Name: mouse model of surgically-induced endometriosis by auto-transplantation of uterine tissue
Uploaded: 2012-01-06T12:00:00
Description: Watch this Scientific Journal Video about Mouse Model of Surgically-induced Endometriosis by Auto-transplantation of Uterine Tissue at JoVE.com

Speciale dank aan Chris Kassotis en Audrey Bailey voor een kritische herziening van dit manuscript en aan Dr Scott Korte, Joseph Beeman, Alison Curfman, Paul Kimball, Bridget Neibreggue, Jacob Redel, Amy Schroder, Maija Steinberg, en Stacey Winkeler voor hun hulp bij optimalisatie van dit model in ons laboratorium. De financiering werd verstrekt door de Clinical Biodetectives Training Grant (NIH T90) (KEP), Universiteit van Missouri Life Sciences Undergraduate Research Opportunities Program, MU Research Council, MU Research Board subsidies en NIH R21HD056441 (SCN).

1. Planning voor levende dieren chirurgie <ol><li> Zorg ervoor dat er passende goedkeuring is ontvangen om te werken met proefdieren. </li><li> Om muizen en laat minstens een week van acclimatisatie aan hun nieuwe omgeving. </li><li> Vrouwelijke muizen gehuisvest in de afwezigheid van blootstelling aan mannelijke feromonen kunnen stoppen met fietsen, een fenomeen als het Whitten effect 15,16 genoemd. Om muizen fiets overdracht urine doordrenkte mannen beddengoed aan de vrouwelijke kooi om de vijf dagen. Als...

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E., Edwards, R. G. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Induction+of+superovulation+and+pregnancy+in+mature+mice+by+gonadotrophins.">Induction of superovulation and pregnancy in mature mice by gonadotrophins.</a> J. Endocrinol. 15, 374-384 (1957).</li><li>Wilson, E. D., Zarrow, M. X. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Comparison+of+superovulation+in+the+immature+mouse+and+rat.">Comparison of superovulation in the immature mouse and rat.</a> J. Reprod. Fertil. 3, 148-158 (1962).</li><li>Lee, B., Du, H., Taylor, H. S. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Experimental+murine+endometriosis+induces+DNA+methylation+and+altered+gene+expression+in+eutopic+endometrium.">Experimental murine endometriosis induces DNA methylation and altered gene expression in eutopic endometrium.</a> Biol. Reprod. 80, 79-85 (2009).</li><li>Somigliana, E. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Endometrial+ability+to+implant+in+ectopic+sites+can+be+prevented+by+interleukin-12+in+a+murine+model+of+endometriosis.">Endometrial ability to implant in ectopic sites can be prevented by interleukin-12 in a murine model of endometriosis.</a> Hum. 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Er zijn een aantal kritische parameters die moet worden opgemerkt tijdens het uitvoeren van de chirurgische inductie van endometriose bij muizen. De eerste, endometriose is een oestrogeengevoelige ziekte en als zodanig deze operatie moeten worden uitgevoerd in intacte dieren of als alternatief in ovariëctomie dieren aangevuld met oestrogenen 20. Ten tweede moet hechten de endometriale biopsies om de arteriële cascade worden uitgevoerd met uiterste zorg. We hebben gevonden dat het gebruik van slechts twee re...

<table border="1"><tbody><tr><td> Naam van het reagens / apparatuur </td><td> Vennootschap </td><td> Catalogus nummer </td></tr><tr><td> Wax potlood </td><td> Visser </td><td> NC9954135 </td></tr><tr><td> Glasplaatje </td><td> Visser </td><td> 12-550-433 </td></tr><tr><td> Oogdruppelaar </td><td> Visser </td><td> S79383 </td></tr><tr><td> Standaard lichtmicroscoop voor het evalueren van vaginale cytologie uitstrijkjes </td><td></td><td></td></tr><tr><td> Buprenorfine HCL c3 (CARJET) 10X1ml </td><td> Butler Animal Health Supply </td><td> 022891 </td></tr><tr><td> Steriele fosfaat gebufferde zoutoplossing (PBS) </td><td> Gibco </td><td> 14040-117 </td></tr><tr><td> 10000 U / ml penicilline, 10.000 ug / ml streptomycine in 0,85% NaCl </td><td> Hyclone </td><td> SV30010 </td></tr><tr><td> Isofluraan </td><td> AbbottAnimal Health </td><td> 05260-05 </td></tr><tr><td> Isofluraan non-rebreathing verdoving systeem </td><td></td><td></td></tr><tr><td> Recirculerende warm water verwarming pad </td><td></td><td></td></tr><tr><td> 30 ml spuit schede </td><td> Visser </td><td> 14 tot 823-16G </td></tr><tr><td> Poeder vrij steriele handschoenen </td><td> Fisherbrand </td><td> 19020558 </td></tr><tr><td> Oogzalf </td><td> Grote Pharmaceuticals </td><td> 10033691 </td></tr><tr><td> Kleine elektrische tondeuse </td><td> Wahl </td><td> 9861-600 </td></tr><tr><td> Chloorhexidine scrub </td><td> Visser </td><td> NC9863042 </td></tr><tr><td> 70% Ethanol </td><td></td><td></td></tr><tr><td> Polylined steriel veld </td><td> Busse Ziekenhuis Disposables </td><td> 696 </td></tr><tr><td> Maat 3 scalpel </td><td> Visser </td><td> 22-079-657 </td></tr><tr><td> Nummer 10 scalpelmesjes </td><td> Visser </td><td> 22-079-681 </td></tr><tr><td> Kleine chirurgische schaar </td><td> Roboz </td><td> RS-5850 </td></tr><tr><td> Kleine getande semi-gebogen pincet </td><td> Roboz </td><td> RS-5135 </td></tr><tr><td> 5-0 zwart gevlochten zijden hechtdraad </td><td> Ethicon </td><td> K870H </td></tr><tr><td> Gesteriliseerd Pyrex-glazen petrischalen </td><td> Corning </td><td> 70160-101 </td></tr><tr><td> 2 mm biopsie punch </td><td> Miltex </td><td> 33-31 </td></tr><tr><td> Steriel gaas </td><td> Kendall </td><td> 1806 </td></tr><tr><td> 6-0 zwart monofilament ethilon nylon hechtdraad </td><td> Ethicon </td><td> 697G </td></tr><tr><td> Naald drivers (optioneel) </td><td> Wereld precisie-instrumenten </td><td> 500023 </td></tr><tr><td> 5-0 ongeverfde gevlochten gecoat vicryl hechtdraad </td><td> Ethicon </td><td> J490G </td></tr><tr><td> 9mm Autoclip wond clips </td><td> Becton Dickinson </td><td> 427631 </td></tr><tr><td> Autoclip aanvrager &amp; remover </td><td> Becton Dickinson </td><td> 427630 </td></tr><tr><td> 23G naald </td><td> Becton Dickinson </td><td> 305193 </td></tr><tr><td> 1cc spuit </td><td> Becton Dickson </td><td> 301025 </td></tr><tr><td> 5X vergrootglas (optioneel) </td><td> Visser </td><td> 14-648-23 </td></tr><tr><td> 10% gebufferde formaline </td><td> Visser </td><td> SF100-4 </td></tr><tr><td> Calipers </td><td> Roboz </td><td> RS-6466 </td></tr><tr><td> Verwerking / embedding cassettes </td><td> Visser </td><td> 15 tot 197-700A </td></tr><tr><td> Biopsie foam pads </td><td> Visser </td><td> 22-038-222 </td></tr><tr><td> RNAqueous RNA isolatie kit </td><td>Ambion </td><td> AM1912 </td></tr><tr><td> Vloeibare stikstof </td><td></td><td></td></tr><tr><td> Snap cap microcentrifugebuis vlakke bovenbuis </td><td> Visser </td><td> 02-681-240 </td></tr><tr><td> Ketamine (optioneel) </td><td> Simga </td><td> K4138 </td></tr><tr><td> Domitor (medetomidine hydrochloride) (optioneel) </td><td> Tocris </td><td> 2023 </td></tr><tr><td> Antisedan (atipamezol) (optioneel) </td><td> Sigma </td><td> A9611 </td></tr></tbody></table>

mouse model of surgically-induced endometriosis by auto-transplantation of uterine tissue

Endometriose is een chronische, pijnlijke ziekte waarvan de oorzaak blijft onbekend. Bovendien kan behandeling van endometriose moet laparoscopische verwijdering van de laesies, en / of chronische farmaceutische het beheer van de pijn en onvruchtbaarheid symptomen. De kosten verbonden aan endometriose wordt geschat op 22 miljard dollar per jaar in de Verenigde Staten 1. Om verdere ons begrip van de mechanismen die ten grondslag liggen aan deze raadselachtige ziekte, zijn diermodellen in dienst. Primaten spontaan ontwikkelen endometriose en daarom primaatmodellen het meest lijken op de ziekte bij vrouwen. Knaagdier modellen zijn echter meer kosteneffectieve en gemakkelijk beschikbaar 2. Het model dat we hier beschrijven betreft een autologe overdracht van de baarmoeder weefsel van de darm mesenterium (figuur 1) en werd voor het eerst ontwikkeld in de rat 3 en later overgebracht naar de muis 4. Het doel van de autologe knaagdier model van chirurgisch geïnduceerde endometriose is na te bootsen omde ziekte bij vrouwen. Wij en anderen hebben eerder aangetoond dat de veranderde genexpressie patroon dat in endometriose letsels van muizen of ratten spiegels die bij vrouwen waargenomen met de ziekte 5,6. Een voordeel van het uitvoeren van de operatie in de muis is dat de overvloed van transgene muizenstammen beschikbaar kan helpen onderzoekers bij het bepalen van de rol van specifieke componenten van belang bij de oprichting en groei van endometriose. Een alternatief model waarin weggesneden menselijke endometrium fragmenten maken kennis met het buikvlies van muizen immuun wordt ook veel gebruikt, maar wordt beperkt door het ontbreken van een normale immuunsysteem dat wordt gedacht dat belangrijk zijn in endometriose 2,7. Belangrijk is dat de muis model van chirurgisch geïnduceerde endometriose is een veelzijdig model dat is gebruikt om te bestuderen hoe het afweersysteem 8, hormonen 9,10 en omgevingsfactoren 11,12 endometriose, alsmede de gevolgen van endometriose op de Fert invloedafb reekbaarheid 13 en pijn 14.

Watch this Scientific Journal Video about Mouse Model of Surgically-induced Endometriosis by Auto-transplantation of Uterine Tissue at JoVE.com

Mouse Model of Surgically-induced Endometriosis by Auto-transplantation of Uterine Tissue

Een beschrijving van de chirurgische inductie van endometriose bij muizen en ratten door auto-transplantatie van baarmoeder weefsel om de arteriële cascade van de intestinale mesenterium.

Muismodel van chirurgisch geïnduceerde Endometriose door Auto-transplantatie van baarmoeder weefsel

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Muis Model van Intraluminale mCaO: herseninfarct Evaluatie door cresyl Violet Staining

Stroke is the third  cause of mortality and the leading cause of disability in the World. Ischemic  stroke accounts for approximately 80% of all strokes. ...

Implantation of Inferior Vena Cava Interposition Graft in Mouse Model

Implantatie van de vena cava inferior Interpositie Graft in muismodel

Biodegradable scaffolds seeded with bone marrow mononuclear cells (BMCs) are often used for reconstructive surgery to treat congenital cardiac anomalies. ...

Transplantation of Pulmonary Valve Using a Mouse Model of Heterotopic Heart Transplantation

Transplantatie van Pulmonary Valve een muis model van Heterotope Heart Transplantation

Tissue engineered heart valves, especially decellularized valves, are starting to gain momentum in clinical use of reconstructive surgery with mixed ...

Rose Bengal Photothrombosis by Confocal Optical Imaging In Vivo: A Model of Single Vessel Stroke

Rose Bengal Photothrombosis by Confocal Optical Imaging In Vivo:  A Model of Single Vessel Stroke

Rose Bengal Photothrombosis door confocale Optical Imaging<em&gt; In Vivo</em&gt;: Een model van de Single Vessel Stroke

In vivo imaging techniques have increased in utilization due to recent advances in imaging dyes and optical technologies, allowing for the ability to ...

Assessment of Cardiac Morphological and Functional Changes in Mouse Model of Transverse Aortic Constriction by Echocardiographic Imaging

Beoordeling van Cardiac morfologische en functionele veranderingen in muismodel van Transversale Aorta Vernauwing door Echocardiografie Imaging

Transverse aortic constriction (TAC) in mice has been used as a valuable model to study mechanisms of cardiac hypertrophy and heart failure1. A reliable ...

Creation of a Rodent Model of Abdominal Aortic Aneurysm by Blocking Adventitial Vasa Vasorum Perfusion

Oprichting van een knaagdier Model van abdominale aorta aneurysma door het blokkeren van Adventitial Vasa Vasorum perfusie

The adventitial vasa vasorum (VV) provides oxygen and nourishment to the aortic wall. Hypoxia in the aortic wall can cause enlarged abdominal aortic ...

Contractility Measurements of Human Uterine Smooth Muscle to Aid Drug Development

Contractility metingen van menselijke uteriene gladde spieren aan steun Geneesmiddelenontwikkeling

Discovery and characterization of novel pharmaceutical compounds or biochemical probes rely on robust and physiologically relevant assay systems. We ...

A Mouse Model of Orthopedic Surgery to Study Postoperative Cognitive Dysfunction and Tissue Regeneration

Een muismodel van orthopedische chirurgie te bestuderen Weefselregeneratie en postoperatieve cognitieve dysfunctie

Surgery is commonly used to improve and maintain quality of life. Unfortunately, in vulnerable patients such as the elderly, complications may occur and ...

Improvement of a Closed Chest Porcine Myocardial Infarction Model by Standardization of Tissue and Blood Sampling Procedures

Verbetering van een gesloten borst varkens myocardiaal infarct Model door standaardisatie van weefsel en bloed bemonsteringsprocedures

Myocardial ischemia reperfusion (I/R) injury contributes to almost half of the necrotic area after myocardial infarction. To date there is no approved ...

Isometric Contractility Measurement of the Mouse Mesenteric Artery Using Wire Myography

Isometrische Contractility meting van de muis mesenterische slagader met behulp van draad Myography

The wire myograph technique is used to assess the contractility of vascular smooth muscles in response to depolarization, GPCR agonists/inhibitors and ...