Name: mouse model of surgically-induced endometriosis by auto-transplantation of uterine tissue
Uploaded: 2012-01-06T12:00:00
Description: Watch this Scientific Journal Video about Mouse Model of Surgically-induced Endometriosis by Auto-transplantation of Uterine Tissue at JoVE.com

Besonderer Dank geht an Chris Kassotis und Audrey Bailey für die kritische Durchsicht des Manuskripts und Dr. Scott Korte, Joseph Beeman, Alison Curfman, Paul Kimball, Bridget Neibreggue, Jacob Redel, Amy Schröder, Maija Steinberg, und Stacey Winkeler für ihre Unterstützung bei der Optimierung dieses Modell in unserem Labor. Die Finanzierung wurde von der Clinical Biodetectives Training Grant (NIH T90) (KEP), University of Missouri Life Sciences Undergraduate Research Opportunities Program, MU Research Council, MU Research Board Zuschüsse und NIH R21HD056441 (SCN) zur Verfügung gestellt.

1. Die Planung für lebende Tiere Chirurgie <ol><li> Stellen Sie sicher, dass geeignete Genehmigung eingegangen ist, um mit Versuchstieren zu arbeiten. </li><li> Auftrag Mäusen und lassen Sie mindestens eine Woche Akklimatisierung an ihre neue Umgebung. </li><li> Weibliche Mäuse in der Abwesenheit von der Kontakt mit männlichen Pheromonen untergebracht ist Radfahren zu stoppen, bezeichnet ein Phänomen, das als Whitten-Effekt 15,16. Um Mäuse Rad übertragen Urin getränkten männlichen Betten zu den wei...

<ol>
	<li>Simoens, S., Hummelshoj, L., D'Hooghe, T. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Endometriosis:+cost+estimates+and+methodological+perspective.">Endometriosis: cost estimates and methodological perspective.</a> Hum. Reprod. Update. 13, 395-404 (2007).</li><li>Grummer, R. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Animal+models+in+endometriosis+research.">Animal models in endometriosis research.</a> Hum. Reprod. Update. 12, 641-649 (2006).</li><li>Vernon, M. W., Wilson, E. A. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Studies+on+the+surgical+induction+of+endometriosis+in+the+rat.">Studies on the surgical induction of endometriosis in the rat.</a> Fertil. Steril. 44, 684-694 (1985).</li><li>Cummings, A. M., Metcalf, J. L. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Induction+of+endometriosis+in+mice:+a+new+model+sensitive+to+estrogen.">Induction of endometriosis in mice: a new model sensitive to estrogen.</a> Reprod. Toxicol. 9, 233-238 (1995).</li><li>Pelch, K. E. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Aberrant+gene+expression+profile+in+a+mouse+model+of+endometriosis+mirrors+that+observed+in+women.">Aberrant gene expression profile in a mouse model of endometriosis mirrors that observed in women.</a> Fertil. Steril. 93, 1615-1627 (2010).</li><li>Flores, I. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Molecular+profiling+of+experimental+endometriosis+identified+gene+expression+patterns+in+common+with+human+disease.">Molecular profiling of experimental endometriosis identified gene expression patterns in common with human disease.</a> Fertil. Steril. 87, 1180-1199 (2007).</li><li>Giudice, L. C., Kao, L. C. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Endometriosis.">Endometriosis.</a> Lancet. 364, 1789-1799 (2004).</li><li>Lin, Y. J., Lai, L. e. i., Y, H., Wing, L. Y. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Neutrophils+and+macrophages+promote+angiogenesis+in+the+early+stage+of+endometriosis+in+a+mouse+model.">Neutrophils and macrophages promote angiogenesis in the early stage of endometriosis in a mouse model.</a> Endocrinology. 147, 1278-1286 (2006).</li><li>Fang, Z. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Intact+progesterone+receptors+are+essential+to+counteract+the+proliferative+effect+of+estradiol+in+a+genetically+engineered+mouse+model+of+endometriosis.">Intact progesterone receptors are essential to counteract the proliferative effect of estradiol in a genetically engineered mouse model of endometriosis.</a> Fertil. Steril. 82, 673-678 (2004).</li><li>Fang, Z. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Genetic+or+enzymatic+disruption+of+aromatase+inhibits+the+growth+of+ectopic+uterine+tissue.">Genetic or enzymatic disruption of aromatase inhibits the growth of ectopic uterine tissue.</a> J. Clin. Endocrinol. Metab. 87, 3460-3466 (2002).</li><li>Cummings, A. M., Metcalf, J. L., Birnbaum, L. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Promotion+of+endometriosis+by+2,3,7,8-tetrachlorodibenzo-p-dioxin+in+rats+and+mice:+time-dose+dependence+and+species+comparison.">Promotion of endometriosis by 2,3,7,8-tetrachlorodibenzo-p-dioxin in rats and mice: time-dose dependence and species comparison.</a> Toxicol. Appl. Pharmacol. 138, 131-139 (1996).</li><li>Foster, W. G. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Morphologic+characteristics+of+endometriosis+in+the+mouse+model:+application+to+toxicology.">Morphologic characteristics of endometriosis in the mouse model: application to toxicology.</a> Can. J. Physiol. Pharmacol. 75, 1188-1196 (1997).</li><li>Cummings, A. M., Metcalf, J. L. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Effect+of+surgically+induced+endometriosis+on+pregnancy+and+effect+of+pregnancy+and+lactation+on+endometriosis+in+mice.">Effect of surgically induced endometriosis on pregnancy and effect of pregnancy and lactation on endometriosis in mice.</a> Proc. Soc. Exp. Biol. Med. 212, 332-337 (1996).</li><li>Lu, Y., Nie, J., Liu, X., Zheng, Y., Guo, S. W. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Trichostatin+A,+a+histone+deacetylase+inhibitor,+reduces+lesion+growth+and+hyperalgesia+in+experimentally+induced+endometriosis+in+mice.">Trichostatin A, a histone deacetylase inhibitor, reduces lesion growth and hyperalgesia in experimentally induced endometriosis in mice.</a> Hum. Reprod. 25, 1014-1025 (2010).</li><li>Whitten, W. K. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Modification+of+the+oestrous+cycle+of+the+mouse+by+external+stimuli+associated+with+the+male.">Modification of the oestrous cycle of the mouse by external stimuli associated with the male.</a> J. Endocrinol. 13, 399-404 (1956).</li><li>Whitten, W. K., Bronson, F. H., Greenstein, J. A. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Estrus-inducing+pheromone+of+male+mice:+transport+by+movement+of+air.">Estrus-inducing pheromone of male mice: transport by movement of air.</a> Science. 161, 584-585 (1968).</li><li>Goldman, J. M., Murr, A. S., Cooper, R. L. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=The+rodent+estrous+cycle:+characterization+of+vaginal+cytology+and+its+utility+in+toxicological+studies.">The rodent estrous cycle: characterization of vaginal cytology and its utility in toxicological studies.</a> Birth. Defects. Res. B. Dev. Reprod. Toxicol. 80, 84-97 (2007).</li><li>Pritchett-Corning, K. R., Mulder, G. B., Luo, Y., White, W. J. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Principles+of+Rodent+Surgery+for+the+New+Surgeon.">Principles of Rodent Surgery for the New Surgeon.</a> J. Vis. Exp. (47), e2586-e2586 (2011).</li><li>Moen, M. H., Halvorsen, T. B. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Histologic+confirmation+of+endometriosis+in+different+peritoneal+lesions.">Histologic confirmation of endometriosis in different peritoneal lesions.</a> Acta. Obstet. Gynecol. Scand. 71, 337-342 (1992).</li><li>Cummings, A. M. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Methoxychlor+as+a+model+for+environmental+estrogens.">Methoxychlor as a model for environmental estrogens.</a> Crit. Rev. Toxicol. 27, 367-379 (1997).</li><li>Fowler, R. E., Edwards, R. G. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Induction+of+superovulation+and+pregnancy+in+mature+mice+by+gonadotrophins.">Induction of superovulation and pregnancy in mature mice by gonadotrophins.</a> J. Endocrinol. 15, 374-384 (1957).</li><li>Wilson, E. D., Zarrow, M. X. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Comparison+of+superovulation+in+the+immature+mouse+and+rat.">Comparison of superovulation in the immature mouse and rat.</a> J. Reprod. Fertil. 3, 148-158 (1962).</li><li>Lee, B., Du, H., Taylor, H. S. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Experimental+murine+endometriosis+induces+DNA+methylation+and+altered+gene+expression+in+eutopic+endometrium.">Experimental murine endometriosis induces DNA methylation and altered gene expression in eutopic endometrium.</a> Biol. Reprod. 80, 79-85 (2009).</li><li>Somigliana, E. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Endometrial+ability+to+implant+in+ectopic+sites+can+be+prevented+by+interleukin-12+in+a+murine+model+of+endometriosis.">Endometrial ability to implant in ectopic sites can be prevented by interleukin-12 in a murine model of endometriosis.</a> Hum. Reprod. 14, 2944-2950 (1999).</li><li>Hirata, T. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Development+of+an+experimental+model+of+endometriosis+using+mice+that+ubiquitously+express+green+fluorescent+protein.">Development of an experimental model of endometriosis using mice that ubiquitously express green fluorescent protein.</a> Hum. Reprod. 20, 2092-2096 (2005).</li><li>Story, L., Kennedy, S. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Animal+studies+in+endometriosis:+a+review.">Animal studies in endometriosis: a review.</a> Ilar. J. 45, 132-138 (2004).</li><li>Cummings, A. M., Hedge, J. M., Birnbaum, L. S. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Effect+of+prenatal+exposure+to+TCDD+on+the+promotion+of+endometriotic+lesion+growth+by+TCDD+in+adult+female+rats+and+mice.">Effect of prenatal exposure to TCDD on the promotion of endometriotic lesion growth by TCDD in adult female rats and mice.</a> Toxicol. Sci. 52, 45-49 (1999).</li><li>Cummings, A. M., Metcalf, J. L. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Effects+of+estrogen,+progesterone,+and+methoxychlor+on+surgically+induced+endometriosis+in+rats.">Effects of estrogen, progesterone, and methoxychlor on surgically induced endometriosis in rats.</a> Fundam. Appl. Toxicol. 27, 287-290 (1995).</li><li>Sharpe-Timms, K. L. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Endometriotic+lesions+synthesize+and+secrete+a+haptoglobin-like+protein.">Endometriotic lesions synthesize and secrete a haptoglobin-like protein.</a> Biol. Reprod. 58, 988-994 (1998).</li><li>Yavuz, E., Oktem, M., Esinler, I., Toru, S. A., Zeyneloglu, H. B. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Genistein+causes+regression+of+endometriotic+implants+in+the+rat+model.">Genistein causes regression of endometriotic implants in the rat model.</a> Fertil. Steril. 88, 1129-1134 (2007).</li><li>Dmitrieva, N. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Endocannabinoid+involvement+in+endometriosis.">Endocannabinoid involvement in endometriosis.</a> Pain. 151, 703-710 (2010).</li><li>Efstathiou, J. A. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Nonsteroidal+antiinflammatory+drugs+differentially+suppress+endometriosis+in+a+murine+model.">Nonsteroidal antiinflammatory drugs differentially suppress endometriosis in a murine model.</a> Fertil. Steril. 83, 171-181 (2005).</li><li>Becker, C. M. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Endostatin+inhibits+the+growth+of+endometriotic+lesions+but+does+not+affect+fertility.">Endostatin inhibits the growth of endometriotic lesions but does not affect fertility.</a> Fertil. Steril. 84, 1144-1155 (2005).</li><li>Becker, C. M. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Short+synthetic+endostatin+peptides+inhibit+endothelial+migration+in+vitro+and+endometriosis+in+a+mouse+model.">Short synthetic endostatin peptides inhibit endothelial migration in vitro and endometriosis in a mouse model.</a> Fertil. Steril. 85, 71-77 (2006).</li><li>Sharpe-Timms, K. L. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Using+rats+as+a+research+model+for+the+study+of+endometriosis.">Using rats as a research model for the study of endometriosis.</a> Ann. N.Y. Acad. Sci. 955, 318-327 (2002).</li><li>Stilley, J. A., Woods-Marshall, R., Sutovsky, M., Sutovsky, P., Sharpe-Timms, K. L. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Reduced+Fecundity+in+Female+Rats+with+Surgically+Induced+Endometriosis+and+in+Their+Daughters:+A+Potential+Role+for+Tissue+Inhibitors+of+Metalloproteinase+1.">Reduced Fecundity in Female Rats with Surgically Induced Endometriosis and in Their Daughters: A Potential Role for Tissue Inhibitors of Metalloproteinase 1.</a> Biol. Reprod. 80, (2009).</li></ol>

Es gibt mehrere kritische Parameter, bemerkt während der Durchführung der chirurgischen Induktion von Endometriose bei Mäusen sollte. Erstens ist die Endometriose ein Östrogen abhängig Krankheit und als solche diese Operation sollte in gesunden Tieren oder alternativ in ovarektomierten Tiere mit Östrogenen 20 ergänzt durchgeführt werden. Zweitens muss die Naht Endometriumbiopsien der arteriellen Kaskade mit äußerster Sorgfalt durchgeführt werden. Wir haben festgestellt, dass mit nur zwei relativ lo...

<table border="1"><tbody><tr><td> Name des Reagenzes / Ausrüstung </td><td> Firma </td><td> Katalog-Nummer </td></tr><tr><td> Wax Bleistift </td><td> Fischer </td><td> NC9954135 </td></tr><tr><td> Objektträger aus Glas </td><td> Fischer </td><td> 12-550-433 </td></tr><tr><td> Pipette </td><td> Fischer </td><td> S79383 </td></tr><tr><td> Standard-Lichtmikroskop für die Bewertung Vaginalzytologie Abstrichen </td><td></td><td></td></tr><tr><td> Buprenorphin HCL c3 (CARJET) 10X1ml </td><td> Butler Tiergesundheit Versorgung </td><td> 022891 </td></tr><tr><td> Sterile Phosphat-gepufferter Kochsalzlösung (PBS) </td><td> Gibco </td><td> 14040-117 </td></tr><tr><td> 10.000 U / ml Penicillin, 10.000 pg / ml Streptomycin in 0,85% NaCl </td><td> Hyclone </td><td> SV30010 </td></tr><tr><td> Isofluran </td><td> AbbottTiergesundheit </td><td> 05260-05 </td></tr><tr><td> Isofluran nicht Rückatmungssystemen System </td><td></td><td></td></tr><tr><td> Recirculating Warmwasser Heizkissen </td><td></td><td></td></tr><tr><td> 30 ml Spritze Mantel </td><td> Fischer </td><td> 14 bis 823-16G </td></tr><tr><td> Puderfrei sterile Handschuhe </td><td> Fisherbrand </td><td> 19020558 </td></tr><tr><td> Augensalbe </td><td> Große Pharmaceuticals </td><td> 10033691 </td></tr><tr><td> Kleine elektrische Haarschneidemaschine </td><td> Wahl </td><td> 9861-600 </td></tr><tr><td> Chlorhexidin scrub </td><td> Fischer </td><td> NC9863042 </td></tr><tr><td> 70% Ethanol </td><td></td><td></td></tr><tr><td> Polylined sterilen Bereich </td><td> Busse Krankenhaus Disposables </td><td> 696 </td></tr><tr><td> Größe 3 Skalpell </td><td> Fischer </td><td> 22-079-657 </td></tr><tr><td> Number 10 Skalpellklingen </td><td> Fischer </td><td> 22-079-681 </td></tr><tr><td> Kleine chirurgische Scheren </td><td> Roboz </td><td> RS-5850 </td></tr><tr><td> Kleine gezackte semi-gebogenen Pinzette </td><td> Roboz </td><td> RS-5135 </td></tr><tr><td> 5-0 schwarz geflochten Seidenfaden </td><td> Ethicon </td><td> K870H </td></tr><tr><td> Sterilisiert Pyrexglas Petrischalen </td><td> Corning </td><td> 70160-101 </td></tr><tr><td> 2 mm Biopsiestanze </td><td> Miltex </td><td> 33-31 </td></tr><tr><td> Sterile Gaze </td><td> Kendall </td><td> 1806 </td></tr><tr><td> 6-0 schwarz Monofilament Ethilon Nylonnaht </td><td> Ethicon </td><td> 697G </td></tr><tr><td> Needle-Treiber (optional) </td><td> World Precision Instruments </td><td> 500023 </td></tr><tr><td> 5-0 ungefärbt geflochten beschichtet vicryl Naht </td><td> Ethicon </td><td> J490G </td></tr><tr><td> 9mm Autoclip Wundklammern </td><td> Becton Dickinson </td><td> 427631 </td></tr><tr><td> Autoclip Applier &amp; Entferner </td><td> Becton Dickinson </td><td> 427630 </td></tr><tr><td> 23G Nadel </td><td> Becton Dickinson </td><td> 305193 </td></tr><tr><td> 1cc Spritze </td><td> Becton Dickson </td><td> 301025 </td></tr><tr><td> 5x Lupe Standfuß (optional) </td><td> Fischer </td><td> 14-648-23 </td></tr><tr><td> 10% gepuffertem Formalin </td><td> Fischer </td><td> SF100-4 </td></tr><tr><td> Taster </td><td> Roboz </td><td> RS-6466 </td></tr><tr><td> Verarbeitung / Einbettkassetten </td><td> Fischer </td><td> 15 bis 197-700A </td></tr><tr><td> Biopsie Schaumstoff-Pads </td><td> Fischer </td><td> 22-038-222 </td></tr><tr><td> RNAqueous RNA Isolation Kit </td><td>Ambion </td><td> AM1912 </td></tr><tr><td> Flüssiger Stickstoff </td><td></td><td></td></tr><tr><td> Schnappdeckel Mikrozentrifuge flachen Oberrohr </td><td> Fischer </td><td> 02-681-240 </td></tr><tr><td> Ketamin (optional) </td><td> Simga </td><td> K4138 </td></tr><tr><td> Domitor (Medetomidinhydrochlorid) (optional) </td><td> Tocris </td><td> 2023 </td></tr><tr><td> Antisedan (Atipamezol) (optional) </td><td> Sigma </td><td> A9611 </td></tr></tbody></table>

mouse model of surgically-induced endometriosis by auto-transplantation of uterine tissue

Die Endometriose ist eine chronische, schmerzhafte Erkrankung, deren Ätiologie ist unbekannt. Darüber hinaus kann die Behandlung der Endometriose erfordern laparoskopische Entfernung der Läsionen und / oder chronischen pharmazeutische Behandlung von Schmerzen und Unfruchtbarkeit Symptome. Die Kosten im Zusammenhang mit Endometriose hat bei 22 Milliarden Dollar pro Jahr in den Vereinigten Staaten von Amerika 1 geschätzt. Um unser Verständnis der zugrunde liegenden Mechanismen dieser rätselhaften Krankheit sind Tiermodelle eingesetzt. Primaten entwickeln spontan Endometriose und somit Primatenmodelle am ehesten die Krankheit bei Frauen ähneln. Nager-Modelle, sind aber kostengünstiger und leicht verfügbar 2. Das Modell, das wir hier beschreiben, beinhaltet eine autologe Transfer von Gebärmutter-Gewebe der Darmwand Mesenterium (Abbildung 1) und wurde zum ersten Mal in der Ratte 3 entwickelt und später an der Maus 4 übertragen. Das Ziel der autologen Nagetier Modell der chirurgisch-induzierten Endometriose ist zu imitierendie Krankheit bei Frauen. Wir und andere haben bereits gezeigt, dass die veränderte Genexpressionsmuster in Endometrioseherde von Mäusen oder Ratten Spiegel bei Frauen mit der Krankheit 5,6 beobachtet wurden. Ein Vorteil der Durchführung der Operation in der Maus ist, dass die Fülle von transgenen Mauslinien zur Verfügung können die Forscher bei der Bestimmung der Rolle von spezifischen Komponenten wichtig für die Gründung und das Wachstum der Endometriose helfen. Ein alternatives Modell, in dem ausgeschnittenen menschlichen Endometrium-Fragmente an das Bauchfell von immungeschwächten Mäusen eingebracht werden, ist ebenfalls weit verbreitet, sondern wird durch das Fehlen eines normalen Immunsystems, die Gedanken zu sein bei der Endometriose 2,7 wichtig ist begrenzt. Wichtig ist, dass die Maus-Modell der chirurgisch induzierten Endometriose ein vielseitiges Modell, das verwendet wurde, zu untersuchen, wie das Immunsystem 8, Hormone 9,10 und Umweltfaktoren 11,12 Endometriose sowie die Auswirkungen von Endometriose auf fert beeinflussenility 13 und Schmerzen 14.

Watch this Scientific Journal Video about Mouse Model of Surgically-induced Endometriosis by Auto-transplantation of Uterine Tissue at JoVE.com

Mouse Model of Surgically-induced Endometriosis by Auto-transplantation of Uterine Tissue

Eine Beschreibung der chirurgischen Induktion von Endometriose bei Mäusen und Ratten durch auto-Transplantation von Gebärmutter-Gewebe auf die arterielle Kaskade der Darm Mesenterium.

Maus-Modell der chirurgisch-induzierten Endometriose durch Auto-Transplantation von Gebärmutter-Gewebe

Endometriosis is a chronic, painful disease whose etiology remains unknown. Furthermore, treatment of endometriosis can require laparoscopic removal of ...

Research

JoVE Journal

Medicine

Mouse Model of Intraluminal MCAO: Cerebral Infarct Evaluation by Cresyl Violet Staining

Maus-Modell der Intraluminal MCAO: Hirninfarkt Evaluation durch Cresylviolett Staining

Stroke is the third  cause of mortality and the leading cause of disability in the World. Ischemic  stroke accounts for approximately 80% of all strokes. ...

Forschung

Medizin

Implantation of Inferior Vena Cava Interposition Graft in Mouse Model

Die Implantation von Vena Cava Interposition Graft in Maus-Modell

Biodegradable scaffolds seeded with bone marrow mononuclear cells (BMCs) are often used for reconstructive surgery to treat congenital cardiac anomalies. ...

Transplantation of Pulmonary Valve Using a Mouse Model of Heterotopic Heart Transplantation

Transplantation von Pulmonalklappe Mit einem Maus-Modell der heterotopen Herztransplantation

Tissue engineered heart valves, especially decellularized valves, are starting to gain momentum in clinical use of reconstructive surgery with mixed ...

Rose Bengal Photothrombosis by Confocal Optical Imaging In Vivo: A Model of Single Vessel Stroke

Rose Bengal Photothrombosis by Confocal Optical Imaging In Vivo:  A Model of Single Vessel Stroke

Rose Bengal Photothrombosis von konfokalen optischen Imaging<em&gt; In Vivo</em&gt;: Ein Modell des Einzelfahrzeug Stroke

In vivo imaging techniques have increased in utilization due to recent advances in imaging dyes and optical technologies, allowing for the ability to ...

Assessment of Cardiac Morphological and Functional Changes in Mouse Model of Transverse Aortic Constriction by Echocardiographic Imaging

Beurteilung der Herz Morphologische und funktionelle Veränderungen in Maus-Modell der Quer Aortakonstriktion durch Echokardiographie

Transverse aortic constriction (TAC) in mice has been used as a valuable model to study mechanisms of cardiac hypertrophy and heart failure1. A reliable ...

Creation of a Rodent Model of Abdominal Aortic Aneurysm by Blocking Adventitial Vasa Vasorum Perfusion

Erstellung eines Nagetier Modells der abdominalen Aortenaneurysma Vaskulogenese Adventitial Vasa Vasorum Perfusion

The adventitial vasa vasorum (VV) provides oxygen and nourishment to the aortic wall. Hypoxia in the aortic wall can cause enlarged abdominal aortic ...

Contractility Measurements of Human Uterine Smooth Muscle to Aid Drug Development

Kontraktilität Messungen der menschlichen Gebärmutter glatten Muskulatur um Hilfe Medikamentenentwicklung

Discovery and characterization of novel pharmaceutical compounds or biochemical probes rely on robust and physiologically relevant assay systems. We ...

A Mouse Model of Orthopedic Surgery to Study Postoperative Cognitive Dysfunction and Tissue Regeneration

Ein Maus-Modell der orthopädischen Chirurgie, Postoperative kognitive Dysfunktion und Geweberegeneration zu studieren

Surgery is commonly used to improve and maintain quality of life. Unfortunately, in vulnerable patients such as the elderly, complications may occur and ...

Improvement of a Closed Chest Porcine Myocardial Infarction Model by Standardization of Tissue and Blood Sampling Procedures

Verbesserung der einen geschlossenen Kasten porcinen Myokardinfarkt Modell durch Standardisierung der Gewebe und Blut Stichprobenverfahren

Myocardial ischemia reperfusion (I/R) injury contributes to almost half of the necrotic area after myocardial infarction. To date there is no approved ...

Isometric Contractility Measurement of the Mouse Mesenteric Artery Using Wire Myography

Messung der isometrischen Kontraktilität der Maus mesenterialen Arterie mit Draht Myographie

The wire myograph technique is used to assess the contractility of vascular smooth muscles in response to depolarization, GPCR agonists/inhibitors and ...