倍的放大镜支架（可选） </td><td>费舍尔</td><td> 14-648-23 </td></tr><tr><td> 10％的福尔马林</td><td>费舍尔</td><td> SF100 - 4 </td></tr><tr><td>游标卡尺</td><td> Roboz </td><td> RS - 6466 </td></tr><tr><td>加工/嵌入录音带</td><td>费舍尔</td><td> 15 - 197 - 700A </td></tr><tr><td>活检泡沫垫</td><td>费舍尔</td><td> 22-038-222 </td></tr><tr><td> RNAqueous的RNA提取试剂盒</td><td>Ambion公司</td><td> AM1912 </td></tr><tr><td>液氮</td><td></td><td></td></tr><tr><td>捕捉第离心平顶管</td><td>费舍尔</td><td> 02-681-240 </td></tr><tr><td>氯胺酮（可选） </td><td> Simga </td><td> K4138 </td></tr><tr><td> Domitor（medetomidine盐酸盐）（可选） </td><td> Tocris </td><td> 2023 </td></tr><tr><td> Antisedan（atipamezole）（可选） </td><td>西格玛</td><td> A9611 </td></tr></tbody></table>

mouse model of surgically-induced endometriosis by auto-transplantation of uterine tissue

子宫内膜异位症是一种慢性，痛苦的疾病，其病因尚不清楚。此外，子宫内膜异位症的治疗，可以要求腹腔镜切除病灶和/或慢性疼痛和不孕症状的药品管理。已与子宫内膜异位症相关的成本估计为每年220亿美元，在美国的1 。为了进一步了解我们这个谜一般的疾病的基本机制的，动物模型已被采用。灵长类动物的自发发展子宫内膜异位症，因此灵长类动物模型最接近妇女疾病。然而，啮齿动物模型更具成本效益和随时可用2。我们在这里描述的模型，涉及到肠系膜（图1）自体子宫组织转移，并首次在大鼠 3，后来转移到鼠标的4 。手术引起的子宫内膜异位症的自体啮齿动物模型的目标是模仿在女性的疾病。我们和其他人曾在小鼠或大鼠镜观察到，在妇女疾病5,6子宫内膜异位病灶观察表明，改变基因的表达模式。鼠标在执行手术的优点之一是，大量的转基因小鼠品系可以帮助研究人员确定的具体组成部分子宫内膜异位症的建立和发展中的重要作用。另一种模式，即切除子宫内膜碎片是免疫功能低下小鼠的腹膜也被广泛使用，但受限于缺乏正常的免疫系统被认为是在子宫内膜异位症2,7的重要。重要的是，手术引起的子宫内膜异位症的小鼠模型是一个通用的模型已被用于研究免疫系统8，9,10和 11,12激素环境因素如何影响子宫内膜异位症，以及子宫内膜异位症的影响，对fertility 13和痛苦14。

Watch this Scientific Journal Video about Mouse Model of Surgically-induced Endometriosis by Auto-transplantation of Uterine Tissue at JoVE.com

Mouse Model of Surgically-induced Endometriosis by Auto-transplantation of Uterine Tissue

子宫内膜异位症手术诱导小鼠和大鼠子宫组织的自动移植到肠系膜动脉级联的描述。

自动子宫组织移植手术诱发子宫内膜异位症小鼠模型

Endometriosis is a chronic, painful disease whose etiology remains unknown. Furthermore, treatment of endometriosis can require laparoscopic removal of ...

Research

JoVE Journal

Medicine

Mouse Model of Intraluminal MCAO: Cerebral Infarct Evaluation by Cresyl Violet Staining

小鼠模型的腔内MCAO脑梗塞评价甲酚紫染色

Stroke is the third  cause of mortality and the leading cause of disability in the World. Ischemic  stroke accounts for approximately 80% of all strokes. ...

科研

医学

Implantation of Inferior Vena Cava Interposition Graft in Mouse Model

下腔静脉间置移植物在小鼠模型中植入

Biodegradable scaffolds seeded with bone marrow mononuclear cells (BMCs) are often used for reconstructive surgery to treat congenital cardiac anomalies. ...

Transplantation of Pulmonary Valve Using a Mouse Model of Heterotopic Heart Transplantation

肺动脉瓣使用异位心脏移植小鼠模型移植

Tissue engineered heart valves, especially decellularized valves, are starting to gain momentum in clinical use of reconstructive surgery with mixed ...

Rose Bengal Photothrombosis by Confocal Optical Imaging In Vivo: A Model of Single Vessel Stroke

Rose Bengal Photothrombosis by Confocal Optical Imaging In Vivo:  A Model of Single Vessel Stroke

玫瑰红Photothrombosis共聚焦光学成像<em&gt;在体内</em&gt;：单支脑卒中模型

In vivo imaging techniques have increased in utilization due to recent advances in imaging dyes and optical technologies, allowing for the ability to ...

Assessment of Cardiac Morphological and Functional Changes in Mouse Model of Transverse Aortic Constriction by Echocardiographic Imaging

在横向主动脉缩窄的小鼠模型和心脏形态学评估功能改变通过超声心动图成像

Transverse aortic constriction (TAC) in mice has been used as a valuable model to study mechanisms of cardiac hypertrophy and heart failure1. A reliable ...

Creation of a Rodent Model of Abdominal Aortic Aneurysm by Blocking Adventitial Vasa Vasorum Perfusion

阻断外瓦萨血管灌注对腹主动脉瘤鼠模型的建立

The adventitial vasa vasorum (VV) provides oxygen and nourishment to the aortic wall. Hypoxia in the aortic wall can cause enlarged abdominal aortic ...

Contractility Measurements of Human Uterine Smooth Muscle to Aid Drug Development

人子宫平滑肌的收缩力测量对药物开发的帮助

Discovery and characterization of novel pharmaceutical compounds or biochemical probes rely on robust and physiologically relevant assay systems. We ...

A Mouse Model of Orthopedic Surgery to Study Postoperative Cognitive Dysfunction and Tissue Regeneration

骨科手术的小鼠模型研究术后认知功能障碍与组织再生

Surgery is commonly used to improve and maintain quality of life. Unfortunately, in vulnerable patients such as the elderly, complications may occur and ...

Improvement of a Closed Chest Porcine Myocardial Infarction Model by Standardization of Tissue and Blood Sampling Procedures

组织和采血程序标准化改善闭合性胸猪心肌梗死模型的研究

Myocardial ischemia reperfusion (I/R) injury contributes to almost half of the necrotic area after myocardial infarction. To date there is no approved ...

Isometric Contractility Measurement of the Mouse Mesenteric Artery Using Wire Myography

用线 Myography 测定小鼠肠系膜动脉的等轴收缩力

The wire myograph technique is used to assess the contractility of vascular smooth muscles in response to depolarization, GPCR agonists/inhibitors and ...