Name: mouse model of surgically-induced endometriosis by auto-transplantation of uterine tissue
Uploaded: 2012-01-06T12:00:00
Description: Watch this Scientific Journal Video about Mouse Model of Surgically-induced Endometriosis by Auto-transplantation of Uterine Tissue at JoVE.com

Særlig tak til Chris Kassotis og Audrey Bailey til kritisk revision af dette manuskript og til Dr. Scott Korte, Joseph Beeman, Alison Curfman, Paul Kimball, Bridget Neibreggue, Jacob Redel, Amy Schroder, Maija Steinberg, og Stacey Winkeler for deres assistance i optimering af denne model i vores laboratorium. Finansieringen kom fra Klinisk Biodetectives Training Grant (NIH T90) (KEP), University of Missouri Life Sciences Undergraduate Research Muligheder Program, MU Forskningsråd, MU Research Board tilskud og NIH R21HD056441 (SCN).

1. Planlægning af levende dyr operation <ol><li> Sikre, at relevante godkendelse er modtaget for at arbejde med forsøgsdyr. </li><li> Bestil mus og giver en frist på mindst en uge af Akklimatisering til deres nye omgivelser. </li><li> Hunmus til huse i mangel af eksponeringen for mandlige feromoner kan stoppe cykling, et fænomen kaldet Whitten effekt 15,16. For at holde mus cykling transfer urin-gennemblødt mandlige sengetøj til den kvindelige buret hver fem dage. Alternativt, hvis open-top-bure skal ...

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E., Edwards, R. G. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Induction+of+superovulation+and+pregnancy+in+mature+mice+by+gonadotrophins.">Induction of superovulation and pregnancy in mature mice by gonadotrophins.</a> J. Endocrinol. 15, 374-384 (1957).</li><li>Wilson, E. D., Zarrow, M. X. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Comparison+of+superovulation+in+the+immature+mouse+and+rat.">Comparison of superovulation in the immature mouse and rat.</a> J. Reprod. Fertil. 3, 148-158 (1962).</li><li>Lee, B., Du, H., Taylor, H. S. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Experimental+murine+endometriosis+induces+DNA+methylation+and+altered+gene+expression+in+eutopic+endometrium.">Experimental murine endometriosis induces DNA methylation and altered gene expression in eutopic endometrium.</a> Biol. Reprod. 80, 79-85 (2009).</li><li>Somigliana, E. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Endometrial+ability+to+implant+in+ectopic+sites+can+be+prevented+by+interleukin-12+in+a+murine+model+of+endometriosis.">Endometrial ability to implant in ectopic sites can be prevented by interleukin-12 in a murine model of endometriosis.</a> Hum. Reprod. 14, 2944-2950 (1999).</li><li>Hirata, T. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Development+of+an+experimental+model+of+endometriosis+using+mice+that+ubiquitously+express+green+fluorescent+protein.">Development of an experimental model of endometriosis using mice that ubiquitously express green fluorescent protein.</a> Hum. Reprod. 20, 2092-2096 (2005).</li><li>Story, L., Kennedy, S. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Animal+studies+in+endometriosis:+a+review.">Animal studies in endometriosis: a review.</a> Ilar. J. 45, 132-138 (2004).</li><li>Cummings, A. M., Hedge, J. M., Birnbaum, L. S. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Effect+of+prenatal+exposure+to+TCDD+on+the+promotion+of+endometriotic+lesion+growth+by+TCDD+in+adult+female+rats+and+mice.">Effect of prenatal exposure to TCDD on the promotion of endometriotic lesion growth by TCDD in adult female rats and mice.</a> Toxicol. Sci. 52, 45-49 (1999).</li><li>Cummings, A. M., Metcalf, J. L. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Effects+of+estrogen,+progesterone,+and+methoxychlor+on+surgically+induced+endometriosis+in+rats.">Effects of estrogen, progesterone, and methoxychlor on surgically induced endometriosis in rats.</a> Fundam. Appl. Toxicol. 27, 287-290 (1995).</li><li>Sharpe-Timms, K. L. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Endometriotic+lesions+synthesize+and+secrete+a+haptoglobin-like+protein.">Endometriotic lesions synthesize and secrete a haptoglobin-like protein.</a> Biol. Reprod. 58, 988-994 (1998).</li><li>Yavuz, E., Oktem, M., Esinler, I., Toru, S. A., Zeyneloglu, H. B. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Genistein+causes+regression+of+endometriotic+implants+in+the+rat+model.">Genistein causes regression of endometriotic implants in the rat model.</a> Fertil. Steril. 88, 1129-1134 (2007).</li><li>Dmitrieva, N. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Endocannabinoid+involvement+in+endometriosis.">Endocannabinoid involvement in endometriosis.</a> Pain. 151, 703-710 (2010).</li><li>Efstathiou, J. A. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Nonsteroidal+antiinflammatory+drugs+differentially+suppress+endometriosis+in+a+murine+model.">Nonsteroidal antiinflammatory drugs differentially suppress endometriosis in a murine model.</a> Fertil. Steril. 83, 171-181 (2005).</li><li>Becker, C. M. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Endostatin+inhibits+the+growth+of+endometriotic+lesions+but+does+not+affect+fertility.">Endostatin inhibits the growth of endometriotic lesions but does not affect fertility.</a> Fertil. Steril. 84, 1144-1155 (2005).</li><li>Becker, C. M. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Short+synthetic+endostatin+peptides+inhibit+endothelial+migration+in+vitro+and+endometriosis+in+a+mouse+model.">Short synthetic endostatin peptides inhibit endothelial migration in vitro and endometriosis in a mouse model.</a> Fertil. Steril. 85, 71-77 (2006).</li><li>Sharpe-Timms, K. 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Der er flere kritiske parametre, der bør bemærkes, mens de udfører de kirurgiske induktion af endometriose hos mus. Først endometriose er en østrogenafhængige sygdom, og som sådan denne operation skal udføres i intakte dyr eller alternativt i ovariectomized dyr suppleret med østrogener 20. For det andet skal suturering endometrie biopsier til arterielle kaskade udføres med største omhu. Vi har konstateret, at med kun to forholdsvis løs knob med én smide hver holder biopsi på plads, og samtidig f...

<table border="1"><tbody><tr><td> Navn på reagenset / udstyr </td><td> Firma </td><td> Katalog nummer </td></tr><tr><td> Voks blyant </td><td> Fisher </td><td> NC9954135 </td></tr><tr><td> Objektglas </td><td> Fisher </td><td> 12-550-433 </td></tr><tr><td> Pipette </td><td> Fisher </td><td> S79383 </td></tr><tr><td> Standard lysmikroskop for at vurdere, vaginal cytologi udtværet </td><td></td><td></td></tr><tr><td> Buprenorphin HCL C3 (CARJET) 10X1ml </td><td> Butler Dyresundhed Supply </td><td> 022891 </td></tr><tr><td> Sterile fosfatbufferet saltopløsning (PBS) </td><td> Gibco </td><td> 14040-117 </td></tr><tr><td> 10.000 E / ml penicillin, 10.000 mg / ml Streptomycin i 0,85% NaCl </td><td> Hyclone </td><td> SV30010 </td></tr><tr><td> Isofluran </td><td> AbbottAnimal Health </td><td> 05260-05 </td></tr><tr><td> Isofluran ikke-genåndingssystem anæstesisystemet </td><td></td><td></td></tr><tr><td> Recirkulerende varmt vand varmepude </td><td></td><td></td></tr><tr><td> 30 ml sprøjte kappe </td><td> Fisher </td><td> 14 til 823-16G </td></tr><tr><td> Pulver gratis sterile handsker </td><td> Fisherbrand </td><td> 19020558 </td></tr><tr><td> Oftalmologiske salve </td><td> Major Pharmaceuticals </td><td> 10033691 </td></tr><tr><td> Små elektriske klippere </td><td> Wahl </td><td> 9861-600 </td></tr><tr><td> Klorhexidin krat </td><td> Fisher </td><td> NC9863042 </td></tr><tr><td> 70% ethanol </td><td></td><td></td></tr><tr><td> Polylined sterile felt </td><td> Busse Hospital Engangsartikler </td><td> 696 </td></tr><tr><td> Størrelse 3 skalpel </td><td> Fisher </td><td> 22-079-657 </td></tr><tr><td> Number 10 skalpel knive </td><td> Fisher </td><td> 22-079-681 </td></tr><tr><td> Små kirurgiske sakse </td><td> Roboz </td><td> RS-5850 </td></tr><tr><td> Små savtakket semi-kurvede pincet </td><td> Roboz </td><td> RS-5135 </td></tr><tr><td> 5-0 sort flettet silke sutur </td><td> Ethicon </td><td> K870H </td></tr><tr><td> Steriliseret pyrexglas Petriskålene </td><td> Corning </td><td> 70160-101 </td></tr><tr><td> 2 mm biopsi Punch </td><td> Miltex </td><td> 33-31 </td></tr><tr><td> Sterile gaze </td><td> Kendall </td><td> 1806 </td></tr><tr><td> 6-0 sorte monofilamenter ethilon nylon sutur </td><td> Ethicon </td><td> 697G </td></tr><tr><td> Needle drivere (valgfrit) </td><td> Verdens præcisionsinstrumenter </td><td> 500023 </td></tr><tr><td> 5-0 ufarvede flettet belagt vicryl sutur </td><td> Ethicon </td><td> J490G </td></tr><tr><td> 9mm Autoclip såret klip </td><td> Becton Dickinson </td><td> 427631 </td></tr><tr><td> Autoclip applier &amp; remover </td><td> Becton Dickinson </td><td> 427630 </td></tr><tr><td> 23G kanyle </td><td> Becton Dickinson </td><td> 305193 </td></tr><tr><td> 1ml sprøjte </td><td> Becton Dickson </td><td> 301025 </td></tr><tr><td> 5X forstørrelsesglas stativ (ekstraudstyr) </td><td> Fisher </td><td> 14-648-23 </td></tr><tr><td> 10% bufferet formalin </td><td> Fisher </td><td> SF100-4 </td></tr><tr><td> Calipre </td><td> Roboz </td><td> RS-6466 </td></tr><tr><td> Behandling / embedding kassetter </td><td> Fisher </td><td> 15 til 197-700A </td></tr><tr><td> Biopsi skum puder </td><td> Fisher </td><td> 22-038-222 </td></tr><tr><td> RNAqueous RNA isolation kit </td><td>Ambion </td><td> AM1912 </td></tr><tr><td> Flydende kvælstof </td><td></td><td></td></tr><tr><td> Snap cap mikrocentrifuge fladt overrør </td><td> Fisher </td><td> 02-681-240 </td></tr><tr><td> Ketamin (valgfrit) </td><td> Simga </td><td> K4138 </td></tr><tr><td> Domitor (medetomidinhydrochlorid) (valgfrit) </td><td> Tocris </td><td> 2023 </td></tr><tr><td> Antisedan (atipamezol) (valgfrit) </td><td> Sigma </td><td> A9611 </td></tr></tbody></table>

mouse model of surgically-induced endometriosis by auto-transplantation of uterine tissue

Endometriose er en kronisk, smertefuld sygdom, hvis ætiologi er fortsat ukendt. Desuden kan behandlingen af ​​endometriose kræver laparoskopisk fjernelse af læsioner, og / eller kroniske farmaceutiske behandling af smerter og infertilitet symptomer. Omkostningerne forbundet med endometriose er blevet anslået til 22 milliarder dollars om året i USA 1. For yderligere at vores forståelse af mekanismerne bag denne gådefulde sygdom, har dyremodeller været ansat. Primater spontant udvikler endometriose og derfor primater modeller nærmest ligner sygdommen hos kvinder. Gnaver modeller, er dog mere omkostningseffektive og let tilgængelige 2. Den model, som vi beskriver her, indebærer en autolog overførsel af uterin væv til tarmenes mesenterium (Figur 1) og blev første gang udviklet i rotter 3 og senere overført til musen 4. Målet med autologe gnaver model af kirurgisk induceret endometriose er at efterlignesygdommen hos kvinder. Vi og andre har tidligere vist, at den ændrede genekspression mønster observeret i endometriotic læsioner fra mus eller rotter, spejle, der blev observeret hos kvinder med sygdommen 5,6. En fordel ved at udføre operationen i musen er, at den overflod af transgene mus stammer ledigt. kan støtte forskere til at bestemme betydningen af ​​specifikke komponenter vigtig i etablering og vækst af endometriose. En alternativ model, hvor fjernet menneskelige endometrie fragmenter bliver introduceret til bughinden af immunkompromitterede mus er også meget anvendt, men er begrænset af manglen på et normalt immunforsvar, som menes at være vigtigt i endometriose 2,7. Vigtigere er det, musemodel af kirurgisk induceret endometriose er en alsidig model, der er blevet brugt til at studere, hvordan immunsystemet 8, hormoner 9,10 og miljømæssige faktorer 11,12 påvirker endometriose samt virkningerne af endometriose på Fertility 13 og smerte 14.

Watch this Scientific Journal Video about Mouse Model of Surgically-induced Endometriosis by Auto-transplantation of Uterine Tissue at JoVE.com

Mouse Model of Surgically-induced Endometriosis by Auto-transplantation of Uterine Tissue

En beskrivelse af den kirurgiske induktion af endometriose hos mus og rotter ved auto-transplantation af uterine væv til arterielle kaskade af den intestinale mesenterium.

Musemodel af kirurgisk induceret Endometriose af Auto-transplantation af uterin væv

Endometriosis is a chronic, painful disease whose etiology remains unknown. Furthermore, treatment of endometriosis can require laparoscopic removal of ...

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Implantation af ringere Vena Cava indskydning Graft i musemodel

Biodegradable scaffolds seeded with bone marrow mononuclear cells (BMCs) are often used for reconstructive surgery to treat congenital cardiac anomalies. ...

Transplantation of Pulmonary Valve Using a Mouse Model of Heterotopic Heart Transplantation

Transplantation af pulmonale ventilen med en musemodel af Heterotop Heart Transplantation

Tissue engineered heart valves, especially decellularized valves, are starting to gain momentum in clinical use of reconstructive surgery with mixed ...

Rose Bengal Photothrombosis by Confocal Optical Imaging In Vivo: A Model of Single Vessel Stroke

Rose Bengal Photothrombosis by Confocal Optical Imaging In Vivo:  A Model of Single Vessel Stroke

Rose Bengal Photothrombosis ved Konfokal Optical Imaging<em&gt; In vivo</em&gt;: En model af Single Vessel Stroke

In vivo imaging techniques have increased in utilization due to recent advances in imaging dyes and optical technologies, allowing for the ability to ...

Assessment of Cardiac Morphological and Functional Changes in Mouse Model of Transverse Aortic Constriction by Echocardiographic Imaging

Vurdering af Cardiac Morfologisk og funktionelle ændringer i musemodel for Tværgående pulsåresammensnøring ved ekkokardiografisk Imaging

Transverse aortic constriction (TAC) in mice has been used as a valuable model to study mechanisms of cardiac hypertrophy and heart failure1. A reliable ...

Creation of a Rodent Model of Abdominal Aortic Aneurysm by Blocking Adventitial Vasa Vasorum Perfusion

Oprettelsen af en gnaver Model af abdominale aorta aneurisme ved at blokere Adventitial Vasa Vasorum Perfusion

The adventitial vasa vasorum (VV) provides oxygen and nourishment to the aortic wall. Hypoxia in the aortic wall can cause enlarged abdominal aortic ...

Contractility Measurements of Human Uterine Smooth Muscle to Aid Drug Development

Kontraktilitet målinger af menneskelige livmoder glatte muskelceller til at støtte udvikling af lægemidler

Discovery and characterization of novel pharmaceutical compounds or biochemical probes rely on robust and physiologically relevant assay systems. We ...

A Mouse Model of Orthopedic Surgery to Study Postoperative Cognitive Dysfunction and Tissue Regeneration

En musemodel af ortopædisk kirurgi til at studere postoperativ kognitiv dysfunktion og vævsregeneration

Surgery is commonly used to improve and maintain quality of life. Unfortunately, in vulnerable patients such as the elderly, complications may occur and ...

Improvement of a Closed Chest Porcine Myocardial Infarction Model by Standardization of Tissue and Blood Sampling Procedures

Forbedring af en lukket brystet svin myokardieinfarkt Model af standardisering af væv og blod prøveudtagningsprocedurer

Myocardial ischemia reperfusion (I/R) injury contributes to almost half of the necrotic area after myocardial infarction. To date there is no approved ...

Isometric Contractility Measurement of the Mouse Mesenteric Artery Using Wire Myography

Isometrisk kontraktilitet måling af musen mesenterica arterie ved hjælp af Wire blodkar

The wire myograph technique is used to assess the contractility of vascular smooth muscles in response to depolarization, GPCR agonists/inhibitors and ...