Name: mouse model of surgically-induced endometriosis by auto-transplantation of uterine tissue
Uploaded: 2012-01-06T12:00:00
Description: Watch this Scientific Journal Video about Mouse Model of Surgically-induced Endometriosis by Auto-transplantation of Uterine Tissue at JoVE.com

Un merci spécial à Chris et Audrey Kassotis Bailey pour un examen critique de ce manuscrit et au Dr Scott Korte, Joseph Beeman, Alison Curfman, Paul Kimball, Bridget Neibreggue, Jacob Redel, Amy Schroder, Maija Steinberg, et Stacey Winkeler pour leur aide dans l&#39;optimisation de ce modèle dans notre laboratoire. Le financement a été fourni par la clinique Grant Biodetectives Formation (NIH T90) (KEP), Université du Missouri Life Sciences Research Programme de premier cycle des chances, MU Conseil de recherches, MU subventions pour la recherche et de conseil NIH R21HD056441 (SCN).

1. La planification de la chirurgie d&#39;animaux vivants <ol><li> Assurez-vous que l&#39;approbation appropriée n&#39;a été reçue à travailler avec les animaux de laboratoire. </li><li> Ordre des souris et permettre au moins une semaine d&#39;acclimatation à leur nouvel environnement. </li><li> Des souris femelles logées en l&#39;absence d&#39;exposition à des phéromones mâles peut arrêter le vélo, un phénomène appelé l&#39;effet Whitten 15,16. Pour garder le vélo souris transfert trempé d...

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Steril. 93, 1615-1627 (2010).</li><li>Flores, I. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Molecular+profiling+of+experimental+endometriosis+identified+gene+expression+patterns+in+common+with+human+disease.">Molecular profiling of experimental endometriosis identified gene expression patterns in common with human disease.</a> Fertil. Steril. 87, 1180-1199 (2007).</li><li>Giudice, L. C., Kao, L. C. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Endometriosis.">Endometriosis.</a> Lancet. 364, 1789-1799 (2004).</li><li>Lin, Y. J., Lai, L. e. i., Y, H., Wing, L. Y. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Neutrophils+and+macrophages+promote+angiogenesis+in+the+early+stage+of+endometriosis+in+a+mouse+model.">Neutrophils and macrophages promote angiogenesis in the early stage of endometriosis in a mouse model.</a> Endocrinology. 147, 1278-1286 (2006).</li><li>Fang, Z. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Intact+progesterone+receptors+are+essential+to+counteract+the+proliferative+effect+of+estradiol+in+a+genetically+engineered+mouse+model+of+endometriosis.">Intact progesterone receptors are essential to counteract the proliferative effect of estradiol in a genetically engineered mouse model of endometriosis.</a> Fertil. Steril. 82, 673-678 (2004).</li><li>Fang, Z. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Genetic+or+enzymatic+disruption+of+aromatase+inhibits+the+growth+of+ectopic+uterine+tissue.">Genetic or enzymatic disruption of aromatase inhibits the growth of ectopic uterine tissue.</a> J. Clin. Endocrinol. Metab. 87, 3460-3466 (2002).</li><li>Cummings, A. M., Metcalf, J. L., Birnbaum, L. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Promotion+of+endometriosis+by+2,3,7,8-tetrachlorodibenzo-p-dioxin+in+rats+and+mice:+time-dose+dependence+and+species+comparison.">Promotion of endometriosis by 2,3,7,8-tetrachlorodibenzo-p-dioxin in rats and mice: time-dose dependence and species comparison.</a> Toxicol. Appl. Pharmacol. 138, 131-139 (1996).</li><li>Foster, W. 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E., Edwards, R. G. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Induction+of+superovulation+and+pregnancy+in+mature+mice+by+gonadotrophins.">Induction of superovulation and pregnancy in mature mice by gonadotrophins.</a> J. Endocrinol. 15, 374-384 (1957).</li><li>Wilson, E. D., Zarrow, M. X. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Comparison+of+superovulation+in+the+immature+mouse+and+rat.">Comparison of superovulation in the immature mouse and rat.</a> J. Reprod. Fertil. 3, 148-158 (1962).</li><li>Lee, B., Du, H., Taylor, H. S. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Experimental+murine+endometriosis+induces+DNA+methylation+and+altered+gene+expression+in+eutopic+endometrium.">Experimental murine endometriosis induces DNA methylation and altered gene expression in eutopic endometrium.</a> Biol. Reprod. 80, 79-85 (2009).</li><li>Somigliana, E. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Endometrial+ability+to+implant+in+ectopic+sites+can+be+prevented+by+interleukin-12+in+a+murine+model+of+endometriosis.">Endometrial ability to implant in ectopic sites can be prevented by interleukin-12 in a murine model of endometriosis.</a> Hum. Reprod. 14, 2944-2950 (1999).</li><li>Hirata, T. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Development+of+an+experimental+model+of+endometriosis+using+mice+that+ubiquitously+express+green+fluorescent+protein.">Development of an experimental model of endometriosis using mice that ubiquitously express green fluorescent protein.</a> Hum. Reprod. 20, 2092-2096 (2005).</li><li>Story, L., Kennedy, S. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Animal+studies+in+endometriosis:+a+review.">Animal studies in endometriosis: a review.</a> Ilar. J. 45, 132-138 (2004).</li><li>Cummings, A. M., Hedge, J. M., Birnbaum, L. S. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Effect+of+prenatal+exposure+to+TCDD+on+the+promotion+of+endometriotic+lesion+growth+by+TCDD+in+adult+female+rats+and+mice.">Effect of prenatal exposure to TCDD on the promotion of endometriotic lesion growth by TCDD in adult female rats and mice.</a> Toxicol. Sci. 52, 45-49 (1999).</li><li>Cummings, A. M., Metcalf, J. L. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Effects+of+estrogen,+progesterone,+and+methoxychlor+on+surgically+induced+endometriosis+in+rats.">Effects of estrogen, progesterone, and methoxychlor on surgically induced endometriosis in rats.</a> Fundam. Appl. Toxicol. 27, 287-290 (1995).</li><li>Sharpe-Timms, K. L. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Endometriotic+lesions+synthesize+and+secrete+a+haptoglobin-like+protein.">Endometriotic lesions synthesize and secrete a haptoglobin-like protein.</a> Biol. Reprod. 58, 988-994 (1998).</li><li>Yavuz, E., Oktem, M., Esinler, I., Toru, S. A., Zeyneloglu, H. B. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Genistein+causes+regression+of+endometriotic+implants+in+the+rat+model.">Genistein causes regression of endometriotic implants in the rat model.</a> Fertil. Steril. 88, 1129-1134 (2007).</li><li>Dmitrieva, N. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Endocannabinoid+involvement+in+endometriosis.">Endocannabinoid involvement in endometriosis.</a> Pain. 151, 703-710 (2010).</li><li>Efstathiou, J. A. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Nonsteroidal+antiinflammatory+drugs+differentially+suppress+endometriosis+in+a+murine+model.">Nonsteroidal antiinflammatory drugs differentially suppress endometriosis in a murine model.</a> Fertil. Steril. 83, 171-181 (2005).</li><li>Becker, C. M. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Endostatin+inhibits+the+growth+of+endometriotic+lesions+but+does+not+affect+fertility.">Endostatin inhibits the growth of endometriotic lesions but does not affect fertility.</a> Fertil. Steril. 84, 1144-1155 (2005).</li><li>Becker, C. M. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Short+synthetic+endostatin+peptides+inhibit+endothelial+migration+in+vitro+and+endometriosis+in+a+mouse+model.">Short synthetic endostatin peptides inhibit endothelial migration in vitro and endometriosis in a mouse model.</a> Fertil. Steril. 85, 71-77 (2006).</li><li>Sharpe-Timms, K. L. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Using+rats+as+a+research+model+for+the+study+of+endometriosis.">Using rats as a research model for the study of endometriosis.</a> Ann. N.Y. Acad. Sci. 955, 318-327 (2002).</li><li>Stilley, J. A., Woods-Marshall, R., Sutovsky, M., Sutovsky, P., Sharpe-Timms, K. L. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Reduced+Fecundity+in+Female+Rats+with+Surgically+Induced+Endometriosis+and+in+Their+Daughters:+A+Potential+Role+for+Tissue+Inhibitors+of+Metalloproteinase+1.">Reduced Fecundity in Female Rats with Surgically Induced Endometriosis and in Their Daughters: A Potential Role for Tissue Inhibitors of Metalloproteinase 1.</a> Biol. Reprod. 80, (2009).</li></ol>

Il ya plusieurs paramètres critiques qui doivent être notés dans l&#39;exercice de l&#39;induction chirurgicale de l&#39;endométriose chez la souris. Premièrement, l&#39;endométriose est une maladie oestrogènes dépendantes et en tant que telle cette chirurgie doit être réalisée chez les animaux intacts ou bien chez les animaux ayant subi une ovariectomie complétées avec des oestrogènes 20. Deuxièmement, la suture des biopsies endométriales à la cascade artérielle doit être effectuée avec u...

<table border="1"><tbody><tr><td> Nom du réactif / équipements </td><td> Société </td><td> Numéro de catalogue </td></tr><tr><td> Crayon de cire </td><td> Fisher </td><td> NC9954135 </td></tr><tr><td> Lame de verre </td><td> Fisher </td><td> 12-550-433 </td></tr><tr><td> Pipette </td><td> Fisher </td><td> S79383 </td></tr><tr><td> Microscope optique standard pour l&#39;évaluation des frottis vaginaux </td><td></td><td></td></tr><tr><td> La buprénorphine HCL C3 (CARJET) 10X1ml </td><td> Alimentation Butler Santé Animale </td><td> 022891 </td></tr><tr><td> Phosphate stérile saline tamponnée (PBS) </td><td> Gibco </td><td> 14040-117 </td></tr><tr><td> 10000 U / ml de pénicilline, 10.000 pg / ml de streptomycine dans le NaCl à 0,85% </td><td> Hyclone </td><td> SV30010 </td></tr><tr><td> L&#39;isoflurane </td><td> AbbottSanté Animale </td><td> 05260-05 </td></tr><tr><td> L&#39;isoflurane non réinhalation système d&#39;anesthésie </td><td></td><td></td></tr><tr><td> Recirculation pad chauffage à eau chaude </td><td></td><td></td></tr><tr><td> 30 ml gaine de seringues </td><td> Fisher </td><td> 14 à 823-16G </td></tr><tr><td> Poudre libre gants stériles </td><td> Fisherbrand </td><td> 19020558 </td></tr><tr><td> Pommade ophtalmique </td><td> Pharmaceuticals Major </td><td> 10033691 </td></tr><tr><td> Petite tondeuse électrique </td><td> Wahl </td><td> 9861-600 </td></tr><tr><td> Gommage chlorhexidine </td><td> Fisher </td><td> NC9863042 </td></tr><tr><td> Ethanol à 70% </td><td></td><td></td></tr><tr><td> Polylined champ stérile </td><td> Jetables Hôpital Busse </td><td> 696 </td></tr><tr><td> Taille 3 bistouri </td><td> Fisher </td><td> 22-079-657 </td></tr><tr><td> Numéro 10 lames de scalpel </td><td> Fisher </td><td> 22-079-681 </td></tr><tr><td> Petits ciseaux chirurgicaux </td><td> Roboz </td><td> RS-5850 </td></tr><tr><td> Petits dentelés semi-courbée pince </td><td> Roboz </td><td> RS-5135 </td></tr><tr><td> 5-0 soie noire tressée </td><td> Ethicon </td><td> K870H </td></tr><tr><td> Stérilisés en verre pyrex boîtes de Pétri </td><td> Corning </td><td> 70160-101 </td></tr><tr><td> 2 mm biopsie </td><td> Miltex </td><td> 33-31 </td></tr><tr><td> Gaze stérile </td><td> Kendall </td><td> 1806 </td></tr><tr><td> 6-0 suture noire en nylon monofilament Ethilon </td><td> Ethicon </td><td> 697G </td></tr><tr><td> Les conducteurs de seringues (en option) </td><td> Instruments de précision du monde </td><td> 500023 </td></tr><tr><td> 5-0 écru tresse couché vicryl de suture </td><td> Ethicon </td><td> J490G </td></tr><tr><td> 9mm Autoclip plaies clips </td><td> Becton Dickinson </td><td> 427631 </td></tr><tr><td> Autoclip applicateur &amp; Remover </td><td> Becton Dickinson </td><td> 427630 </td></tr><tr><td> Aiguille 23G </td><td> Becton Dickinson </td><td> 305193 </td></tr><tr><td> Seringue 1cc </td><td> Becton Dickson </td><td> 301025 </td></tr><tr><td> 5X se loupe (facultatif) </td><td> Fisher </td><td> 14-648-23 </td></tr><tr><td> 10% du formol tamponné </td><td> Fisher </td><td> SF100-4 </td></tr><tr><td> Étriers </td><td> Roboz </td><td> RS-6466 </td></tr><tr><td> Traitement / embedding cassettes </td><td> Fisher </td><td> 15 à 197-700A </td></tr><tr><td> Coussinets en mousse biopsie </td><td> Fisher </td><td> 22-038-222 </td></tr><tr><td> RNAqueous kit d&#39;isolement d&#39;ARN </td><td>Ambion </td><td> AM1912 </td></tr><tr><td> L&#39;azote liquide </td><td></td><td></td></tr><tr><td> Microcentrifugeuse bouchon rabattre à plat tube supérieur </td><td> Fisher </td><td> 02-681-240 </td></tr><tr><td> La kétamine (facultatif) </td><td> Simga </td><td> K4138 </td></tr><tr><td> Domitor (chlorhydrate de médétomidine) (facultatif) </td><td> Tocris </td><td> 2023 </td></tr><tr><td> Antisedan (atipamézole) (facultatif) </td><td> Sigma </td><td> A9611 </td></tr></tbody></table>

mouse model of surgically-induced endometriosis by auto-transplantation of uterine tissue

L&#39;endométriose est une maladie chronique, douloureuse dont l&#39;étiologie reste inconnue. Par ailleurs, le traitement de l&#39;endométriose peuvent exiger l&#39;enlèvement laparoscopique des lésions, et / ou chronique gestion pharmaceutique de la douleur et des symptômes d&#39;infertilité. Le coût associé à l&#39;endométriose a été estimé à 22 milliards de dollars par an dans le 1 États-Unis. Pour approfondir notre compréhension des mécanismes sous-jacents de cette maladie énigmatique, des modèles animaux ont été employées. Les primates se développent spontanément l&#39;endométriose et des modèles de primates conséquent ressemblent le plus à la maladie chez les femmes. Des modèles de rongeurs, cependant, sont plus rentables et facilement disponibles 2. Le modèle que nous décrivons ici implique un transfert autologue de tissu utérin au mésentère intestinal (Figure 1) et a été d&#39;abord développé chez le rat 3 et transféré plus tard à la souris 4. Le but du modèle de rongeur autologue de l&#39;endométriose chirurgicalement induite est d&#39;imiterla maladie chez les femmes. Nous et d&#39;autres ont déjà montré que le modèle gène modifié expression observée dans les lésions d&#39;endométriose chez les souris ou les rats qui miroirs observés chez les femmes avec la maladie 5,6. Un avantage de réaliser l&#39;intervention chez la souris est que l&#39;abondance des souches de souris transgéniques disponibles peuvent aider les chercheurs à déterminer le rôle des composants spécifiques important dans la création et la croissance de l&#39;endométriose. Un modèle alternatif dans lequel des fragments humains excisés endomètre sont introduits dans le péritoine de souris immunodéprimées est aussi largement utilisée, mais est limitée par l&#39;absence d&#39;un système immunitaire normal qui est pensé pour être important dans l&#39;endométriose 2,7. Surtout, le modèle de souris de l&#39;endométriose chirurgicalement induite est un modèle polyvalent qui a été utilisée pour étudier comment le système immunitaire 8, 9,10 hormones et des facteurs environnementaux affectent 11,12 endométriose ainsi que les effets de l&#39;endométriose sur Fertilité 13 et de la douleur 14.

Watch this Scientific Journal Video about Mouse Model of Surgically-induced Endometriosis by Auto-transplantation of Uterine Tissue at JoVE.com

Mouse Model of Surgically-induced Endometriosis by Auto-transplantation of Uterine Tissue

Une description de l&#39;induction chirurgicale de l&#39;endométriose chez les souris et les rats par auto-transplantation de tissu utérin à la cascade de l&#39;artère mésentère intestinal.

Modèle murin d&#39;endométriose chirurgicalement induite par Auto-transplantation de tissu utérin

Endometriosis is a chronic, painful disease whose etiology remains unknown. Furthermore, treatment of endometriosis can require laparoscopic removal of ...

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JoVE Journal

Medicine

Mouse Model of Intraluminal MCAO: Cerebral Infarct Evaluation by Cresyl Violet Staining

Modèle murin d&#39;MCAO intraluminale: Évaluation infarctus cérébral par coloration au violet de crésyl

Stroke is the third  cause of mortality and the leading cause of disability in the World. Ischemic  stroke accounts for approximately 80% of all strokes. ...

Recherche

Médecine

Implantation of Inferior Vena Cava Interposition Graft in Mouse Model

Implantation de veine cave inférieure interposition de greffe en modèle de souris

Biodegradable scaffolds seeded with bone marrow mononuclear cells (BMCs) are often used for reconstructive surgery to treat congenital cardiac anomalies. ...

Transplantation of Pulmonary Valve Using a Mouse Model of Heterotopic Heart Transplantation

Transplantation de valve pulmonaire en utilisant un modèle de souris de transplantation cardiaque hétérotopique

Tissue engineered heart valves, especially decellularized valves, are starting to gain momentum in clinical use of reconstructive surgery with mixed ...

Rose Bengal Photothrombosis by Confocal Optical Imaging In Vivo: A Model of Single Vessel Stroke

Rose Bengal Photothrombosis by Confocal Optical Imaging In Vivo:  A Model of Single Vessel Stroke

Rose Bengale Photothrombosis par imagerie optique confocal<em&gt; In Vivo</em&gt;: Un modèle de l&#39;unité des maladies du navire

In vivo imaging techniques have increased in utilization due to recent advances in imaging dyes and optical technologies, allowing for the ability to ...

Assessment of Cardiac Morphological and Functional Changes in Mouse Model of Transverse Aortic Constriction by Echocardiographic Imaging

Évaluation des cardiaques morphologiques et fonctionnelles changements dans le modèle de souris de Transverse Aortic Constriction par échocardiographie Imaging

Transverse aortic constriction (TAC) in mice has been used as a valuable model to study mechanisms of cardiac hypertrophy and heart failure1. A reliable ...

Creation of a Rodent Model of Abdominal Aortic Aneurysm by Blocking Adventitial Vasa Vasorum Perfusion

Création d’un modèle de rongeur d’anévrisme de l’aorte abdominale en bloquant adventitiels Vasa Vasorum Perfusion

The adventitial vasa vasorum (VV) provides oxygen and nourishment to the aortic wall. Hypoxia in the aortic wall can cause enlarged abdominal aortic ...

Contractility Measurements of Human Uterine Smooth Muscle to Aid Drug Development

Mesures de contractilité du Human Muscle lisse utérin à l’aide le développement de médicaments

Discovery and characterization of novel pharmaceutical compounds or biochemical probes rely on robust and physiologically relevant assay systems. We ...

A Mouse Model of Orthopedic Surgery to Study Postoperative Cognitive Dysfunction and Tissue Regeneration

Un modèle murin de la chirurgie orthopédique pour étudier la dysfonction Cognitive postopératoire et régénération des tissus

Surgery is commonly used to improve and maintain quality of life. Unfortunately, in vulnerable patients such as the elderly, complications may occur and ...

Improvement of a Closed Chest Porcine Myocardial Infarction Model by Standardization of Tissue and Blood Sampling Procedures

Amélioration d’un modèle de porcins infarctus du myocarde de thorax fermé par normalisation des tissus et des procédures d’échantillonnage de sang

Myocardial ischemia reperfusion (I/R) injury contributes to almost half of the necrotic area after myocardial infarction. To date there is no approved ...

Isometric Contractility Measurement of the Mouse Mesenteric Artery Using Wire Myography

Mesure de la contractilité isométrique de l’artère mésentérique de souris à l’aide de fil myographie

The wire myograph technique is used to assess the contractility of vascular smooth muscles in response to depolarization, GPCR agonists/inhibitors and ...